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排序方式: 共有172条查询结果,搜索用时 31 毫秒
61.
Swati Srivastava Narender Kumar Rajani Salunke Thakur Partha Roy 《Biological trace element research》2013,152(1):135-142
In recent time, vanadium compounds are being used as antidiabetic drug and in orthopedic implants. However, the exact role of this incorporated vanadium in improving the quality of bone structure and morphology is not known. The impact of vanadium ion was studied and compared to other trace metal ions with respect to the proliferation and osteoblast differentiation of C3H10t1/2 cells. Toxicity profile of these trace metal ions revealed a descending toxicity trend of Fe2+ > Zn2+ > Cu2+ > Co2+ > Mn2+ > V5+ > Cr2+. The effect of vanadium and other trace metal ions on osteoblast differentiation was evaluated by culturing the cells for 10 days in osteoblastic medium supplemented with different trace ions at concentrations lower than their cytotoxic doses. The results indicated that vanadium has maximum impact on the induction of osteoblast differentiation by upregulating alkaline phosphatase activity and mineralization by up to 145 and 150 %, respectively (p?<?0.05), over control. Cu2+ and Zn2+ had a mild inhibitory effect, while Mn2+, Fe2+, and Co2+ demonstrated a clear decrease in osteoblast differentiation when compared to the control. The data as presented here demonstrate that orthopedic implants, if supplemented with trace metals like vanadium, may provide a source of better model for bone formation and its turnover. 相似文献
62.
Taxol protects the microtubules of concanavalin A-activated lymphocytes from disassembly by methylmercury, but DNA synthesis is still inhibited 总被引:1,自引:0,他引:1
Chitra Roy Kanteti V. S. Prasad Kenneth R. Reuhl Judy E. Little Beatrice K. Valentine David L. Brown 《Experimental cell research》1991,195(2):345-352
We have shown previously that there is a good correlation between the degree of microtubule disassembly by methylmercury (MeHg) and the extent of inhibition of DNA replication in Concanavalin A (Con A)-stimulated mouse splenic lymphocytes. The purpose of this study was to determine if these two events are causally related and to examine the effects of MeHg-induced microtubule disassembly on earlier events of the stimulation process. We show that early steps constituting the activation pathway, such as the Con A-induced increase in Ca2+ influx and the expression of interleukin 2 receptor, are not inhibited by concentrations of MeHg that disassemble microtubules. RNA synthesis is not affected by short-term (3 h) treatment with MeHg, but longer treatment (24 h) inhibits RNA synthesis. In contrast, DNA synthesis is effectively inhibited by a 3-h treatment with MeHg. In lymphocytes treated with taxol, microtubules are not disassembled by MeHg; however, the inhibition of RNA and DNA synthesis persists. We conclude that the inhibition of nucleic acid synthesis by MeHg is not causally related to MeHg-induced microtubule disassembly. 相似文献
63.
64.
Nadgauda Rajani S. John C. K. Parasharami V. A. Joshi M. S. Mascarenhas A. F. 《Plant Cell, Tissue and Organ Culture》1997,48(3):181-188
Seedling explants of Bambusa arundinacea were cultured in a Murashige & Skoog (MS) based liquid medium, supplemented with
sucrose (2), coconut water (5) and 6-benzylaminopurine (2.2 μM). In 3–6 months about 70 of the cultures flowered. A comparison
was made between in vitro and in vivo flowering. Though smaller in size, in vitro florets were morphologically comparable
to the in vivo florets. Anthesis in in vivo flowering took place in the morning hours. It was more or less synchronized and
was dependent on the atmospheric temperature and humidity. The lemma and palea opened to expose both androecium and gynoecium
to the pollinating agent (wind). In in vitro flowering, some florets opened as in their in vivo counterparts, some did not
open but the anthers protruded from the tip of the partially opened lemma and palea. Anthesis was not synchronized under in
vitro conditions. Pollen fertility in in vivo and in vitro flowerings were approximately 93 and 31 respectively. Studies by
scanning electron microscopy showed some discrepancies in the pollen wall development in vitro. The trifid stigmas of in vivo
florets were highly feathery with many papillae and withered soon after pollination or within few hours. The stigmas of in
vitro developed florets were smaller with fewer and stouter papillae. They remained turgid for relatively longer periods.
