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221.
222.
Bakr Mayada E. Kashef Mona T. Hosny Alaa El-Dien M. S. Ramadan Mohammed A. 《International microbiology》2022,25(3):649-659
International Microbiology - Surface protein display C (SpdC) protein was described as a novel virulence factor of Staphylococcus aureus that affects biofilm formation and pathogenesis and favors... 相似文献
223.
Menha Swellam Amal Ramadan Enas A. El-Hussieny Noha M. Bakr Naglaa M. Hassan Mohamed Emam Sobeih Lobna R. EzzElArab 《Journal of cellular biochemistry》2019,120(8):12321-12330
microRNAs (miRNAs) are implicated in carcinogenesis and their expression in biological fluids offer great potential as nucleic acid markers for cancer detection and progression. Authors investigated the expression level of miRNAs (miRNA-21, miRNA-126, and miRNA-155) to evaluate their role as diagnostic and prognostic markers for breast cancer compared with other commonly used protein-based markers (CEA and CA15-3). Serum samples from patients with breast cancer (n = 96), patients with benign breast lesion (n = 47), and healthy individuals (n = 39) were enrolled for detection of miRNA expression levels and protein-based tumor markers using fluorescent real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Correlation among investigated markers with clinicopathological factors and clinical outcomes were determined. Expression of miRNA-21 and miRNA-155 revealed significant increases in patients with breast cancer compared with both benign and control groups, the same result was reported for tumor markers; on the other hand, miRNA-126 was significantly decreased in breast cancer group as compared with the other two groups. miRNA frequencies were significantly related to clinical staging and histological grading as compared with tumor markers. Patients with breast cancer with increased miRNA-21 and miRNA-155 and decreased miRNA-126 expressions had significantly worse disease-free survival, while only miRNA-21 and miRNA-126 showed poor OS (P< 0.005). In conclusion, investigated miRNAs were superior over tumor markers for the early stage of breast cancer especially those with high-risk factor and their assessment in blood facilitates their role as a potential prognostic molecular marker. 相似文献
224.
Cheng Cheng Lynsey MacIntyre Usama Ramadan Abdelmohsen Hannes Horn Paraskevi N. Polymenakou RuAngelie Edrada-Ebel Ute Hentschel 《PloS one》2015,10(9)
Marine sponge–associated actinomycetes are considered as promising sources for the discovery of novel biologically active compounds. In the present study, a total of 64 actinomycetes were isolated from 12 different marine sponge species that had been collected offshore the islands of Milos and Crete, Greece, eastern Mediterranean. The isolates were affiliated to 23 genera representing 8 different suborders based on nearly full length 16S rRNA gene sequencing. Four putatively novel species belonging to genera Geodermatophilus, Microlunatus, Rhodococcus and Actinomycetospora were identified based on a 16S rRNA gene sequence similarity of < 98.5% to currently described strains. Eight actinomycete isolates showed bioactivities against Trypanosma brucei brucei TC221 with half maximal inhibitory concentration (IC50) values <20 μg/mL. Thirty four isolates from the Milos collection and 12 isolates from the Crete collection were subjected to metabolomic analysis using high resolution LC-MS and NMR for dereplication purposes. Two isolates belonging to the genera Streptomyces (SBT348) and Micromonospora (SBT687) were prioritized based on their distinct chemistry profiles as well as their anti-trypanosomal activities. These findings demonstrated the feasibility and efficacy of utilizing metabolomics tools to prioritize chemically unique strains from microorganism collections and further highlight sponges as rich source for novel and bioactive actinomycetes. 相似文献
225.
Mohammed G. Ghonime Srabani Mitra Ramadan A. Eldomany Mark D. Wewers Mikhail A. Gavrilin 《PloS one》2015,10(5)
Inflammasome activation is a two-step process where step one, priming, prepares the inflammasome for its subsequent activation, by step two. Classically step one can be induced by LPS priming followed by step two, high dose ATP. Furthermore, when IL-18 processing is used as the inflammasome readout, priming occurs before new protein synthesis. In this context, how intracellular pathogens such as Francisella activate the inflammasome is incompletely understood, particularly regarding the relative importance of priming versus activation steps. To better understand these events we compared Francisella strains that differ in virulence and ability to induce inflammasome activation for their relative effects on step one vs. step two. When using the rapid priming model, i.e., 30 min priming by live or heat killed Francisella strains (step 1), followed by ATP (step 2), we found no difference in IL-18 release, p20 caspase-1 release and ASC oligomerization between Francisella strains (F. novicida, F. holarctica –LVS and F. tularensis Schu S4). This priming is fast, independent of bacteria viability, internalization and phagosome escape, but requires TLR2-mediated ERK phosphorylation. In contrast to their efficient priming capacity, Francisella strains LVS and Schu S4 were impaired in inflammasome triggering compared to F. novicida. Thus, observed differences in inflammasome activation by F. novicida, LVS and Schu S4 depend not on differences in priming but rather on their propensity to trigger the primed inflammasome. 相似文献
226.
