全文获取类型
收费全文 | 990篇 |
免费 | 41篇 |
国内免费 | 1篇 |
专业分类
1032篇 |
出版年
2023年 | 6篇 |
2022年 | 9篇 |
2021年 | 20篇 |
2020年 | 13篇 |
2019年 | 14篇 |
2018年 | 21篇 |
2017年 | 19篇 |
2016年 | 13篇 |
2015年 | 37篇 |
2014年 | 42篇 |
2013年 | 67篇 |
2012年 | 82篇 |
2011年 | 80篇 |
2010年 | 39篇 |
2009年 | 37篇 |
2008年 | 46篇 |
2007年 | 62篇 |
2006年 | 59篇 |
2005年 | 47篇 |
2004年 | 52篇 |
2003年 | 39篇 |
2002年 | 32篇 |
2001年 | 10篇 |
2000年 | 8篇 |
1999年 | 7篇 |
1998年 | 14篇 |
1997年 | 8篇 |
1996年 | 6篇 |
1995年 | 10篇 |
1994年 | 8篇 |
1993年 | 6篇 |
1991年 | 9篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 5篇 |
1987年 | 8篇 |
1985年 | 4篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1981年 | 11篇 |
1980年 | 8篇 |
1979年 | 9篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 5篇 |
1974年 | 3篇 |
1967年 | 2篇 |
1966年 | 2篇 |
1965年 | 2篇 |
排序方式: 共有1032条查询结果,搜索用时 15 毫秒
991.
992.
993.
Srishylam Penjarla S. Rajendra Prasad Dhande Sudhakar Reddy Santhosh Penta Yogesh S. Sanghvi 《Nucleosides, nucleotides & nucleic acids》2018,37(4):232-247
Regioselective protection of primary hydroxyl group in nucleoside and carbohydrate analogs was accomplished using dimethoxytrityl alcohol (DMTr-OH) or dimethylpixyl alcohol (DMPx-OH) in presence of copper(II)nitrate as a Lewis acid catalyst. Excellent selectivity was observed for the protection of primary hydroxyl group over secondary while glycosidic bond remain unaffected. Utility of this methodology was further exemplified via DMTr- and DMPx-protection of alipahtic acyclic and cyclic diols. 相似文献
994.
Calcineurin (CaN), also known as calmodulin-dependent phosphatase, was cloned from bovine cardiac muscle and the deduced amino acid sequences of CaN A revealed that it had an open reading frame of 511 amino acid residues. As compared to bovine brain CaN A, the cardiac enzyme contains a 10 amino acid (ATVEAIEADE) deletion before the autoinhibitory region. A deletion analysis of the catalytic domain revealed a 20% decrease in phosphatase activity when the N-terminal 200 amino acids were removed from CaN A as compared to the wild type enzyme. The C-terminal deletions of CaN A revealed that in addition to the autoinhibitory domain (residues 457-480), additional adjacent residues (407-456) also inhibited CaN activity. These results point to either a second autoinhibitory region within CaN A or an extension of the previously noted autoinhibitory region within the cardiac CaN A enzyme. 相似文献
995.
A strain of Bacillus subtilis was able to grow and produce a biosurfactant on 2% sucrose at 45°C. As a result of biosurfactant synthesis the surface tension
of the medium was reduced from 68 dynes cm−1 to 28 dynes cm−1. The strain had the capacity to produce the biosurfactant at high NaCl concentrations (4%) and a wide range of pH (4.5–10.5).
The biosurfactant retained its surface-active properties after heating at 100°C for 2 h and at different pH values (4.5–10.5).
A maximum amount of biosurfactant was produced when urea or nitrate ions were supplied as nitrogen source. The use of the
biosurfactant at high temperatures, acidic, alkaline and saline environments is discussed. As a result of its action, 62%
of oil in a sand pack column could be recovered, indicating its potential application in microbiologically enhanced oil
recovery.
Received 28 March 1996/ Accepted in revised form 16 September 1996 相似文献
996.
The interest in microbial biosurfactants has steadily increased during the past decade. In addition to the classical application as emulsifiers of hydrocarbons, they can be used in environmental protection, crude-oil recovery, food-processing industries and in various fields of biomedicine. Biosurfactants have several advantages over chemical surfactants including lower toxicity and higher biodegradability, and are likely to become molecules of the future in areas such as biomedicine and therapeutics. Here, we discuss the role and applications of biosurfactants (mainly glycolipids and lipopeptides) focusing on medicinal and therapeutic perspectives. 相似文献
997.