Seed production in in vivo flowering was profuse whereas in in vitro flowering seeds were produced only when many florets
opened at the same time, in the same culture vessel.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
65.
Debasish Mahapatro Ramachandra Panigrahy Sudarsan Panda Rajani Kanta Mishra 《Proceedings of the Zoological Society》2018,71(3):272-280
Investigation during the period of 3 years from 2007 to 2010 on the malacofauna of Chilika lake revealed the occurrence of 126 molluscan taxa belonging to 56 families, 18 orders of three classes in the bottom sediment. Of these 61 species belonged to Bivalvia, 64 species belonged to Gastropoda and one species belonged to Polyplacophora. Maximum Bivalvia and Gastropoda taxa were found in the outer channel region of the lake. The dominating species were Crassostrea cuttackensis, Saccostrea cucullata, Brachidontes undulatus, Meretrix meretrix among bivalves and Cerethideopsilla cingulata, Bullia vittata, Nassarious stolatus, Indothias lacera, Natica tigrina, Turritella attenuata were from the gastropods. Occurrence of a large number of marine taxa is most probably associated with the opening of new lagoon during 1st August 2008. 相似文献
66.
Metabolic engineering of terpene biosynthesis in plants using a trichome‐specific transcription factor MsYABBY5 from spearmint (Mentha spicata) 下载免费PDF全文
67.
Turmeric (Curcuma longa L.) is an important spice crop plant that is sterile and cannot be improved by conventional breeding. An efficient method
for stable transformation for turmeric, C. longa L., was developed using particle bombardment. Callus cultures initiated from shoots were bombarded with gold particles coated
with plasmid pAHC25 containing the bar and gusA genes each driven by the maize ubiquitin promoter. Transformants were selected on medium containing glufosinate. Transgenic
lines were established on selection medium from 50% of the bombarded calluses. Transgenic shoots regenerated from these were
multiplied and stably transformed plantlets were produced. Polymerase chain reaction (PCR) and histochemical GUS assay confirmed
the stable transformation. Transformed plantlets were resistant to glufosinate. 相似文献
68.
Volatile oil from the rhizomes of Zingiber nimmonii (J. Graham) Dalzell was isolated, characterized by analytical gas chromatography and gas chromatography-mass spectroscopy. Sixty-five constituents accounting for 97.5% of the oil were identified. Z. nimmonii rhizome oil is a unique caryophyllene-rich natural source with isomeric caryophyllenes, beta-caryophyllene (42.2%) and alpha-humulene (alpha-caryophyllene, 27.7%), as its major constituents along with traces of isocaryophyllene. The rhizome oil contained 71.2% sesquiterpenes, 14.2% oxygenated sesquiterpenes, 8.9% monoterpenes, 1.9% oxygenated monoterpenes and 1.3% non-terpenoid constituents. The antimicrobial activity of the oil was tested against human and plant pathogenic bacteria and fungi. The oil showed significant inhibitory activity against the fungi, Candida glabrata, C. albicans and Aspergillus niger and the bacteria Bacillus subtilis and Pseudomonas aeruginosa. No activity was observed against the fungus Fusarium oxysporum. 相似文献
69.
Hypertension is a major risk factor for human morbidity and mortality through its effects on target organs like heart, brain and kidneys. More intensive treatment for the effective control of blood pressure significantly reduces the morbidity and mortality. The renin angiotensin system (RAS) is a coordinated hormonal cascade of major clinical importance in the regulation of blood pressure. The principal effector peptide of RAS is angiotensin II, which acts by binding to one of the two major angiotensin II receptors AT(1) and AT(2). Angiotensin II through AT(1) receptor mediates vast majority of biologically detrimental actions. Nonpeptidic angiotensin II (AT(1)) antagonists are the most specific means to block the renin angiotensin enzymatic cascade available presently. Majority of AT(1) antagonists are based on modifications of losartan structure, the first clinically used AT(1) antagonist. In this review, a comprehensive presentation of the literature on AT(1) receptor antagonists has been given. 相似文献
70.
Anshika Srivastava Avshesh Mishra Rajan Singh Rajani Rai Neena Srivastava Balraj Mittal 《PloS one》2013,8(4)