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排序方式: 共有205条查询结果,搜索用时 125 毫秒
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J Wittmann W Kugler H Rahn 《Comparative biochemistry and physiology. A, Comparative physiology》1984,77(3):547-551
Treating chick embryos with high doses of thiourea (32.8 mumol) on day 17 of incubation resulted in prevention of hatching and of active breathing. Furthermore, thiourea also prevented the increase of O2 consumption and the marked increase of somatic activity associated with the final hatching act. These findings provide evidence for the importance of active breathing in the prenatal period to initiate pipping and hatching of the avian embryo. 相似文献
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Zusammenfassung Bei vier Personen, zwei männlichen und zwei weiblichen, konnte eine Emanation aus den Fingerspitzen festgestellt werden, welche eine bestimmte Hefenart (Saccharomyces Mycoderma punctisporus Guill.) in kurzer Zeit tötete.Bei allen vier Personen konnte diese Strahlung auf pathologische Zustände zurückgeführt werden.Die Strahlung ging nicht durch Glas, wohl aber durch Quarz und ist daher sehr wahrscheinlich ultraviolett.Chemisch hergestelltes Oxycholesterin verursacht in Emulsion mit Wasser ebenfalls Tötung der Hefe durch Quarz hindurch. Oxycholesterin ist ein Bestandteil des aus den Talgdrüsen ausgeschiedenen Fettes.Der Snyder Research Foundation, durch deren finanzielle Unterstützung diese Arbeit ermöglicht wurde, sei auch an dieser Stelle bestens gedankt. 相似文献
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A rapid, sensitive and automated method for detection of Salmonella species in foods using AG-9600 AmpliSensor Analyzer 总被引:5,自引:0,他引:5
S. Chen A. Yee M. Griffiths K.Y. Wu C.-N. Wang K. Rahn & S.A. De Grandis 《Journal of applied microbiology》1997,83(3):314-321
The AG-9600 AmpliSensor Analyzer is an automated fluorescence-based system for detection of polymerase chain reaction (PCR) products. The principle of the AmpliSensor PCR assay involves amplification-mediated disruption of a fluorogenic DNA signal duplex (AmpliSensor) that is homologous to a target sequence within a 284-bp amplified fragment of the Salmonella invA gene. Since the assay is homogenous, the data can be obtained by direct measurement of fluorescence of the amplification mixture. The accumulation of the amplified product, reflected by the fluorescence index, is monitored cycle by cycle by the AG-9600 Analyzer. The detection limit of the assay was less than 2 colony-forming units (cfu) per PCR reaction using a pure culture of Salmonella typhimurium. In post-spiking experiments in which Salmonella was added to the overnight pre-enriched samples (chicken carcass rinses, ground beef, ground pork and raw milk), the detection limit of the assay was 2–6 cfu per PCR reaction. In pre-spiking experiments in which Salmonella was added to the samples prior to overnight pre-enrichment, the detection limit was less than 3 cfu per 25 g or 25 ml of food. The assay was up to 2 orders of magnitude more sensitive than detection by ethidium bromide-stained agarose gel electrophoresis. To further evaluate assay performance, 54 naturally contaminated chicken carcass rinses, 65 raw milk and six ground pork samples were tested in the study. Thirty-eight Salmonella- positive samples confirmed by the Modified Semi-solid Rappaport-Vassiliadis (MSRV) culture assay were found positive using the AmpliSensor assay. Two chicken carcass rinses found positive using the assay were MSRV-negative. In addition, relative quantification using the AmpliSensor assay was linear up to 3 logs of initial target concentration in artificially contaminated food samples. 相似文献
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Altered protein expression pattern in colon tissue of mice upon supplementation with distinct selenium compounds
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Jette Rahn Claudia Lennicke Anna P. Kipp Andreas S. Müller Ludger A. Wessjohann Rudolf Lichtenfels Barbara Seliger 《Proteomics》2017,17(11)
The essential trace element selenium (Se) is controversially discussed concerning its role in health and disease. Its various physiological functions are largely mediated by Se incorporation in the catalytic center of selenoproteins. In order to gain insights into the impact of Se deficiency and of supplementation with different Se compounds (selenite, selenate, selenomethionine) at defined concentrations (recommended, 150 μg/kg diet; excessive, 750 μg/kg diet) in murine colon tissues, a 20‐week feeding experiment was performed followed by analysis of the protein expression pattern of colon tissue specimens by 2D‐DIGE and MALDI‐TOF MS. Using this approach, 24 protein spots were identified to be significantly regulated by the different Se compounds. These included the antioxidant enzyme peroxiredoxin‐5 (PRDX5), proteins with binding capabilities, such as cofilin‐1 (COF1), calmodulin, and annexin A2 (ANXA2), and proteins involved in catalytic processes, such as 6‐phosphogluconate dehydrogenase (6PGD). Furthermore, the Se compounds demonstrated a differential impact on the expression of the identified proteins. Selected target structures were validated by qPCR and Western blot which mainly confirmed the proteomic profiling data. Thus, novel Se‐regulated proteins in colon tissues have been identified, which expand our understanding of the physiologic role of Se in colon tissue. 相似文献
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Eduardo N Taboada Joanne M MacKinnon Christian C Luebbert Victor PJ Gannon John HE Nash Kris Rahn 《BMC evolutionary biology》2008,8(1):229