全文获取类型
收费全文 | 222篇 |
免费 | 11篇 |
国内免费 | 18篇 |
专业分类
251篇 |
出版年
2024年 | 1篇 |
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 4篇 |
2019年 | 2篇 |
2018年 | 3篇 |
2017年 | 1篇 |
2016年 | 5篇 |
2015年 | 8篇 |
2014年 | 9篇 |
2013年 | 11篇 |
2012年 | 13篇 |
2011年 | 13篇 |
2010年 | 13篇 |
2009年 | 12篇 |
2008年 | 10篇 |
2007年 | 20篇 |
2006年 | 20篇 |
2005年 | 17篇 |
2004年 | 16篇 |
2003年 | 8篇 |
2002年 | 6篇 |
2001年 | 7篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1973年 | 1篇 |
1972年 | 2篇 |
排序方式: 共有251条查询结果,搜索用时 15 毫秒
71.
毛乌素沙地南缘沙丘生物结皮中微生物分布特征 总被引:4,自引:0,他引:4
为探明半干旱沙区生物结皮中微生物分布特征,对毛乌素沙地南缘沙丘生物结皮中微生物数量进行了测定。结果表明:微生物总数从丘顶到丘间地呈递增趋势,除丘顶与迎风坡、迎风坡与背风坡结皮层微生物总数差异不显著外,其他各地貌部位结皮层微生物数量之间差异显著。同一地貌部位结皮层、0~5和5~10cm土层微生物垂直分布有变化,其变化规律为:除迎风坡放线菌数量呈先增加后递减、迎风坡微生物总数、细菌、真菌和丘顶真菌数量随剖面的加深呈递减外,其他各地貌部位微生物数量均呈先降低,后增加的趋势。微生物类群的组成表现为细菌最多,放线菌次之,真菌最少。在丘间地细菌所占微生物总数的比例与丘顶相比有所增加,而放线菌和真菌的比例有所减少。结皮下0~5和5~10cm土层微生物分布与土壤含水量的变化同步,说明土壤水分可能是影响微生物垂直分布的重要因子。 相似文献
72.
73.
74.
MARTYN C. LUCAS DAMIAN H. BUBB MIN-HO JANG KYONG HA JEROME E. G. MASTERS 《Freshwater Biology》2009,54(3):621-634
1. Conservation of freshwater animal populations requires their access to, as well as sufficient availability of, critical habitats, such as those for reproduction. Abundant small-scale barriers may cause extensive fragmentation of freshwater habitat but, by comparison to larger structures their effects are rarely considered by catchment managers. The relationship between the distribution of, and access to, spawning habitat in a regulated river, characterized by abundant small barriers, was examined for river lamprey Lampetra fluviatilis , a threatened migratory fish.
2. Telemetry of adult lamprey in the River Derwent, North East England was used to quantify upriver migration and access to spawning habitat, together with surveys of spawning habitat availability and spawning activity between 2002 and 2007.
3. Access in to the Derwent appeared severely restricted by a tidal barrage, beyond which lamprey migrated rapidly in unobstructed reaches. Of all lamprey tagged in the lower 4 km of river, or ascending the barrage, 64% and 17% passed the first and second weirs respectively, with high flows crucial for this. Although over 98% of lamprey spawning habitat occurred more than 51 km upstream, on average just 1.8% of river lamprey spawners were recorded there.
4. In order to protect or rehabilitate species or species assemblages, greater attention needs to be paid to the relative spatial distribution of low-head barriers and the resultant availability of key habitats within individual catchments. This is particularly important given the renewed emphasis internationally on low-head hydropower solutions as a source of renewable energy, and the rapid growth in numbers of low-head barriers in many catchments. 相似文献
2. Telemetry of adult lamprey in the River Derwent, North East England was used to quantify upriver migration and access to spawning habitat, together with surveys of spawning habitat availability and spawning activity between 2002 and 2007.
3. Access in to the Derwent appeared severely restricted by a tidal barrage, beyond which lamprey migrated rapidly in unobstructed reaches. Of all lamprey tagged in the lower 4 km of river, or ascending the barrage, 64% and 17% passed the first and second weirs respectively, with high flows crucial for this. Although over 98% of lamprey spawning habitat occurred more than 51 km upstream, on average just 1.8% of river lamprey spawners were recorded there.