Holsworth DD Cai C Cheng XM Cody WL Downing DM Erasga N Lee C Powell NA Edmunds JJ Stier M Jalaie M Zhang E McConnell P Ryan MJ Bryant J Li T Kasani A Hall E Subedi R Rahim M Maiti S 《Bioorganic & medicinal chemistry letters》2006,16(9):2500-2504
A systematic investigation of the S3 sub-pocket activity requirements was conducted. It was observed that linear and sterically small side chain substituents are preferred in the S3 sub-pocket for optimal renin inhibition. Polar groups in the S3-sub-pocket were not well tolerated and caused a reduction in renin inhibitory activity. Further, compounds with clog P's < or = 3 demonstrated a dramatic reduction in CYP3A4 inhibitory activity. 相似文献
998.
Morabito F Damle RN Deaglio S Keating M Ferrarini M Chiorazzi N 《Molecular medicine (Cambridge, Mass.)》2006,12(11-12):342-344
One aim of this session given at the Torino CD38 Meeting in June, 2006 was to review the role of CD38 in B-cell Chronic Lymphocytic Leukemia (B-CLL), and its potential as a therapeutic target. CD38(high) B-CLL cases show activated phenotypic features as compared with CD38(low) cases. Moreover, a greater percentage of Ki-67 and telomerase activity is documented among CD38(high) cases. Also, CD38 is not merely a negative prognostic marker in B-CLL, but also a key element in the pathogenetic network underlying the disease. A large series of B-CLL cases investigating the CD38 expression on bone marrow B-cells identified CD38 value <10% as the cut-off predicting a longer time to treatment. However, neither CD38 nor ZAP-70 by themselves or in combination were able to anticipate IgVH mutational status. Transferring these findings into clinical ground, 3 groups of B-CLL cases were identified with significantly different clinical courses: i.e., low-risk (no negative prognostic factor), intermediate-risk (1 negative prognostic factor) and high-risk (2-3 negative prognostic factors) patients. Altogether these results suggest that: i) CD38-expressing cells present not only an activation status, but also a different stage differentiation with a more repeated turnover; ii) CD38 contributes to controlling a signaling pathway that confers to B-CLL cells an increased proliferative potential, enhancing aggressiveness of this variant; iii) different CD38 cut off values should be considered for peripheral blood and bone marrow; iv) CD38 seems to independently contribute to prognostic stratification of B-CLL. 相似文献
999.
Arpana Kumari Balwinder Kaur Rajendra Srivastava Rajender S. Sangwan 《Biochemistry and Biophysics Reports》2015
Alkaline protease from brinjal leaf (Solanum melongena) having milk clotting activity has been purified to 9.44 fold to a final specific activity of 45.71 U/mg. SDS-PAGE of the final preparation revealed a single protein band of approx 14 kDa. Purified enzyme was characterized and was successfully immobilized into the amorphous mesoporous silica (SBA-15) and crystalline mesoporous zeolite (Nano-ZSM-5) using entrapment method. Maximum immobilization of 63.5% and 79.77% was obtained with SBA-15 and Nano-ZSM-5, respectively. This protocol serves as a novel approach for bioprocesses, mainly as milk coagulant for local dairy products and particularly, cheese making, and opens the new dimension of further research and other innovation. 相似文献
1000.
Soopaya R Stringer LD Woods B Stephens AE Butler RC Lacey I Kaur A Suckling DM 《Journal of economic entomology》2011,104(6):1999-2008
The radiation biology of two geographically isolated populations of the light brown apple moth [Epiphyas postvittana (Walker)] was studied in Australia and New Zealand as an initiation of a SIT/F1 sterility program. Pharate and < or = 2 d pre-emergence pupae were exposed to increasing radiation doses up to a maximum dose of 300 Gy. Fertility and other life history parameters were measured in emerging adults (parental) and their progeny (F1-F3 adults). Parental fecundity was significantly affected by increasing irradiation dose in pharate pupae only. For both populations, parental egg fertility declined with increasing radiation. This was most pronounced for the irradiated parental females whose fertility declined at a higher rate than of irradiated males. At 250 Gy, females < or = 2 d preemergence pupae produced few larvae and no adults at F1. No larvae hatched from 250 Gy-irradiated female pharate pupae. At 300 Gy, males still had residual fertility of 2-5.5%, with pharate pupae being the more radio-sensitive. Radiation-induced deleterious inherited effects in offspring from irradiated males were expressed as increased developmental time in F1 larvae, a reduction in percent F1 female survival, decreased adult emergence and increased cumulative mortality over subsequent generations. Males irradiated at > or = 150 Gy produced few but highly sterile offspring at F1 and mortality was > 99% by F2 egg. 相似文献