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291.
In general, enveloped viruses use two different entry strategies and are classified accordingly into pH-dependent and pH-independent viruses. Different members of the retrovirus family use one or the other strategy. Little is known about the uptake of foamy viruses (FV), a special group of retroviruses, into the target cells. In this study, we examined the pH dependence of FV entry by analyzing FV envelope glycoprotein (Env)-mediated infection of target cells with murine leukemia virus or FV vector pseudotypes in the presence of various lysosomotropic agents. Similar to vesicular stomatitis virus glycoprotein G (VSV-G)-mediated uptake, FV Env-mediated entry was inhibited by various lysosomotropic agents, suggesting a pH-dependent endocytic pathway. However, in contrast to its effect on VSV-G pseudotypes, chloroquine failed to reduce the infectivity of FV Env pseudotypes, implying that the pathway is different from that of VSV-G. Glycoproteins of various other FV species showed inhibition profiles similar to that of the prototype FV (PFV) Env. Analysis of the pH dependence of the FV Env-mediated fusion process in a cell-to-cell fusion assay revealed an induction of syncytium formation by a short exposure to acidic pH, peaking around pH 5.5. Interestingly, of all FV Env species analyzed, only the PFV Env had a significant fusion activity at neutral pH. Taken together, these data suggest a pH-dependent endocytic pathway for infection of target cells by FV. 相似文献
292.
Feline foamy virus genome and replication strategy 总被引:1,自引:0,他引:1
Roy J Rudolph W Juretzek T Gärtner K Bock M Herchenröder O Lindemann D Heinkelein M Rethwilm A 《Journal of virology》2003,77(21):11324-11331
Crucial aspects of the foamy virus (FV) replication strategy have so far only been investigated for the prototypic FV (PFV) isolate, which is supposed to be derived from nonhuman primates. To study whether the unusual features of this replication pathway also apply to more-distantly related FVs, we constructed feline FV (FFV) infectious molecular clones and vectors. It is shown by quantitative RNA and DNA PCR analysis that FFV virions contain more RNA than DNA. Full-length linear DNA was found in extracellular FFV by Southern blot analysis. Similar to PFV, azidothymidine inhibition experiments and the transfection of nucleic acids extracted from extracellular FFV indicated that DNA is the functional relevant FFV genome. Unlike PFV, no evidence was found indicating that FFV recycles its DNA into the nucleus. 相似文献
293.
294.
Cloning and characterization of human erythroid membrane-associated protein, human ERMAP 总被引:5,自引:0,他引:5
Xu H Foltz L Sha Y Madlansacay MR Cain C Lindemann G Vargas J Nagy D Harriman B Mahoney W Schueler PA 《Genomics》2001,76(1-3):2-4
We describe here the cloning and characterization of the human gene ERMAP, identified by subtractive hybridization using early and late gestation human fetal liver. By in situ hybridization, we found human ERMAP to be expressed not only in erythoid cells in fetal liver and adult bone marrow, but also in reticulocytes and circulating erythroblasts in 8-12-week fetal cord blood. The human ERMAP protein is predicted to contain a transmembrane segment and one extracellular immunoglobulin fold (IgV). The cytoplasmic region contains a highly conserved B30.2 motif, multiple consensus sequences for kinases, and post-Golgi sorting signals. The protein was localized to the cell surface as shown by an antibody specific for a peptide predicted from the IgV fold. The amino acid sequence of human ERMAP is highly homologous with that of mouse ERMAP, but differs in the number of extracellular immunoglobulin folds. Human ERMAP represents a new unique member of the rapidly growing B30.2 domain proteins. 相似文献
295.
Lindemann B 《The Journal of membrane biology》2001,184(3):203-210
The article describes work and impact of Hans H. Ussing, a founder of epithelial physiology. Emphasis is on Ussing's model of epithelial transport, which showed early how a complex function can arise from a few basic principles. The KJU-model was developed 1958 for the amphibian epidermis and later applied and adapted to many epithelia, but especially to those that express amiloride-sensitive sodium channels in their apical membrane. Some of the subsequent research dealing with such channels and their cellular environment is briefly reviewed. The ideas of Hans Ussing were and are an inspiration to many of us, who continue to work in the way Ussing has taught us. 相似文献
296.
297.
Measurement of the force produced by an intact bull sperm flagellum in isometric arrest and estimation of the dynein stall force 下载免费PDF全文
The force generated by a detergent-extracted reactivated bull sperm flagellum during an isometric stall was measured with a force-calibrated glass microprobe. The average isometric stall force from 48 individual measurements was 2.5 +/- 0.7 x 10(-5) dyne (2.5 +/- 0.7 x 10(-10) N). The force measurements were obtained by positioning a calibrated microprobe in the beat path of sperm cells that were stuck by their heads to a glass microscope slide. The average position of the contact point of the flagellum with the probe was 15 microm from the head-tail junction. This average lever arm length multiplied by the measured force yields an estimate of the active bending moment (torque) of 3.9 x 10(-8) dyne x cm (3.9 x 10(-15) N x m). The force was sustained and was for the most part uniform, despite the fact that the flagellum beyond the point of contact with the probe usually continued beating. It appears that the dynein motors in the basal portion of the flagellum continue to pull in an isometric stall for as long as the motion of the flagellum is blocked. If dynein motors in the flagellum distal to the contact point with the probe were contributing force to the displacement of the probe, then the flagellar segment immediately past the point of contact would have to show a net curvature in the direction of the probe displacement. No such curvature bias was observed in the R-bend arrests, and only a small positive curvature bias was measured in the P-bend arrests. Our analysis of the data suggests that more than 90% of the sustained force component is generated by the part of the flagellum between the probe and the flagellar base. Based on this premise, the isometric stall force per dynein head is estimated to be 5.0 x 10(-7) dyne (5 pN). This equals approximately 1.0 x 10(-6) dyne (10 pN) per intact dynein arm. These values are close to the isometric stall force of isolated dynein. This suggests that all of the dynein heads between the base and the probe, on the active side of the axoneme, are contributing to the force exerted against the probe. 相似文献
298.
299.
Denk A Goebeler M Schmid S Berberich I Ritz O Lindemann D Ludwig S Wirth T 《The Journal of biological chemistry》2001,276(30):28451-28458
300.
Lesich KA Kelsch CB Ponichter KL Dionne BJ Dang L Lindemann CB 《Biology of reproduction》2012,86(4):105
Triton X-100-extracted mouse sperm treated with 0.1 mM ATP and 1.0 mM Ca(2+) exhibit an extremely coiled configuration that has been previously described as a curlicue. Sperm in the curlicue configuration exhibit a monotonically curved flagellum where the shear angle of the flagellum can reach a value as high as 14 radians at the flagellar tip. We utilized this strong reaction to Ca(2+) to elucidate the mechanism of the calcium response. The disintegration of the axoneme was facilitated by the use of an extraction procedure that removed the mitochondrial sheath without eliminating the calcium response. The order of emergence of the doublet microtubule outer dense fiber complexes was observed in the presence and absence of added Ca(2+). The identity of the emergent elements was confirmed by transmission electron microscopy. Ca(2+) altered the order of emergence of internal axoneme elements to favor the appearance of the elements of the 9-1-2 side of the axoneme. These elements are propelled baseward by the action of dyneins on doublets 1 and 2. It was also possible to establish that the motive force for maintaining the curlicue configuration is dynein-based. The curlicues were relaxed by inhibition with 50 μM NaVO(3) and were reestablished by disinhibiting the vanadate with 2.5 mM catechol. 相似文献