4. In order to protect or rehabilitate species or species assemblages, greater attention needs to be paid to the relative spatial distribution of low-head barriers and the resultant availability of key habitats within individual catchments. This is particularly important given the renewed emphasis internationally on low-head hydropower solutions as a source of renewable energy, and the rapid growth in numbers of low-head barriers in many catchments. 相似文献
75.
目的:探讨原发性高血压患者热休克蛋白70(HSP-70)的表达水平,并分析其与炎症因子、血压的关系。方法:选择2017年3月至2019年3月我院收治的原发性高血压患者84例作为研究组,根据患者血压水平分为Ⅰ级组25例,Ⅱ级组37例和Ⅲ级组22例,另选同期体检健康者60例作为对照组,应用逆转录聚合酶链式反应(RT-PCR)检测各组血HSP70 m RNA水平,应用双抗体酶联免疫吸附法检测各组血清HSP-70、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和C反应蛋白(CRP)水平,比较各组血HSP70 m RNA、血清HSP70、IL-6、TNF-α和CRP水平,并分析相关性。结果:研究组血HSP-70mRNA相对表达量高于对照组(P0.05)。随高血压分级的升高各组患者血HSP-70mRNA相对表达量呈升高趋势,各组血HSP-70mRNA水平比较有统计学差异(P0.05)。研究组血清HSP-70、IL-6、TNF-α和CRP水平均高于对照组(P0.05)。随高血压分级的升高,各组患者血清HSP-70、IL-6、TNF-α和CRP水平呈升高趋势,不同组别血清HSP-70、IL-6、TNF-α和CRP水平比较有统计学差异(P0.05)。经Pearson相关分析显示,高血压患者血HSP-70 m RNA、HSP-70、IL-6、TNF-α和CRP水平与血压呈正相关(P0.05),血HSP-70 m RNA与IL-6、TNF-α和CRP水平呈正相关(P0.05),血清HSP-70与IL-6、TNF-α和CRP呈正相关(P0.05)。结论:高血压患者HSP-70和炎症因子异常升高,HSP-70与炎症因子水平和血压相关,提示HSP-70在高血压发生、发展中起到关键作用。 相似文献
76.
Chemopreventive and renal protective effects for docosahexaenoic acid (DHA): implications of CRP and lipid peroxides 总被引:2,自引:0,他引:2
Background
The fish oil-derived ω-3 fatty acids, like docosahexanoic (DHA), claim a plethora of health benefits. We currently evaluated the antitumor effects of DHA, alone or in combination with cisplatin (CP) in the EAC solid tumor mice model, and monitored concomitant changes in serum levels of C-reactive protein (CRP), lipid peroxidation (measured as malondialdehyde; MDA) and leukocytic count (LC). Further, we verified the capacity of DHA to ameliorate the lethal, CP-induced nephrotoxicity in rats and the molecular mechanisms involved therein.Results
EAC-bearing mice exhibited markedly elevated LC (2-fold), CRP (11-fold) and MDA levels (2.7-fold). DHA (125, 250 mg/kg) elicited significant, dose-dependent reductions in tumor size (38%, 79%; respectively), as well as in LC, CRP and MDA levels. These effects for CP were appreciably lower than those of DHA (250 mg/kg). Interestingly, DHA (125 mg/kg) markedly enhanced the chemopreventive effects of CP and boosted its ability to reduce serum CRP and MDA levels. Correlation studies revealed a high degree of positive association between tumor growth and each of CRP (r = 0.85) and leukocytosis (r = 0.89), thus attesting to a diagnostic/prognostic role for CRP. On the other hand, a single CP dose (10 mg/kg) induced nephrotoxicity in rats that was evidenced by proteinuria, deterioration of glomerular filtration rate (GFR, -4-fold), a rise in serum creatinine/urea levels (2–5-fold) after 4 days, and globally-induced animal fatalities after 7 days. Kidney-homogenates from CP-treated rats displayed significantly elevated MDA- and TNF-α-, but reduced GSH-, levels. Rats treated with DHA (250 mg/kg, but not 125 mg/kg) survived the lethal effects of CP, and showed a significant recovery of GFR; while their homogenates had markedly-reduced MDA- and TNF-α-, but -increased GSH-levels. Significant association was detected between creatinine level and those of MDA (r = 0.81), TNF-α ) r = 0.92) and GSH (r = -0.82); implying causal relationships.Conclusion
DHA elicited prominent chemopreventive effects on its own, and appreciably augmented those of CP as well. The extent of tumor progression in various mouse groups was highly reflected by CRP levels (thus implying a diagnostic/prognostic role for CRP). Further, this study is the first to reveal that DHA can obliterate the lethal CP-induced nephrotoxicity and renal tissue injury. At the molecular level, DHA appears to act by reducing leukocytosis, systemic inflammation, and oxidative stress. 相似文献77.
