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681.
682.
Seasonal culture of dormant reproductive buds ofSalix tetrasperma: Analysis of the flowering process
The flowering process in a female tree ofSalix tetrasperma was analysed by culturing its reproductive buds at different developmental stages during the dormant period on a chemically
defined medium and examining the nature of sprouts produced by them. Buds at the upper eight nodes of the actively growing
shoots developing in an acropetal sequence were cultured in separate lots. While all the buds collected from the 1st and 2nd
nodes of the branches from the top downwards were vegetative and produced shoots, a considerable number of those collected
from the 3rd and 4th nodes were reproductively determined and produced catkins. All the buds obtained from the 5th node and
below were reproductive.
Reproductive buds were cultured at regular time intervals during the dormant period. Freshly formed buds cultured in March
during the spring growth flush produced catkins and were therefore reproductively determined. However, such a determination
was not tantamount to flowering, as the floral meristems present in the axils of catkin bracts remained quiescent. Floral
meristems of the buds cultured during April to August developed into small vegetative shoots. This was followed by the crucial
period during September to December when the hitherto vegetative sprouts of the floral meristems showed a gradual transition
into ovaries (female flowers) resulting in fertile catkins. Catkins produced from buds cultured in January and February produced
well-developed ovaries. 相似文献
683.
Aaron James Camp Rajiv Wijesinghe 《The international journal of biochemistry & cell biology》2009,41(11):2118-2121
Calretinin is a member of the calcium-binding protein EF-hand family first identified in the retina. As with the other 200-plus calcium-binding proteins, calretinin serves a range of cellular functions including intracellular calcium buffering, messenger targeting, and is involved in processes such as cell cycle arrest, and apoptosis. Calcium-binding proteins including calretinin are expressed differentially in neuronal subpopulations throughout the vertebrate and invertebrate nervous system and their expression has been used to selectively target specific cell types and isolate neuronal networks. More recent experiments have revealed that calretinin plays a crucial role in the modulation of intrinsic neuronal excitability and the induction of long-term potentiation (LTP). Furthermore, selective knockout of calretinin in mice produces disturbances of motor coordination and suggests a putative role for calretinin in the maintenance of calcium dynamics underlying motor adaptation. 相似文献
684.
Essential trace elements such as iron (Fe) are known to interact with nonessential metals like lead (Pb), influencing its
metabolism. Ferric chloride and lead nitrate were administered intraperitoneally to Swiss albino miceMus musculus singly and successively, with or without a time gap of 1 h, to study the degree of protection, if any, afforded by iron against
the clastogenic effects induced by Pb in bone marrow cells. A decrease in the frequency of lead-induced chromosomal aberrations
was observed when Fe was given together with or prior to Pb administration. 相似文献
685.
The entrapment of residual hydrocarbon ganglia during water table fluctuations can produce a long‐term contamination threat to groundwater supplies that is difficult to remove. The mobilization of entrapped hydrocarbon ganglia depends on the balance between capillary and gravitational forces represented by the Bond number. The present work focuses on the influence of the interfacial tension between the hydrocarbon and the surrounding water on the entrapment and mobilization of the residual ganglia. Laboratory column tests using glass beads as the porous medium have been conducted to determine the residual saturation of a hydrocarbon (Soltrol 170) trapped during vertical displacements due to a rising water table and the necessary decrease in interfacial tension to mobilize these trapped ganglia. The interfacial tension was decreased by the addition of isopropyl alcohol to the water phase. Saturations of the three phases (water, hydrocarbon, and air) were measured with a dual‐beam y‐densitometer. The results for residual hydrocarbon saturation at various interfacial tensions were combined with previous results for different particle diameters to provide a general relationship between residual saturation and Bond number. The relationship is expressed in an empirical correlation valid for Bond numbers between 0.001 and 1.2. 相似文献
686.
Hawley E. Kunz Jessica M. Dorschner Taylor E. Berent Thomas Meyer Xuewei Wang Aminah Jatoi Rajiv Kumar Ian R. Lanza 《The Journal of biological chemistry》2020,295(51):17441
Cancer cachexia is characterized by reductions in peripheral lean muscle mass. Prior studies have primarily focused on increased protein breakdown as the driver of cancer-associated muscle wasting. Therapeutic interventions targeting catabolic pathways have, however, largely failed to preserve muscle mass in cachexia, suggesting that other mechanisms might be involved. In pursuit of novel pathways, we used untargeted metabolomics to search for metabolite signatures that may be linked with muscle atrophy. We injected 7-week–old C57/BL6 mice with LLC1 tumor cells or vehicle. After 21 days, tumor-bearing mice exhibited reduced body and muscle mass and impaired grip strength compared with controls, which was accompanied by lower synthesis rates of mixed muscle protein and the myofibrillar and sarcoplasmic muscle fractions. Reductions in protein synthesis were accompanied by mitochondrial enlargement and reduced coupling efficiency in tumor-bearing mice. To generate mechanistic insights into impaired protein synthesis, we performed untargeted metabolomic analyses of plasma and muscle and found increased concentrations of two methylarginines, asymmetric dimethylarginine (ADMA) and NG-monomethyl-l-arginine, in tumor-bearing mice compared with control mice. Compared with healthy controls, human cancer patients were also found to have higher levels of ADMA in the skeletal muscle. Treatment of C2C12 myotubes with ADMA impaired protein synthesis and reduced mitochondrial protein quality. These results suggest that increased levels of ADMA and mitochondrial changes may contribute to impaired muscle protein synthesis in cancer cachexia and could point to novel therapeutic targets by which to mitigate cancer cachexia. 相似文献