全文获取类型
收费全文 | 4126篇 |
免费 | 349篇 |
国内免费 | 2篇 |
出版年
2024年 | 2篇 |
2023年 | 14篇 |
2022年 | 38篇 |
2021年 | 58篇 |
2020年 | 37篇 |
2019年 | 46篇 |
2018年 | 63篇 |
2017年 | 54篇 |
2016年 | 103篇 |
2015年 | 194篇 |
2014年 | 211篇 |
2013年 | 250篇 |
2012年 | 342篇 |
2011年 | 329篇 |
2010年 | 201篇 |
2009年 | 187篇 |
2008年 | 236篇 |
2007年 | 287篇 |
2006年 | 300篇 |
2005年 | 279篇 |
2004年 | 237篇 |
2003年 | 235篇 |
2002年 | 214篇 |
2001年 | 40篇 |
2000年 | 40篇 |
1999年 | 49篇 |
1998年 | 77篇 |
1997年 | 43篇 |
1996年 | 33篇 |
1995年 | 37篇 |
1994年 | 27篇 |
1993年 | 28篇 |
1992年 | 27篇 |
1991年 | 21篇 |
1990年 | 20篇 |
1989年 | 11篇 |
1988年 | 10篇 |
1987年 | 14篇 |
1986年 | 11篇 |
1985年 | 10篇 |
1984年 | 11篇 |
1983年 | 10篇 |
1982年 | 12篇 |
1981年 | 8篇 |
1980年 | 2篇 |
1979年 | 8篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1967年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有4477条查询结果,搜索用时 62 毫秒
951.
952.
Inja Waldhauer Valeria Gonzalez-Nicolini Anne Freimoser-Grundschober Tapan K Nayak Linda Fahrni Ralf J. Hosse Danny Gerrits Edwin J. W. Geven Johannes Sam Sabine Lang Esther Bommer Virginie Steinhart Elisabeth Husar Sara Colombetti Erwin Van Puijenbroek Markus Neubauer J. Mark Cline Pradeep K. Garg Gregory Dugan Federica Cavallo Gonzalo Acuna Jehad Charo Volker Teichgrber Stefan Evers Otto C. Boerman Marina Bacac Ekkehard Moessner Pablo Umaa Christian Klein 《MABS-AUSTIN》2021,13(1)
953.
954.
A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria 总被引:1,自引:0,他引:1
Ralf Cord-Ruwisch 《Journal of microbiological methods》1985,4(1):33-36
Dissolved sulfide was determined spectrophotometrically as a colloidal solution of copper sulfide. Calibration curves were linear. Maximal deviation error was below 5%. Sulfide precipitated as FeS was determined after acidification of the medium. 相似文献
955.
956.
Meixin Tao Nitin K. Pandey Ryan Barnes Songi Han Ralf Langen 《Structure (London, England : 1993)》2019,27(10):1570-1580.e4
957.
Daniela Brünnert Marianne Kraus Thorsten Stühmer Stefanie Kirner Robin Heiden Pankaj Goyal Christoph Driessen Ralf C. Bargou Manik Chatterjee 《生物化学与生物物理学报:疾病的分子基础》2019,1865(6):1666-1676
Experimental data on resistance mechanisms of multiple myeloma (MM) to ixazomib (IXA), a second-generation proteasome inhibitor (PI), are currently lacking. We generated MM cell lines with a 10-fold higher resistance to IXA as their sensitive counterparts, and observed cross-resistance towards the PIs carfilzomib (CFZ) and bortezomib (BTZ). Analyses of the IXA-binding proteasome subunits PSMB5 and PSMB1 show increased PSMB5 expression and activity in all IXA-resistant MM cells, and upregulated PSMB1 expression in IXA-resistant AMO1 cells. In addition, sequence analysis of PSMB5 revealed a p.Thr21Ala mutation in IXA-resistant MM1.S cells, and a p.Ala50Val mutation in IXA-resistant L363 cells, whereas IXA-resistant AMO1 cells lack PSMB5 mutations. IXA-resistant cells retain their sensitivity to therapeutic agents that mediate cytotoxic effects via induction of proteotoxic stress. Induction of ER stress and apoptosis by the p97 inhibitor CB-5083 was strongly enhanced in combination with the PI3Kα inhibitor BYL-719 or the HDAC inhibitor panobinostat suggesting potential therapeutic strategies to circumvent IXA resistance in MM. Taken together, our newly established IXA-resistant cell lines provide first insights into resistance mechanisms and overcoming treatment strategies, and represent suitable models to further study IXA resistance in MM. 相似文献
958.
