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61.
62.
Nadgauda Rajani S. John C. K. Parasharami V. A. Joshi M. S. Mascarenhas A. F. 《Plant Cell, Tissue and Organ Culture》1997,48(3):181-188
Seedling explants of Bambusa arundinacea were cultured in a Murashige & Skoog (MS) based liquid medium, supplemented with
sucrose (2), coconut water (5) and 6-benzylaminopurine (2.2 μM). In 3–6 months about 70 of the cultures flowered. A comparison
was made between in vitro and in vivo flowering. Though smaller in size, in vitro florets were morphologically comparable
to the in vivo florets. Anthesis in in vivo flowering took place in the morning hours. It was more or less synchronized and
was dependent on the atmospheric temperature and humidity. The lemma and palea opened to expose both androecium and gynoecium
to the pollinating agent (wind). In in vitro flowering, some florets opened as in their in vivo counterparts, some did not
open but the anthers protruded from the tip of the partially opened lemma and palea. Anthesis was not synchronized under in
vitro conditions. Pollen fertility in in vivo and in vitro flowerings were approximately 93 and 31 respectively. Studies by
scanning electron microscopy showed some discrepancies in the pollen wall development in vitro. The trifid stigmas of in vivo
florets were highly feathery with many papillae and withered soon after pollination or within few hours. The stigmas of in
vitro developed florets were smaller with fewer and stouter papillae. They remained turgid for relatively longer periods.
Seed production in in vivo flowering was profuse whereas in in vitro flowering seeds were produced only when many florets
opened at the same time, in the same culture vessel.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
63.
Debasish Mahapatro Ramachandra Panigrahy Sudarsan Panda Rajani Kanta Mishra 《Proceedings of the Zoological Society》2018,71(3):272-280
Investigation during the period of 3 years from 2007 to 2010 on the malacofauna of Chilika lake revealed the occurrence of 126 molluscan taxa belonging to 56 families, 18 orders of three classes in the bottom sediment. Of these 61 species belonged to Bivalvia, 64 species belonged to Gastropoda and one species belonged to Polyplacophora. Maximum Bivalvia and Gastropoda taxa were found in the outer channel region of the lake. The dominating species were Crassostrea cuttackensis, Saccostrea cucullata, Brachidontes undulatus, Meretrix meretrix among bivalves and Cerethideopsilla cingulata, Bullia vittata, Nassarious stolatus, Indothias lacera, Natica tigrina, Turritella attenuata were from the gastropods. Occurrence of a large number of marine taxa is most probably associated with the opening of new lagoon during 1st August 2008. 相似文献
64.
Metabolic engineering of terpene biosynthesis in plants using a trichome‐specific transcription factor MsYABBY5 from spearmint (Mentha spicata)
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65.
Turmeric (Curcuma longa L.) is an important spice crop plant that is sterile and cannot be improved by conventional breeding. An efficient method
for stable transformation for turmeric, C. longa L., was developed using particle bombardment. Callus cultures initiated from shoots were bombarded with gold particles coated
with plasmid pAHC25 containing the bar and gusA genes each driven by the maize ubiquitin promoter. Transformants were selected on medium containing glufosinate. Transgenic
lines were established on selection medium from 50% of the bombarded calluses. Transgenic shoots regenerated from these were
multiplied and stably transformed plantlets were produced. Polymerase chain reaction (PCR) and histochemical GUS assay confirmed
the stable transformation. Transformed plantlets were resistant to glufosinate. 相似文献
66.
Volatile oil from the rhizomes of Zingiber nimmonii (J. Graham) Dalzell was isolated, characterized by analytical gas chromatography and gas chromatography-mass spectroscopy. Sixty-five constituents accounting for 97.5% of the oil were identified. Z. nimmonii rhizome oil is a unique caryophyllene-rich natural source with isomeric caryophyllenes, beta-caryophyllene (42.2%) and alpha-humulene (alpha-caryophyllene, 27.7%), as its major constituents along with traces of isocaryophyllene. The rhizome oil contained 71.2% sesquiterpenes, 14.2% oxygenated sesquiterpenes, 8.9% monoterpenes, 1.9% oxygenated monoterpenes and 1.3% non-terpenoid constituents. The antimicrobial activity of the oil was tested against human and plant pathogenic bacteria and fungi. The oil showed significant inhibitory activity against the fungi, Candida glabrata, C. albicans and Aspergillus niger and the bacteria Bacillus subtilis and Pseudomonas aeruginosa. No activity was observed against the fungus Fusarium oxysporum. 相似文献
67.
