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41.
beta-amyloid-induced migration of monocytes across human brain endothelial cells involves RAGE and PECAM-1 总被引:8,自引:0,他引:8
Giri R Shen Y Stins M Du Yan S Schmidt AM Stern D Kim KS Zlokovic B Kalra VK 《American journal of physiology. Cell physiology》2000,279(6):C1772-C1781
In patients withamyloid -related cerebrovascular disorders, e.g., Alzheimer'sdisease, one finds increased deposition of amyloid peptide (A) andincreased presence of monocyte/microglia cells in the brain. However,relatively little is known of the role of A in the trafficking ofmonocytes across the blood-brain barrier (BBB). Our studies show thatinteraction of A1-40 with monolayer of human brainendothelial cells results in augmented adhesion and transendothelialmigration of monocytic cells (THP-1 and HL-60) and peripheral bloodmonocytes. The A-mediated migration of monocytes was inhibited byantibody to A receptor (RAGE) and platelet endothelial cell adhesionmolecule (PECAM-1). Additionally, A-induced transendothelialmigration of monocytes were inhibited by protein kinase C inhibitor andaugmented by phosphatase inhibitor. We conclude that interaction ofA with RAGE expressed on brain endothelial cells initiates cellularsignaling leading to the transendothelial migration of monocytes. Wesuggest that increased diapedesis of monocytes across the BBB inresponse to A present either in the peripheral circulation or in thebrain parenchyma may play a role in the pathophysiology of A-relatedvascular disorder. 相似文献
42.
Chickpea Defensive Proteinase Inhibitors Can Be Inactivated by Podborer Gut Proteinases 总被引:4,自引:0,他引:4
Ashok P. Giri Abhay M. Harsulkar Vasanti V. Deshpande Mohini N. Sainani Vidya S. Gupta Prabhakar K. Ranjekar 《Plant physiology》1998,116(1):393-401
Developing chickpea (Cicer arietinum L.) seeds 12 to 60 d after flowering (DAF) were analyzed for proteinase inhibitor (Pi) activity. In addition, the electrophoretic profiles of trypsin inhibitor (Ti) accumulation were determined using a gel-radiographic film-contact print method. There was a progressive increase in Pi activity throughout seed development, whereas the synthesis of other proteins was low from 12 to 36 DAF and increased from 36 to 60 DAF. Seven different Ti bands were present in seeds at 36 DAF, the time of maximum podborer (Helicoverpa armigera) attack. Chickpea Pis showed differential inhibitory activity against trypsin, chymotrypsin, H. armigera gut proteinases, and bacterial proteinase(s). In vitro proteolysis of chickpea Ti-1 with various proteinases generated Ti-5 as the major fragment, whereas Ti-6 and -7 were not produced. The amount of Pi activity increased severalfold when seeds were injured by H. armigera feeding. In vitro and in vivo proteolysis of the early- and late-stage-specific Tis indicated that the chickpea Pis were prone to proteolytic digestion by H. armigera gut proteinases. These data suggest that survival of H. armigera on chickpea may result from the production of inhibitor-insensitive proteinases and by secretion of proteinases that digest chickpea Pis. 相似文献
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44.
Sudesh Raj Sharma Smith Giri Utsav Timalsina Sanjiv Sudarshan Bhandari Bikash Basyal Kusum Wagle Laxman Shrestha 《PloS one》2015,10(4)
Birth weight of a child is an important indicator of its vulnerability for childhood illness and chances of survival. A large number of infant deaths can be averted by appropriate management of low birth weight babies and prevention of factors associated with low birth weight. The prevalence of low birth weight babies in Nepal is estimated to be about 12-32%.Our study aimed at identifying major determinants of low birth weight among term babies in Nepal. A hospital-based retrospective case control study was conducted in maternity ward of Tribhuvan University Teaching Hospital from February to July 2011. A total of 155 LBW babies and 310 controls were included in the study. Mothers admitted to maternity ward during the study period were interviewed, medical records were assessed and anthropometric measurements were done. Risk factors, broadly classified into proximal and distal factors, were assessed for any association with birth of low-birth weight babies. Regression analysis revealed that a history of premature delivery (adjusted odds ratio; aOR5.24, CI 1.05-26.28), hard physical work during pregnancy (aOR1.48, CI 0.97-2.26), younger age of mother (aOR1.98, CI 1.15-3.41), mothers with haemoglobin level less than 11gm/dl (aOR0.51, CI0.24-1.07) and lack of consumption of nutritious food during pregnancy (aOR1.99, CI 1.28-3.10) were significantly associated with the birth of LBW babies. These factors should be addressed with appropriate measures so as to decrease the prevalence of low birth weight among term babies in Nepal. 相似文献
45.