Krishna K. Singh Fina Lovren Yi Pan Adrian Quan Azza Ramadan Pratiek N. Matkar Mehroz Ehsan Paul Sandhu Laura E. Mantella Nandini Gupta Hwee Teoh Matteo Parotto Arata Tabuchi Wolfgang M. Kuebler Mohammed Al-Omran Toren Finkel Subodh Verma 《The Journal of biological chemistry》2015,290(5):2547-2559
Pulmonary fibrosis is a progressive disease characterized by fibroblast proliferation and excess deposition of collagen and other extracellular matrix components. Although the origin of fibroblasts is multifactorial, recent data implicate endothelial-to-mesenchymal transition as an important source of fibroblasts. We report herein that loss of the essential autophagy gene ATG7 in endothelial cells (ECs) leads to impaired autophagic flux accompanied by marked changes in EC architecture, loss of endothelial, and gain of mesenchymal markers consistent with endothelial-to-mesenchymal transition. Loss of ATG7 also up-regulates TGFβ signaling and key pro-fibrotic genes in vitro. In vivo, EC-specific ATG7 knock-out mice exhibit a basal reduction in endothelial-specific markers and demonstrate an increased susceptibility to bleomycin-induced pulmonary fibrosis and collagen accumulation. Our findings help define the role of endothelial autophagy as a potential therapeutic target to limit organ fibrosis, a condition for which presently there are no effective available treatments. 相似文献
227.
Kolluru GR Green ZS Vredevoe LK Kuzma MR Ramadan SN Zosky MR 《Behavioural processes》2011,88(3):184-191
Parasites with indirect life cycles require trophic transmission from intermediate hosts to definitive (vertebrate) hosts. Transmission may be facilitated if parasite infection alters the behavior of intermediate hosts such that they are more vulnerable to predation. Vulnerability to predation may also be influenced by abiotic factors; however, rarely are the effects of parasites and abiotic factors examined simultaneously. The swash zone of sandy beaches is a particularly harsh environment. Sand crabs (Emerita analoga) burrow rapidly in the swash zone to avoid predators and dislodgment. We examined prevalence and abundance of the acanthocephalan parasite Profilicollis altmani in sand crabs, and investigated the synergistic effects of sand grain size (an important abiotic factor), parasite infection, body size and reproductive condition on burrowing speed in females, from three California sites. More heavily parasitized crabs burrowed more slowly, making them potentially more vulnerable to predation by marine bird definitive hosts. Ovigerous females harbored more parasites than non-ovigerous females, but burrowed more quickly. All crabs burrowed slowest in the coarsest sand, and burrowing times increased with repeated testing, suggesting that it is energetically costly. Abiotic and biotic factors influence burrowing, and behavioral variation across sites may reflect the response to natural variation in these factors. 相似文献
228.
Ramadan HA 《Molecular biology reports》2011,38(6):3929-3934
Characterization of molecular markers and the development of better assays for precise and rapid detection of domestic species
are always in demand. This is particularly due to recent food scares and the crisis of biodiversity resulting from the huge
ongoing illegal traffic of endangered species. The aim of this study was to develop a new and easy method for domestic species
identification (river buffalo, cattle, sheep and goat) based on the analysis of a specific mitochondrial nucleotide sequence.
For this reason, a specific fragment of Egyptian buffalo mitochondrial 16S rRNA gene (422 bp) was amplified by PCR using two
universal primers. The sequence of this specific fragment is completely conserved between all tested Egyptian buffaloes and
other river buffaloes in different places in the world. Also, the lengths of the homologous fragments were less by one nucleotide
(421 bp) in case of goats and two nucleotides (420 bp) in case of both cattle and sheep. The detection of specific variable
sites between investigated species within this fragment was sufficient to identify the biological origin of the samples. This
was achieved by alignment between the unknown homologous sequence and the reference sequences deposited in GenBank database
(accession numbers, FJ748599–FJ748607). Considering multiple alignment results between 16S rRNA homologous sequences obtained
from GenBank database with the reference sequence, it was shown that definite nucleotides are specific for each of the four
studied species of the family Bovidae. In addition, other nucleotides are detected which can allow discrimination between
two groups of animals belonging to two subfamilies of family Bovidae, Group one (closely related species like cattle and buffalo,
Subfamily Bovinae) and Group two (closely related species like sheep and goat, Subfamily Caprinae). This 16S DNA barcode character-based
approach could be used to complement cytochrome c oxidase I (COI) in DNA barcoding. Also, it is a good tool for identification
of unknown sample belonging to one of the four domestic animal species of family Bovidae quickly and easily. 相似文献
229.
Damaged DNA leads to genomic instability that causes many diseases such as cancer. Cells evolved the DNA damage response (DDR), which recognizes and efficiently repairs damaged DNA through the action of highly coordinated signalling mechanisms. Recently, a non-degradation-linked Lys(K)63-ubiquitin signal emerged as a signalling pathway essential for orchestration of the DDR after DNA double strand breaks (DSBs). How the ubiquitin-dependent proteasomal degradation system (UPS) coordinates DDR after DSBs is still poorly understood. Here, we review the evidence, suggesting the involvement of the degradation-linked K48-ubiquitin signal and the proteasome at the sites of DSBs. Based on this we propose the UPS as a central element in the orchestration of the DDR at the sites of DSBs. The suggested model is also discussed in the context of anti-cancer therapy. 相似文献
230.
Marsili A Aguayo-Mazzucato C Chen T Kumar A Chung M Lunsford EP Harney JW Van-Tran T Gianetti E Ramadan W Chou C Bonner-Weir S Larsen PR Silva JE Zavacki AM 《PloS one》2011,6(6):e20832