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51.
Alba G El Bekay R Alvarez-Maqueda M Chacón P Vega A Monteseirín J Santa María C Pintado E Bedoya FJ Bartrons R Sobrino F 《FEBS letters》2004,573(1-3):219-225
In the present study, we have examined the potential ability of 5'-AMP-activated protein kinase (AMPK) to modulate NADPH oxidase activity in human neutrophils. AMPK activated with either 5'-aminoimidazole-4-carboxamide ribonucleoside (AICAR) or with 5'-AMP significantly attenuated both phorbol 12-myristate 13-acetate (PMA) and formyl methionyl leucyl phenylalanine-stimulated superoxide anion O2- release by human neutrophils, consistently with a reduced translocation to the cell membrane and phosphorylation of a cytosolic component of NADPH oxidase, namely p47phox. AMPK was found to be present in human neutrophils and to become phosphorylated in response to either AICAR or other stimulators of its enzyme activity. Furthermore, AICAR also strongly reduced PMA-dependent H2O2 release, and induced the phosphorylation of c-jun N-terminal kinase 1 (p46), p38 mitogen-activated protein kinase and extracellular signal-regulated kinase. Present data demonstrate for the first time that the activation of AMPK, in states of low cellular energy charge (such as under high levels of 5'-AMP) or other signals, could be a factor contributing to reduce the host defense mechanisms. 相似文献
52.
N Nuwayri-Salti K Knio A Jammoul R Fakhoury KA Sarhane H Nakkash-Chmaisse 《PLoS neglected tropical diseases》2012,6(8):e1782
Background
Systemic leishmaniasis has been known to present with prolonged fever, hepatosplenomegaly and wasting. Beside this classical form, a sub-clinical form has been identified. It is described with either one or two of the above symptoms missing; other findings have been reported instead, such as lymphadenopathy and anemia. In this report, we reveal a third unsuspected form which we are referring to as “atypical”.Methodology/Principal Findings
Patients suspected to be immune-deficient were referred to our immunology specialized laboratory to study some aspects of their immune functions (not normally covered in the general laboratory). Multiple specialized tests were performed, including microscopic examinations using appropriate stains, and mainly cultures of biopsies on several types of specialized media. 19·4% of 160 patients were found to have close to normal laboratory profiles, but exhibited dysfunctional macrophages laden with Leishmania parasites.Conclusions/Significance
Findings such as the ones we obtained allowed us to uncover the presence of patients with an atypical form of systemic leishmaniasis. It presents with symptoms masquarading a condition in which the immune system is non functional. This predisposes patients to recurrent secondary infections resulting in clinical pictures with a great variety of signs and symptoms. These findings alerted us to the fact that systemic leishmaniasis presents with a much wider spectrum of signs and symptoms than so far suspected and is far more common than diagnosed to date. Furthermore, among these 31 patients was a number of adults. This proved that in our area systemic leishmaniasis is surely not limited to the pediatric age group. Our recommendation is to entertain the diagnosis of atypical systemic leishmaniasis in any patient with an unexplained depressed immunity state and in whom no obvious immunologic defect can be identified. 相似文献53.
Julián Salas Mercedes Montiel Eugenio Jiménez Miguel Valenzuela José Francisco Valderrama Rafael Castillo Sergio González Rajaa El Bekay 《Cell and tissue research》2009,338(2):313-318
The endogenous proangiogenic properties of adipose tissue are well recognized. Although the adult human thymus has long been
known to degenerate into fat tissue, it has never been considered as a potential source of angiogenic factors. We have investigated
the expression of diverse angiogenic factors, including vascular endothelial growth factor A and B, angiopoietin 1, and tyrosine-protein
kinase receptor-2 (an angiopoietin receptor), and then analyzed their physiological role on endothelial cell migration and
proliferation, two relevant events in angiogenesis. The detection of the gene and protein expression of the various proteins
has been performed by immunohistochemistry, Western blotting, and quantitative real-time polymerase chain reaction. We show,
for the first time, that adult thymus fat produces a variety of angiogenic factors and induces the proliferation and migration
of human umbilical cord endothelial cells. Based on these findings, we suggest that this fat has a potential angiogenic function
that might affect thymic function and ongoing adipogenesis within the thymus. 相似文献
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