Kheng-Seang Lim Ai-Huey Tan Chun-Shen Lim Kek-Heng Chua Ping-Chin Lee Norlisah Ramli Giri Shan Rajahram Fatimah Tina Hussin Kum-Thong Wong Meenakshi B. Bhattacharjee Ching-Ching Ng 《PloS one》2015,10(8)
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a rare hereditary stroke caused by mutations in NOTCH3 gene. We report the first case of CADASIL in an indigenous Rungus (Kadazan-Dusun) family in Kudat, Sabah, Malaysia confirmed by a R54C (c.160C>T, p.Arg54Cys) mutation in the NOTCH3. This mutation was previously reported in a Caucasian and two Korean cases of CADASIL. We recruited two generations of the affected Rungus family (n = 9) and found a missense mutation (c.160C>T) in exon 2 of NOTCH3 in three siblings. Two of the three siblings had severe white matter abnormalities in their brain MRI (Scheltens score 33 and 50 respectively), one of whom had a young stroke at the age of 38. The remaining sibling, however, did not show any clinical features of CADASIL and had only minimal changes in her brain MRI (Scheltens score 17). This further emphasized the phenotype variability among family members with the same mutation in CADASIL. This is the first reported family with CADASIL in Rungus subtribe of Kadazan-Dusun ethnicity with a known mutation at exon 2 of NOTCH3. The penetrance of this mutation was not complete during the course of this study. 相似文献
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47.
S. K. Sahoo S. S. Giri S. Chandra A. K. Sahu 《Zeitschrift fur angewandte Ichthyologie》2010,26(4):609-611
The aim of the experiment was to evaluate the growth and survival of Horabagrus brachysoma larvae at different stocking densities (3, 7, 13, 20, 27 and 33 larvae L?1) during their hatchery phase. Total length and wet weight of the larvae consistently decreased (P < 0.05) at the end of 14 and 28 days of rearing as the density increased. The specific growth rate was significantly (P < 0.05) highest at three larvae L?1 compared to the other five densities. The percent weight gain and survival of larvae was also highest at lowest density. The observation corroborates that catfish larvae can be reared at low densities in stagnant water conditions. Considering the value of larval growth, survival and overall weight gain, the stocking density of seven larvae L?1 has been identified as the maximum for larval rearing of H. brachysoma under hatchery conditions. 相似文献
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49.
Soma Roy Kiranmayee Rao Ch. Bhuvaneswari Archana Giri Lakshmi Narasu Mangamoori 《World journal of microbiology & biotechnology》2010,26(1):85-91
The present study describes the phytochemical profile and antimicrobial activity of Andrographis paniculata. For the present investigation, two samples of A. paniculata extracts, obtained by extraction in chloroform and chloroform + HCl, respectively, were compared for their antimicrobial
activity and further subjected to GC-MS analysis to find out the nature of the compounds responsible for the antimicrobial
activity. The antibacterial activities were assessed by measuring the diameter of the inhibition zones, MIC and MBC values.
Compared to the chloroform + HCl extract, the chloroform extract showed better antimicrobial activity against all the nine
pathogenic bacterial strains tested. The chloroform extract was observed to be active against the opportunistic and pathogenic
gram-negative bacteria, indicating its potential application related to noscomial infections. GC-MS results revealed phenols,
aromatic carboxylic acids and esters in the chloroform extract to be the molecules responsible for the antimicrobial activity
of A. paniculata. This is the first report on analysis of antimicrobial components from A. paniculata, and our results confer the utility of this plant extract in developing a novel broad spectrum antimicrobial agent. 相似文献
50.
The role of actin cytoskeleton in oscillatory fluid flow-induced signaling in MC3T3-E1 osteoblasts 总被引:1,自引:0,他引:1
Malone AM Batra NN Shivaram G Kwon RY You L Kim CH Rodriguez J Jair K Jacobs CR 《American journal of physiology. Cell physiology》2007,292(5):C1830-C1836
Fluid flow due to loading in bone is a potent mechanical signal that may play an important role in bone adaptation to its mechanical environment. Previous in vitro studies of osteoblastic cells revealed that the upregulation of cyclooxygenase-2 (COX-2) and c-fos induced by steady fluid flow depends on a change in actin polymerization dynamics and the formation of actin stress fibers. Exposing cells to dynamic oscillatory fluid flow, the temporal flow pattern that results from normal physical activity, is also known to result in increased COX-2 expression and PGE2 release. The purpose of this study was to determine whether dynamic fluid flow results in changes in actin dynamics similar to steady flow and to determine whether alterations in actin dynamics are required for PGE2 release. We found that exposure to oscillatory fluid flow did not result in the development of F-actin stress fibers in MC3T3-E1 osteoblastic cells and that inhibition of actin polymerization with cytochalasin D did not inhibit intracellular calcium mobilization or PGE2 release. In fact, PGE2 release was increased threefold in the polymerization inhibited cells and this PGE2 release was dependent on calcium release from the endoplasmic reticulum. This was in contrast to the PGE2 release that occurs in normal cells, which is independent of calcium flux from endoplasmic reticulum stores. We suggest that this increased PGE2 release involves a different molecular mechanism perhaps involving increased deformation due to the compromised cytoskeleton. mechanotransduction; cell mechanics 相似文献