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961.
962.
Louis J. Taylor Marisol I. Dothard Meagan A. Rubel Audrey A. Allen Young Hwang Aoife M. Roche Jevon Graham-Wooten Ayannah S. Fitzgerald Layla A. Khatib Alessia Ranciaro Simon R. Thompson William R. Beggs Michael C. Campbell Gaonyadiwe G. Mokone Sununguko Wata Mpoloka Charles Fokunang Alfred K. Njamnshi Eric Mbunwe Dawit Woldemeskel Gurja Belay Thomas Nyambo Sarah A. Tishkoff Ronald G. Collman Frederic D. Bushman 《Journal of virology》2021,95(21)
963.
J C Medina D Roche B Shan R M Learned W P Frankmoelle D L Clark T Rosen J C Jaen 《Bioorganic & medicinal chemistry letters》1999,9(13):1843-1846
In this report, we describe the synthesis of halogenated benzenesulfonamide compounds and their ability to inhibit the growth of HeLa, MCF-7 and MCF-7/ADR tumor cells in vitro. The multidrug resistance (MDR) phenotype of certain cells does not affect their sensitivity to these compounds. These agents belong to a family of compounds previously shown to bind irreversibly to cysteine-239 of beta-tubulin. Consistent with this mechanism of action, the cytotoxicities of these compounds appear to correlate with their ability to undergo nucleophilic aromatic substitution. 相似文献
964.
The sugar binding activity of MR60, a mannose-specific shuttling lectin, requires a dimeric state 总被引:1,自引:0,他引:1
MR60 is an intracellular membrane protein which has been shown to act as a
mannoside specific lectin and to be identical to ERGIC-53, a protein
characteristic of the endoplasmic reticulum-Golgi apparatus- intermediate
compartment, acting as a shuttle. According to its primary sequence, this
MR60/ERGIC-53 protein contains a luminal domain including the carbohydrate
recognition domain, a stem, a transmembrane segment and a cytosolic domain.
The endogenous MR60/ERGIC-53 protein is spontaneously oligomeric, (dimers
and hexamers). In this paper, we study the relationship between the
oligomerization state and the sugar binding capacity by using recombinant
proteins. The expression of the recombinant proteins was evidenced by
immunocytochemistry and by immunoprecipitation followed by SDS-PAGE
analysis. The full size recombinant protein binds mannosides and is
oligomeric, up to the hexameric form. Two truncated proteins lacking the
transmembrane and the cytosolic domains were prepared and characterized. A
long one, containing the cysteine 466 close to the C-terminal end of the
recombinant protein but lacking the cysteine 475, close to the C- terminal
end of the native protein, does bind mannosides and forms dimers but no
higher oligomeric forms. A shorter one, lacking both the cysteines 466 and
475, does not bind mannosides and does not form dimers or higher polymers.
The two cysteines in the carbohydrate recognition domain (C190 and C230)
are not involved in the stabilization of oligomers. In conclusion, this
study shows that the luminal moiety of MR60/ERGIC-53 contains a device
allowing both its oligomeric pattern and its sugar binding capability.
相似文献
965.
The adhesive interactions of nerve growth cones stabilize elongating nerve fibers and mediate transmembrane signaling to regulate growth cone behaviors. We used interference reflection microscopy and immunocytochemistry to examine the dynamics and composition of substratum contacts that growth cones of chick sensory neurons make with extracellular adhesive glycoproteins, fibronectin and laminin. Interference reflection microscopy indicated that sensory neuronal growth cones on fibronectin-treated substrata, but not on laminin, make contacts that have the appearance and immobility of fibroblastic focal contacts. Interference reflection microscopy and subsequent immunocytochemical staining showed that β1 integrin and phosphotyrosine residues were concentrated at growth cone sites that resemble focal contacts. Two other components of focal contacts, paxillin and zyxin, were also co-localized with concentrated phosphotyrosine residues at sites that resemble focal contacts. Such staining patterns were not observed on laminin-treated substrata. Growth cone migration on fibronectin-treated substrata was inhibited by herbimycin A, a tyrosine kinase inhibitor. We conclude that sensory neuronal growth cones distinguish fibronectin from laminin by making contacts with distinct organization and regulation of cytoskeletal components at the adhesive sites. This finding suggests that growth cone interactions with different adhesive molecules lead to distinctive transmembrane organization and signaling to regulate nerve fiber elongation. © 1996 John Wiley & Sons, Inc. 相似文献
966.
A study of a stocked upland reservoir trout fishery, using gill nets, revealed the presence of a large stock of pike and poor
trout numbers. The overall growth rate of pike was slow. Two year-classes of pike dominated, but the broad age and length
frequency ranges, and the high average weight of individuals were indicative of an unexploited stock. Higher than normal water
levels during the spawning period and into the summer period in both 1985 and 1986, coincided with the dominant pike year-classes
recorded several years later.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
967.
