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361.
In India, Mass Drug Administration is on going towards elimination of lymphatic filariasis in many areas, which might lead to intense selection pressure on the parasite populations and their genetic restructuring. This calls for molecular finger printing of Wuchereria bancrofti parasite populations at national level and monitoring genetic changes in the future. For this purpose a reliable, less expensive, rapid, and reproducible molecular tool is necessary, which is not available for W. bancrofti at this time. We identified robust molecular markers based on the comparison of random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) profiles and the genetic data generated from parasite populations collected from areas in Northern (Varanasi, Uttar Pradesh state), Southern (Kozhikode, Kerala State) and Central regions (Jagdalpur, Chattisgarh state) of India, where lymphatic filariasis is endemic for many decades. RAPD profiles for these parasite populations were generated using three different primers and the dendrograms constructed using the profiles were all different. In order to identify appropriate RAPD primer(s), we compared the results of RAPD with the fingerprint profile and genetic data obtained by the more reliable AFLP technique, using the parasite populations from the same areas. RAPD marker (OP8) primer produced phylogenetic data almost similar to that of AFLP analysis. The marker was able to reveal variations between the parasite populations collected from Varanasi, Kozhikode, and Jagdalpur. Most importantly, RAPD primer OP8 produced reproducible results, when tested in three different trials. In view of the limited availability of W. bancrofti parasite DNA, along with a lower cost and ease of performance, RAPD appears to be more suitable compared to AFLP at the present juncture, since complete genome information of this parasite is still not available. Thus, RAPD primer OP8 can be a very useful molecular maker for DNA finger printing of W. bancrofti populations at present. 相似文献
362.
Patra Sinjini Saxena Shivam Sahu Nilanjan Pradhan Biswaranjan Roychowdhury Anasuya 《Probiotics and antimicrobial proteins》2021,13(4):1138-1156
Probiotics and Antimicrobial Proteins - With the alarming rise of infected cases and deaths, COVID-19 is a pandemic, affecting 220 countries worldwide. Until now, no specific treatment is available... 相似文献
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Liu Yongliang Patra Barunava Singh Sanjay Kumar Paul Priyanka Zhou Yan Li Yongqing Wang Ying Pattanaik Sitakanta Yuan Ling 《Biotechnology letters》2021,43(11):2085-2103
Biotechnology Letters - Plants synthesize a vast array of specialized metabolites that primarily contribute to their defense and survival under adverse conditions. Many of the specialized... 相似文献
365.
Patagar Dayanand Uttarkar Akshay Patra Swarna M. Patil Jagadish H. Kusanur Raviraj Niranjan Vidya Kumar H. G. Ashok 《Russian Journal of Bioorganic Chemistry》2021,47(2):390-398
Russian Journal of Bioorganic Chemistry - 1,5-Benzodiazepines are one of the important class of tranquilizers. The fusion of heterocyclic systems like coumarins and indolines with the... 相似文献
366.
Gitishree Das Han-Seung Shin Anuj Kumar Chethala N. Vishnuprasad Jayanta Kumar Patra 《Saudi Journal of Biological Sciences》2021,28(1):980
Presently, photo-mediated optimized synthesis of SNPs (CS-AgNPs) was carried out with the help of aqueous extracts of coconut (Cocos nucifera) outer shell fibre. Green synthesis of CS-AgNPs was undertaken under laboratory light conditions and characterized by several standard techniques such as UV–visible spectrophotometer (UV–Vis), X-ray diffraction pattern (XRD), Fourier transform infrared (FT-IR), and scanning electron microscopy (SEM) images and energy dispersive spectroscopy (EDX). UV–Vis spectra displayed a surface plasmon resonance peak at 468 nm equivalent to CS-AgNPs, and the FT-IR spectra confirmed the association of biological molecules from the extract in the synthesis process. The SEM image data confirmed the round and circular nature of CS-AgNPs. The EDX data presented the elemental configuration with a solid peak at 3 KeV that matched with the Ag. The synthesized CS-AgNPs exhibited substantial cytotoxicity potential against the HepG2 cells with (effective concentration (IC50) value of 15.28 µg/ml along with robust antioxidant potential, with respect to its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging (IC50 of 96.39 µg/ml) and reducing assay (IC0.5 of 209.96 µg/ml). The CS-AgNPs demonstrated encouraging antimicrobial potential against four different pathogenic bacteria and one Candida sp. with inhibition zone diameter ranged between 8.87 and 13.07 mm. Overall, the existing investigation suggested that CS-AgNPs can be an attractive, cost-effective, and environment-friendly candidate for its possible uses in the food, cosmetics, and therapeutic fields. 相似文献
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369.
Glycoproteins play important roles in various cellular events and their presence in appropriate locations in proper active
conformations is essential for many biochemical functions. Recent evidences suggest that some glycoproteins may require sorting
receptors for efficient exit from the endoplasmic reticulum. These receptors need the presence of calcium or other metal ions
for their native activity. The three-dimensional structure of such a receptor, p58/ERGIC-53, has been recently solved by x-ray
crystallography, which is a mannose-selective lectin and contains two Ca2+ ions. Homology search in the sequence databases indicates a large number of proteins which bear varying degrees of homology
in a wide spectrum of species with this receptor. In this study we have systematically searched for such genes which are potential
candidates for acting as mannose-mediated glycoprotein receptors in various species as initially inferred from their amino
acid sequence homology. Structures of a number of proteins have been predicted using knowledge-based homology modeling, and
their ability to act as the glycoprotein receptor has been explored by examining the nature of sugar-binding site. Tetramer
of mannose was docked in the binding pockets of the modeled structures followed by energy minimization and molecular dynamics
to obtain most probable structures of the complexes. Properties of these modeled complexes were studied to examine the nature
of physicochemical forces involved in the complex formation and compared with p58/ERGIC-53-mannose complex. 相似文献
370.
Day time activities are known to influence the sleep on the following night. Cyclic meditation (CM) has recurring cycles.
Previously, the low frequency (LF) power and the ratio between low frequency and high frequency (LF/HF ratio) of the heart
rate variability (HRV) decreased during and after CM but not after a comparable period of supine rest (SR). In the present
study, on thirty male volunteers, CM was practiced twice in the day and after this the HRV was recorded (1) while awake and
(2) during 6 h of sleep (based on EEG, EMG and EGG recordings). This was similarly recorded for the night’s sleep following
the day time practice of SR. Participants were randomly assigned to the two sessions and all of them practiced both CM and
SR on different days. During the night following day time CM practice there were the following changes; a decrease in heart
rate, LF power (n.u.), LF/HF ratio, and an increase in the number of pairs of Normal to Normal RR intervals differing by more
than 50 ms divided by total number of all NN intervals (pNN50) (P < 0.05, in all cases, comparing sleep following CM compared with sleep following SR). No change was seen on the night following
SR. Hence yoga practice during the day appears to shift sympatho-vagal balance in favor of parasympathetic dominance during
sleep on the following night. 相似文献