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排序方式: 共有292条查询结果,搜索用时 15 毫秒
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Islam MS Rahman MZ Khan SI Mahmud ZH Ramamurthy T Nair GB Sack RB Sack DA 《Microbiology and immunology》2005,49(8):779-784
The organization of the CTX prophage in environmental strains of Vibrio mimicus was investigated. Sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. Out of 1,600 isolates, 6 V. mimicus isolates contained ctxA gene. The organization of CTX prophage was determined by RFLP using ctxA probe. The CTX prophage integrated at a single site in V. mimicus genome which was present as a single copy flanked by at least a single RS element. Ribotype pattern revealed that a particular clone of V. mimicus acquired the CTXPhi in the aquatic environment. This study demonstrated that V. mimicus could act as a reservoir of CTXPhi in the aquatic environment. 相似文献
33.
Saha A Deb R Shah S Ramamurthy T Shinoda S Mukhophadyay AK Bhadra RK 《FEMS microbiology letters》2006,257(1):84-91
The bacterial chromosomal replication origin (ori) sequences are a highly conserved essential genetic element. In this study, the large chromosomal replication origin sequence of Vibrio cholerae (oriCIVC) has been targeted for identification of the organism, including the biotypes of serogroup O1. The oriCIVC sequence-based PCR assay specifically amplified an 890 bp fragment from all the V. cholerae strains examined. A point mutation in the oriCIVC sequence of the classical biotype of O1 serogroup led to the loss of a BglII site, which was utilized for differentiation from El Tor vibrios. Interestingly, the PCR assay amplified a similarly sized ori segment, designated as oriCIVM, from V. mimicus strains, but failed to produce any amplicon with other strains. Cloning and sequencing of the oriCIVM revealed high sequence similarity (96%) with oriCIVC. The results indicate that V. mimicus is indeed very closely related to V. cholerae. In addition, the BglII restriction fragment length polymorphism (RFLP) between oriCIVM and oriCIVC sequences allowed us to differentiate the two species. The ori sequence-based PCR-RFLP assay developed in this study appears to be a useful method for rapid identification and differentiation of V. cholerae and V. mimicus strains, as well as for the delineation of classical and El Tor biotypes of V. cholerae O1. 相似文献
34.
Analysis of Loss of heterozygosity and immunohistochemistry in BRCA1 gene in sporadic breast cancers
Dinesh KP Devaraj H Murugan V Rajaraman R Niranjali S 《Molecular and cellular biochemistry》2006,287(1-2):177-183
BRCA1 is a tumour suppressor gene (TSG), which predisposes cancer to both breast and ovary. The primary objective of the present study is to ascertain the involvement of BRCA1 gene in the pathogenesis of sporadic breast cancer women in Chennai (South India) by analysing its protein expression by immunohistochemistry (IHC) and loss of heterozygosity (LOH) for confirmation of the involvement of TSG in the study population. We found down regulation of BRCA1 protein (54%) in IHC and it was correlated with the clinicopathological parameters of the patients. We found near significant correlation (P < 0.063) between BRCA1 protein expression and clinicopathological parameters. We found 30% LOH in our study and it was also correlated with the clinicopathological parameters. No correlation was found between LOH and clinicopathological parameters. Though we found no correlation, the results revealed in this study support the involvement of BRCA1 TSG in the pathogenesis of sporadic breast cancer women in Chennai (South India). 相似文献
35.
Rengaswami Rajaraman Duane L Guernsey Murali M Rajaraman Selva R Rajaraman 《Cancer cell international》2006,6(1):25-26
We describe the basic tenets of the current concepts of cancer biology, and review the recent advances on the suppressor role
of senescence in tumor growth and the breakdown of this barrier during the origin of tumor growth. Senescence phenotype can
be induced by (1) telomere attrition-induced senescence at the end of the cellular mitotic life span (MLS*) and (2) also by
replication history-independent, accelerated senescence due to inadvertent activation of oncogenes or by exposure of cells
to genotoxins. Tumor suppressor genes p53/pRB/p16INK4A and related senescence checkpoints are involved in effecting the onset
of senescence. However, senescence as a tumor suppressor mechanism is a leaky process and senescent cells with mutations or
epimutations in these genes escape mitotic catastrophe-induced cell death by becoming polyploid cells. These polyploid giant
cells, before they die, give rise to several cells with viable genomes via nuclear budding and asymmetric cytokinesis. This
mode of cell division has been termed neosis and the immediate neotic offspring the Raju cells. The latter inherit genomic
instability and transiently display stem cell properties in that they differentiate into tumor cells and display extended,
but, limited MLS, at the end of which they enter senescent phase and can undergo secondary/tertiary neosis to produce the
next generation of Raju cells. Neosis is repeated several times during tumor growth in a non-synchronized fashion, is the
mode of origin of resistant tumor growth and contributes to tumor cell heterogeneity and continuity. The main event during
neosis appears to be the production of mitotically viable daughter genome after epigenetic modulation from the non-viable
polyploid genome of neosis mother cell (NMC). This leads to the growth of resistant tumor cells. Since during neosis, spindle
checkpoint is not activated, this may give rise to aneuploidy. Thus, tumor cells also are destined to die due to senescence,
but may escape senescence due to mutations or epimutations in the senescent checkpoint pathway. A historical review of neosis-like
events is presented and implications of neosis in relation to the current dogmas of cancer biology are discussed. Genesis
and repetitive re-genesis of Raju cells with transient "stemness" via neosis are of vital importance to the origin and continuous
growth of tumors, a process that appears to be common to all types of tumors. We suggest that unlike current anti-mitotic
therapy of cancers, anti-neotic therapy would not cause undesirable side effects. We propose a rational hypothesis for the
origin and progression of tumors in which neosis plays a major role in the multistep carcinogenesis in different types of
cancers. We define cancers as a single disease of uncontrolled neosis due to failure of senescent checkpoint controls. 相似文献
36.
Gopinath Balakrish Nair Thandavarayan Ramamurthy Dipika Sur Takashi Kurakawa Takuya Takahashi Koji Nomoto Yoshifumi Takeda 《Microbiology and immunology》2012,56(11):789-791
During a double‐blind, randomized, placebo‐controlled probiotic trial among 3758 children residing in an urban slum in Kolkata, India, Vibrio cholerae/mimicus was detected in fecal microbiota of healthy children. The importance of this finding in the local, regional and global transmission of cholera is discussed. 相似文献
37.
Senoh M Ghosh-Banerjee J Ramamurthy T Colwell RR Miyoshi S Nair GB Takeda Y 《Microbiology and immunology》2012,56(5):342-345
Viable but nonculturable (VBNC) Vibrio cholerae non-O1/non-O139, V. parahaemolyticus, enterohemorrhagic Escherichia coli, enterotoxigenic E. coli, enteropathogenic E. coli, Shigella flexneri, and Salmonella enterica were converted to the culturable state by co-culture with selected eukaryotic cells, e.g., HT-29, Caco-2, T84, HeLa, Intestine 407, and CHO cells. 相似文献
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