Stadler V Beyer M König K Nesterov A Torralba G Lindenstruth V Hausmann M Bischoff FR Breitling F 《Journal of proteome research》2007,6(8):3197-3202
Complementary metal oxide semiconductor (CMOS) microelectronic chips fulfill important functions in the field of biomedical research, ranging from the generation of high complexity DNA and protein arrays to the detection of specific interactions thereupon. Nevertheless, the issue of merging pure CMOS technology with a chemically stable surface modification which further resists interfering nonspecific protein adsorption has not been addressed yet. We present a novel surface coating for CMOS microchips based on poly(ethylene glycol)methacrylate graft polymer films, which in addition provides high loadings of functional groups for the linkage of probe molecules. The coated microchips were compatible with the harshest conditions emerging in microarray generating methods, thoroughly retaining structural integrity and microelectronic functionality. Nonspecific adsorption of proteins on the chip's surface was completely obviated even with complex serum protein mixtures. We could demonstrate the background-free antibody staining of immobilized probe molecules without using any blocking agents, encouraging further integration of CMOS technology in proteome research. 相似文献
959.
Proteomics characterization of mouse kidney peroxisomes by tandem mass spectrometry and protein correlation profiling 总被引:2,自引:0,他引:2
Wiese S Gronemeyer T Ofman R Kunze M Grou CP Almeida JA Eisenacher M Stephan C Hayen H Schollenberger L Korosec T Waterham HR Schliebs W Erdmann R Berger J Meyer HE Just W Azevedo JE Wanders RJ Warscheid B 《Molecular & cellular proteomics : MCP》2007,6(12):2045-2057
The peroxisome represents a ubiquitous single membrane-bound key organelle that executes various metabolic pathways such as fatty acid degradation by alpha- and beta-oxidation, ether-phospholipid biosynthesis, metabolism of reactive oxygen species, and detoxification of glyoxylate in mammals. To fulfil this vast array of metabolic functions, peroxisomes accommodate approximately 50 different enzymes at least as identified until now. Interest in peroxisomes has been fueled by the discovery of a group of genetic diseases in humans, which are caused by either a defect in peroxisome biogenesis or the deficient activity of a distinct peroxisomal enzyme or transporter. Although this research has greatly improved our understanding of peroxisomes and their role in mammalian metabolism, deeper insight into biochemistry and functions of peroxisomes is required to expand our knowledge of this low abundance but vital organelle. In this work, we used classical subcellular fractionation in combination with MS-based proteomics methodologies to characterize the proteome of mouse kidney peroxisomes. We could identify virtually all known components involved in peroxisomal metabolism and biogenesis. Moreover through protein localization studies by using a quantitative MS screen combined with statistical analyses, we identified 15 new peroxisomal candidates. Of these, we further investigated five candidates by immunocytochemistry, which confirmed their localization in peroxisomes. As a result of this joint effort, we believe to have compiled the so far most comprehensive protein catalogue of mammalian peroxisomes. 相似文献
960.
Conservation of PEX19-binding motifs required for protein targeting to mammalian peroxisomal and trypanosome glycosomal membranes
下载免费PDF全文
![点击此处可从《Eukaryotic cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Saveria T Halbach A Erdmann R Volkmer-Engert R Landgraf C Rottensteiner H Parsons M 《Eukaryotic cell》2007,6(8):1439-1449
Glycosomes are divergent peroxisomes found in trypanosomatid protozoa, including those that cause severe human diseases throughout much of the world. While peroxisomes are dispensable for both yeast (Saccharomyces cerevisiae and others) and mammalian cells in vitro, glycosomes are essential for trypanosomes and hence are viewed as a potential drug target. The import of proteins into the matrix of peroxisomes utilizes multiple peroxisomal membrane proteins which require the peroxin PEX19 for insertion into the peroxisomal membrane. In this report, we show that the specificity of peroxisomal membrane protein binding for Trypanosoma brucei PEX19 is very similar to those previously identified for human and yeast PEX19. Our studies show that trafficking is conserved across these distant phyla and that both a PEX19 binding site and a transmembrane domain are required for the insertion of two test proteins into the glycosomal membrane. However, in contrast to T. brucei PEX10 and PEX12, T. brucei PEX14 does not traffic to human peroxisomes, indicating that it is not recognized by the human PEX14 import mechanism. 相似文献