Hypertension is a major risk factor for human morbidity and mortality through its effects on target organs like heart, brain and kidneys. More intensive treatment for the effective control of blood pressure significantly reduces the morbidity and mortality. The renin angiotensin system (RAS) is a coordinated hormonal cascade of major clinical importance in the regulation of blood pressure. The principal effector peptide of RAS is angiotensin II, which acts by binding to one of the two major angiotensin II receptors AT(1) and AT(2). Angiotensin II through AT(1) receptor mediates vast majority of biologically detrimental actions. Nonpeptidic angiotensin II (AT(1)) antagonists are the most specific means to block the renin angiotensin enzymatic cascade available presently. Majority of AT(1) antagonists are based on modifications of losartan structure, the first clinically used AT(1) antagonist. In this review, a comprehensive presentation of the literature on AT(1) receptor antagonists has been given. 相似文献
68.
Anshika Srivastava Avshesh Mishra Rajan Singh Rajani Rai Neena Srivastava Balraj Mittal 《PloS one》2013,8(4)
Objective
Cholesterol gallstone disease (CGD) is a multifactorial and multistep disease. Apart from female gender and increasing age being the documented non-modifiable risk factor for gallstones the pathobiological mechanisms underlying the phenotypic expression of CGD appear to be rather complex, and one or more variations in genes could play critical roles in the diverse pathways further progressing to cholesterol crystal formation. In the present study we performed genotyping score, Multifactor dimensionality reduction (MDR) and Classification and Regression Tree analysis (CART) to identify combinations of alleles among the hormonal, hepatocanalicular transporter and adipogenesis differentiation pathway genes in modifying the risk for CGD.Design
The present case-control study recruited total of 450 subjects, including 230 CGD patients and 220 controls. We analyzed common ESR1, ESR2, PGR, ADRB3, ADRA2A, ABCG8, SLCO1B1, PPARγ2, and SREBP2 gene polymorphisms to find out combinations of genetic variants contributing to CGD risk, using multi-analytical approaches (G-score, MDR, and CART).Results
Single locus analysis by logistic regression showed association of ESR1 IVS1-397C>T (rs2234693), IVS1-351A>G (rs9340799) PGR ins/del (rs1042838) ADRB3-190 T>C (rs4994) ABCG8 D19H (rs11887534), SLCO1B1 Exon4 C>A (rs11045819) and SREBP2 1784G>C (rs2228314) with CGD risk. However, the MDR and CART analysis revealed ESR1 IVS1-397C>T (rs2234693) ADRB3-190 T>C (rs4994) and ABCG8 D19H (rs11887534) polymorphisms as the best polymorphic signature for discriminating between cases and controls. The overall odds ratio for the applied multi-analytical approaches ranged from 4.33 to 10.05 showing an incremental risk for cholesterol crystal formation. In conclusion, our muti-analytical approach suggests that, ESR1, ADRB3, in addition to ABCG8 genetic variants confer significant risk for cholesterol gallstone disease. 相似文献69.
70.
Marlena M. Westcott Maryam Ahmed Jason R. Smedberg Karishma R. Rajani Elizabeth M. Hiltbold Douglas S. Lyles 《Journal of virology》2013,87(21):11730-11740
Inhibition of host-directed gene expression by the matrix (M) protein of vesicular stomatitis virus (VSV) effectively blocks host antiviral responses, promotes virus replication, and disables the host cell. However, dendritic cells (DC) have the capacity to resist these effects and remain functional during VSV infection. Here, the mechanisms of DC resistance to M protein and their subsequent maturation were addressed. Flt3L-derived murine bone marrow dendritic cells (FDC), which phenotypically resemble resident splenic DC, continued to synthesize cellular proteins and matured during single-cycle (high-multiplicity) and multicycle (low-multiplicity) infection with VSV. Granulocyte-macrophage colony-stimulating factor (GM-CSF)-derived myeloid DC (GDC), which are susceptible to M protein effects, were nevertheless capable of maturing, but the response was delayed and occurred only during multicycle infection. FDC resistance was manifested early and was type I interferon (IFN) receptor (IFNAR) and MyD88 independent, but sustained resistance required IFNAR. MyD88-dependent signaling contributed to FDC maturation during single-cycle infection but was dispensable during multicycle infection. Similar to FDC, splenic DC were capable of maturing in vivo during the first 24 h of infection with VSV, and neither Toll-like receptor 7 (TLR7) nor MyD88 was required. We conclude that FDC resistance to M protein is controlled by an intrinsic, MyD88-independent mechanism that operates early in infection and is augmented later in infection by type I IFN. In contrast, while GDC are not intrinsically resistant, they can acquire resistance during multicycle infection. In vivo, splenic DC resist the inhibitory effects of VSV, and as in multicycle FDC infection, MyD88-independent signaling events control their maturation. 相似文献