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111.
Ghanaim Amira Mohamed Foaad Mohammed Abdulaziz Gomaa Eman Zakaria Dougdoug Khalid Abdelfatah El Mohamed Gamal Eldidamony Arisha Ahmed Hamed Khamis Tarek 《International microbiology》2023,26(2):343-359
International Microbiology - Diabetic foot ulcer (DFU) represented the most feared diabetic complication that caused the hospitalization of the diabetic patient. DFU was usually characterized with... 相似文献
112.
Zakaria A. M. Baka 《Mycopathologia》1992,120(2):103-111
The ultrastructure of the uredinial stage of the rust fungus,Puccinia polypogonis onPolypogon monspeliensis is described, using scanning and transmission electron microscopy. This study examined the urediniospores, intercellular hyphae, and haustoria of the fungus. The formation and structure of urediniospores is similar to those of otherPuccinia species. The ultrastructure of intercellular hyphae and haustoria is similar to those of other rust fungi, but with some differences. No modifications are observed in the wall of the haustorial mother cells during penetration. A collar is found only around old haustoria. In most cases, one nucleus is detected inside the haustorial body and no nucleoli are seen in the nuclei of intercellular hyphae and haustoria. The host-parasite interface, including extrahaustorial matrix and extrahaustorial membrane, is also discussed and compared with those of other rust fungi. 相似文献
113.
Lipopolysaccharide (LPS) signaling leading to nuclear factor-kappaB activation in mononuclear phagocytes involves interleukin-1 receptor-associated kinase (IRAK), which is rapidly activated after exposure to agonist. Although it is known that IRAK also undergoes rapid inactivation/degradation in response to LPS, providing negative feedback leading to LPS tolerance, mechanisms governing IRAK degradation are not fully understood. In the present study, examination of LPS signaling showed that IRAK degradation was bimodal and involved dual receptors and distinct pathways. Rapid degradation of IRAK, occurring within 30 min of exposure to agonist, was shown to signal through CD14/TLR4 and was regulated by phosphatidylinositol 3-kinase. A second delayed wave of IRAK degradation occurred 2 h after exposure to LPS and was mediated by CR3 independently of phosphatidylinositol 3-kinase. Thus, multiple independent mechanisms have evolved to regulate IRAK degradation, likely reflecting the importance of limiting cellular responses to LPS. Recognition of a CR3-dependent, CD14/TLR4-independent pathway leading to IRAK degradation has implications for understanding modulation of LPS responses by cells with important immunoregulatory function such as dendritic cells that are CD14(-). 相似文献
114.
Synthesis of half-mustard combi-molecules with fluorescence properties: correlation with EGFR status
Rachid Z Brahimi F Domarkas J Jean-Claude BJ 《Bioorganic & medicinal chemistry letters》2005,15(4):1135-1138
The synthesis of 6-(2-chloroethylamino)-4-anilinoquinazolines ZR2002 and ZR2003 designed to block EGFR tyrosine kinase and to damage genomic DNA is described. These compounds present fluorescence properties that permitted the quantitation of their subcellular uptake by flow cytometry. Fluorescence intensities increased with increasing levels of EGFR in a panel of isogenic and established cell lines. 相似文献
115.
M. Mafizur Rahman Kwang Bae Yoon Ji Young Kim Mohamed Zakaria Hussin 《Animal cells and systems.》2016,20(2):86-94
We characterized the complete mitogenome of Pipistrellus coromandra (Indian pipistrelle) for comparative analysis of mitogenomes and for resolving the phylogenetic relationship of four tribes in the subfamily Vespertilioninae. The mitogenome size of P. coromandra was 17,153?bp, with a control region and a typical set of 37 mitochondrial genes. The nucleotide composition of the P. coromandra mitogenome showed an AT bias with a nucleotide composition of 33.5% A, 30.7% T, 13.3% G, and 22.5% C. The mitochondrial protein-coding genes in P. coromandra use the standard start codon (ATN), two stop codons (TAA and AGA), and two incomplete stop codons (TA- and T--). The intertribal relationship of four tribes was highly resolved from the phylogenetic analysis of mitogenome sequences. 相似文献
116.
Sheikh Imranudin Sheikh-Ali Akil Ahmad Siti-Hamidah Mohd-Setapar Zainul Akmal Zakaria Norfahana Abdul-Talib Aidee Kamal Khamis Md Enamul Hoque 《Journal of microbiology (Seoul, Korea)》2014,52(10):807-818
The contamination of food and feed by Aspergillus has become a global issue with a significant worldwide economic impact. The growth of Aspergillus is unfavourable to the development of food and feed industries, where the problems happen mostly due to the presence of mycotoxins, which is a toxic metabolite secreted by most Aspergillus groups. Moreover, fungi can produce spores that cause diseases, such as allergies and asthma, especially to human beings. High temperature, high moisture, retarded crops, and poor food storage conditions encourage the growth of mold, as well as the development of mycotoxins. A variety of chemical, biological, and physical strategies have been developed to control the production of mycotoxins. A biological approach, using a mixed culture comprised of Saccharomyces cerevisiae and Lactobacillus rhamnosus resulted in the inhibition of the growth of fungi when inoculated into fermented food. The results reveal that the mixed culture has a higher potential (37.08%) to inhibit the growth of Aspergillus flavus (producer of Aflatoxin) compared to either single culture, L. rhamnosus NRRL B-442 and S. cerevisiae, which inhibit the growth by 63.07% and 64.24%, respectively. 相似文献
117.