H.G.F. Richter C. Freichel J. Huwyler T. Nakagawa M. Nettekoven J.-M. Plancher S. Raab O. Roche F. Schuler S. Taylor C. Ullmer R. Wiegand 《Bioorganic & medicinal chemistry letters》2010,20(19):5713-5717
A novel series of potent histamine H3 receptor inverse agonists based on the 3,4-dihydro-2H-pyrazino[1,2-a]indol-1-one scaffold has been discovered. Several compounds display high selectivity over other histamine receptor subtypes and have favorable physicochemical properties, low potential for CYP450 enzyme inhibition and high metabolic stability in microsomal preparations. (R)-2-Cyclopropylmethyl-8-(1-isopropyl-piperidin-4-yloxy)-3-methyl-3,4-dihydro-2H-pyrazino[1,2-a]indol-1-one (8t) showed good in vivo efficacy after per os application in an acute rat dipsogenia model of water intake. 相似文献
968.
Repeated measures of periosteal and endosteal widths of the left second metacarpal from two samples of radiographs were made independently with either a dial caliper or an electronic digitizer connected to a microcomputer. In addition, periosteal and endosteal bone widths of a common sample of radiographs were measured with dial calipers and the electronic digitizer. Dial calipers and an electronic digitizer are both very reliable, but intraobserver errors produced with the use of the digitizer are significantly less than those produced with the use of dial calipers. However, measurements made with the electronic digitizer are systematically larger than corresponding widths measured with dial calipers, but this difference is very small. 相似文献
969.
Ultrasonic and skinfold caliper measures of subcutaneous adipose tissue thickness in elderly men and women 总被引:1,自引:0,他引:1
Ultrasonic and skinfold caliper measures of subcutaneous adipose tissue were taken in a sample of elderly men and women, 65 to 99 years of age with an A-mode Ithaco Body Composition Meter and a Holtain skinfold caliper. For most of the measurements, standard deviations for the ultrasonic measurements were three or more times larger than corresponding caliper measurements. Also, mean absolute interobserver errors for the ultrasonic measurements were considerably larger than those for skinfold calipers. At best, the interobserver reliability for the ultrasound machine was 68% while the poorest reliability for the skinfold caliper was 88%. In the form tested, the Ithaco Body Composition Meter is not as accurate for measuring subcutaneous adipose tissue thickness in the elderly as a Holtain skinfold caliper. 相似文献
970.
Further characterization of the platinum-reactive component of the alpha 2-macroglobulin-receptor recognition site. 总被引:3,自引:1,他引:2 下载免费PDF全文
alpha 2-Macroglobulin (alpha 2M)-methylamine that had been allowed to react with cis-dichlorodiammineplatinum(II) (cis-DDP) bound with greatly reduced affinity to specific alpha 2M receptors, as determined by macrophage binding studies in vitro and plasma-clearance experiments in vivo. Subsequent reaction with diethyl dithiocarbamate completely restored receptor recognition function. The optimal effect was obtained when the diethyl dithiocarbamate concentration was twice the total platinum concentration. alpha 2M-methylamine that was allowed to react with H2O2 competed less effectively for specific cell-surface binding sites, as demonstrated by studies both in vivo and in vitro. The apparent dissociation constant was increased nearly 7-fold by a 15 min exposure to H2O2. alpha 2M-methylamine was affected significantly less by the H2O2 exposure after pretreatment with cis-DDP. Amino acid analysis indicated that H2O2 treatment of alpha 2M modified 19 of the 25 methionine residues per alpha 2M subunit. Pretreatment with cis-DDP protected two to four of these methionine residues. The only other residue altered by H2O2 treatment of alpha 2M was histidine. A net decrease of two histidine residues per subunit was observed, but cis-DDP pretreatment did not alter this result. In order to rule out the slight possibility that histidine modification might account for the observed H2O2-induced loss in receptor recognition, diethyl pyrocarbonate was employed as a histidine-modifying reagent. This treatment modified 53 histidine residues in both native and fast-form alpha 2M. Fast-form alpha 2M was still recognized by the alpha 2M receptor, as determined by studies both in vivo and in vitro; however, a fraction of the modified protein now cleared via the acyl-low-density-lipoprotein receptor as well. Reaction of diethyl pyrocarbonate-treated alpha 2M with hydroxylamine reversed derivatization of 43 of the 53 histidine residues. Moreover, this treatment also resulted in an alpha 2M fast-form preparation that was recognized only by the alpha 2M receptor. It is concluded that cis-DDP and H2O2 modify a critical methionine residue in the primary sequence of the alpha 2M-receptor recognition site. 相似文献