The response of chrysanthemum plants to gibberellic acid (GA3) and daminozide, grown under 6% CuSO4 and water (control) spectral filters, was evaluated to determine the involvement of gibberellins in regulation of plant height under CuSO4 filters. The CuSO4 filter increased the red (R)/farred (FR), and blue (B)/R ratio (R=600–700 nm; FR =700–800 nm; B=400–500 nm) of transmitted light. PPF under 6% CuSO4 filter was reduced by about 34% compared to PPF under water filter which averaged about 750 M·m–2·s–1. Control plants were shaded with Saran Wrap to ensure equal PPF as in the CuSO4 chamber. GA3 application increased plant height under both the control and CuSO4 filter, but the height increase under the CuSO4 filter was about 20% greater than that under the control filter. Daminozide treatment reduced plant height under the control and CuSO4 filter, but the height reduction in control plants was slightly greater than under the CuSO4 filter. The height reduction caused by daminozide was prevented by GA3 application in plants grown under the control or CuSO4 filter. The results suggest that GA3 may be partially involved in height reduction under CuSO4 filters.South Carolina Experiment Station Paper No. 3196. 相似文献
78.
以茴脑为原料,乙酸乙酯和水为新型混合溶剂,室温下通过臭氧化分解反应制取茴香醛,并对比了该体系下和传统溶剂体系下茴香醛产率的差别。实验考察了溶剂种类、溶剂用量、臭氧气流量、混合溶剂中水含量和反应时间等工艺参数,并通过红外光谱和紫外分光光度计对反应机理进行了探讨验证。该反应在水的存在下实现了室温下一锅法合成茴香醛,产率可达81.7%,避免了茴脑臭氧化物的分离及还原步骤,工艺简单,洁净环保。 相似文献
79.
CM Ward AP Wilkinson S Bramham HA Lee HW-S Chan GW Butcher A Hutchings MRA Morgan 《Mycotoxin Research》1990,6(2):73-83
From a single aflatoxin B1 oxime — bovine serum albumin conjugate, polyclonal and monoclonal antibody preparations were produced. The four rabbit polyclonal antisera were specific for aflatoxin Bi in a microtitration plate enzyme — linked immunosorbent assay. The monoclonal antibodies showed a wide range of differing specificities, recognizing, for example, aflatoxins B1, B2, G1 and G2; B1 and B2; B1 and G1; and G1 alone. No antibody preparations reacted with aflatoxin M1. The significance of these results to the strategy of anti-aflatoxin antibody production for use in quantitative enzyme immunoassays is discussed. 相似文献
80.
蒙古国喀尔喀部蒙古族4项群体指趾遗传学特征研究 总被引:1,自引:0,他引:1
在内蒙古民族大学调查了160例(男66例,女94例)来自蒙古国的喀尔喀蒙古族留学生的拇指类型、环食指长、指甲形状、足趾长4项群体指趾遗传学特征。研究结果:1)直型拇指率为44.38%,环指长率78.13%,指甲形状长型率58.75%、方型率6.88%、扁型率34.38%,足趾类型拇趾长率35.00%;2)指甲形状出现率性别间差异具统计学意义,两两特征间均未表现出明显相关关系;3)与我国10个蒙古族族群相比,多数差异具有统计学意义。 相似文献