Vijayendran Govindasamy Veronica Sainik Ronald Swapnil Totey Salina Binti Din Wan Mahadzir Bin Wan Mustafa Satish Totey Zubaidah Zakaria Ramesh R. Bhonde 《In vitro cellular & developmental biology. Animal》2010,46(9):764-773
Stem cells isolated from dental pulp possess the capacity for self-renewal and the potential for multi-lineage differentiation.
However, dental pulp stem cells have different characteristics in terms of their culture conditions. The success of stem cells
culture is governed by its micro-environmental niche. Therefore, we studied the effects of culture niche on long-term expansion
of dental pulp stem cells in terms of cell morphology, growth kinetics, senescence pattern, cell surface marker expression
differentiation capacity, and seeding plating density of dental pulp stem cells in four different, widely used media composition
Among the various basal media tested, α-minimum essential media and knock out-minimum essential media supplemented with 10%
fetal bovine serum were found to be the most optimal media composition in preserving the phenotypic characteristics and differentiation
potential for prolonged periods as compared with DMEM-F12 and DMEM-LG. Plating density has been shown to affect overall yield.
As a conclusion, the adoption of an appropriate culture system significantly improved cell yield, thus enabling the attainment
of sufficient yields for therapeutic applications economizing in terms of cost of production and minimizing seeding cell density
for maximum yield. 相似文献
118.
Jang IH Chosa N Kim SH Nam HJ Lemaitre B Ochiai M Kambris Z Brun S Hashimoto C Ashida M Brey PT Lee WJ 《Developmental cell》2006,10(1):45-55
The Toll receptor was originally identified as an indispensable molecule for Drosophila embryonic development and subsequently as an essential component of innate immunity from insects to humans. Although in Drosophila the Easter protease processes the pro-Sp?tzle protein to generate the Toll ligand during development, the identification of the protease responsible for pro-Sp?tzle processing during the immune response has remained elusive for a decade. Here, we report a protease, called Sp?tzle-processing enzyme (SPE), required for Toll-dependent antimicrobial response. Flies with reduced SPE expression show no noticeable pro-Sp?tzle processing and become highly susceptible to microbial infection. Furthermore, activated SPE can rescue ventral and lateral development in embryos lacking Easter, showing the functional homology between SPE and Easter. These results imply that a single ligand/receptor-mediated signaling event can be utilized for different biological processes, such as immunity and development, by recruiting similar ligand-processing proteases with distinct activation modes. 相似文献
119.
Kambris Z Brun S Jang IH Nam HJ Romeo Y Takahashi K Lee WJ Ueda R Lemaitre B 《Current biology : CB》2006,16(8):808-813
Unlike mammalian Toll-like Receptors, the Drosophila Toll receptor does not interact directly with microbial determinants but is rather activated upon binding a cleaved form of the cytokine-like molecule Spatzle (Spz). During the immune response, Spz is thought to be processed by secreted serine proteases (SPs) present in the hemolymph that are activated by the recognition of gram-positive bacteria or fungi . In the present study, we have used an in vivo RNAi strategy to inactivate 75 distinct Drosophila SP genes. We then screened this collection for SPs regulating the activation of the Toll pathway by gram-positive bacteria. Here, we report the identification of five novel SPs that function in an extracellular pathway linking the recognition proteins GNBP1 and PGRP-SA to Spz. Interestingly, four of these genes are also required for Toll activation by fungi, while one is specifically associated with signaling in response to gram-positive bacterial infections. These results demonstrate the existence of a common cascade of SPs upstream of Spz, integrating signals sent by various secreted recognition molecules via more specialized SPs. 相似文献
120.
Wolbachia stimulates immune gene expression and inhibits plasmodium development in Anopheles gambiae
Kambris Z Blagborough AM Pinto SB Blagrove MS Godfray HC Sinden RE Sinkins SP 《PLoS pathogens》2010,6(10):e1001143
The over-replicating wMelPop strain of the endosymbiont Wolbachia pipientis has recently been shown to be capable of inducing immune upregulation and inhibition of pathogen transmission in Aedes aegypti mosquitoes. In order to examine whether comparable effects would be seen in the malaria vector Anopheles gambiae, transient somatic infections of wMelPop were created by intrathoracic inoculation. Upregulation of six selected immune genes was observed compared to controls, at least two of which (LRIM1 and TEP1) influence the development of malaria parasites. A stably infected An. gambiae cell line also showed increased expression of malaria-related immune genes. Highly significant reductions in Plasmodium infection intensity were observed in the wMelPop-infected cohort, and using gene knockdown, evidence for the role of TEP1 in this phenotype was obtained. Comparing the levels of upregulation in somatic and stably inherited wMelPop infections in Ae. aegypti revealed that levels of upregulation were lower in the somatic infections than in the stably transinfected line; inhibition of development of Brugia filarial nematodes was nevertheless observed in the somatic wMelPop infected females. Thus we consider that the effects observed in An. gambiae are also likely to be more pronounced if stably inherited wMelPop transinfections can be created, and that somatic infections of Wolbachia provide a useful model for examining effects on pathogen development or dissemination. The data are discussed with respect to the comparative effects on malaria vectorial capacity of life shortening and direct inhibition of Plasmodium development that can be produced by Wolbachia. 相似文献