全文获取类型
收费全文 | 2273篇 |
免费 | 219篇 |
出版年
2023年 | 23篇 |
2022年 | 27篇 |
2021年 | 85篇 |
2020年 | 34篇 |
2019年 | 45篇 |
2018年 | 74篇 |
2017年 | 55篇 |
2016年 | 68篇 |
2015年 | 86篇 |
2014年 | 114篇 |
2013年 | 161篇 |
2012年 | 172篇 |
2011年 | 138篇 |
2010年 | 92篇 |
2009年 | 69篇 |
2008年 | 94篇 |
2007年 | 75篇 |
2006年 | 81篇 |
2005年 | 73篇 |
2004年 | 55篇 |
2003年 | 59篇 |
2002年 | 50篇 |
2001年 | 43篇 |
2000年 | 29篇 |
1999年 | 33篇 |
1998年 | 14篇 |
1997年 | 21篇 |
1996年 | 17篇 |
1995年 | 12篇 |
1994年 | 13篇 |
1993年 | 19篇 |
1992年 | 39篇 |
1991年 | 33篇 |
1990年 | 37篇 |
1989年 | 36篇 |
1988年 | 25篇 |
1987年 | 27篇 |
1986年 | 38篇 |
1985年 | 30篇 |
1984年 | 33篇 |
1983年 | 22篇 |
1982年 | 19篇 |
1981年 | 20篇 |
1980年 | 14篇 |
1979年 | 25篇 |
1978年 | 17篇 |
1977年 | 20篇 |
1976年 | 20篇 |
1975年 | 24篇 |
1974年 | 24篇 |
排序方式: 共有2492条查询结果,搜索用时 15 毫秒
101.
Bhagat Prakash Kumar Verma Deepanjali Sharma Deepika Sinha Alok Krishna 《Plant molecular biology》2021,107(1-2):117-127
Plant Molecular Biology - Cross-talk between light and ABA signaling is mediated by physical interaction between HY5 and ABI5 Arabidopsis. Plants undergo numerous transitions during their... 相似文献
102.
Mahendran Ganesan Kumar Deepak Verma Sanjeet Kumar Chandran Ashish Warsi ·Zafar Iqbal Husain Zakir Afroz Shama Rout Prasant Kumar Rahman Laiq Ur 《Plant Cell, Tissue and Organ Culture》2021,146(2):423-423
Plant Cell, Tissue and Organ Culture (PCTOC) - 相似文献
103.
Choudhury Aparajita Verma Shubham Muthamilarasan Mehanathan Rajam Manchikatla Venkat 《Molecular biology reports》2021,48(11):7477-7485
Molecular Biology Reports - Maruca vitrata is one of the potential insect pests that cause devastating losses to legume cultivation worldwide. Gene functional studies facilitate dissecting the... 相似文献
104.
Yadav Jyoti Verma Anoop Kumar Ahmad Md. Kaleem Garg Ravindra K. Shiuli Mahdi Abbas Ali Srivastava Shrikant 《Molecular biology reports》2021,48(4):3245-3252
Molecular Biology Reports - Alzheimer's disease is a common neurodegenerative disease in the elderly population and a leading cause of dementia. Genetics and environmental risk factors were... 相似文献
105.
Kelsey Bettridge Subhash Verma Xiaoli Weng Sankar Adhya Jie Xiao 《Molecular microbiology》2021,115(1):12-27
HU (Histone‐like protein from Escherichia coli strain U93) is the most conserved nucleoid‐associated protein in eubacteria, but how it impacts global chromosome organization is poorly understood. Using single‐molecule tracking, we demonstrate that HU exhibits nonspecific, weak, and transitory interactions with the chromosomal DNA. These interactions are largely mediated by three conserved, surface‐exposed lysine residues (triK), which were previously shown to be responsible for nonspecific binding to DNA. The loss of these weak, transitory interactions in a HUα(triKA) mutant results in an over‐condensed and mis‐segregated nucleoid. Mutating a conserved proline residue (P63A) in the HUα subunit, deleting the HUβ subunit, or deleting nucleoid‐associated naRNAs, each previously implicated in HU’s high‐affinity binding to kinked or cruciform DNA, leads to less dramatically altered interacting dynamics of HU compared to the HUα(triKA) mutant, but highly expanded nucleoids. Our results suggest HU plays a dual role in maintaining proper nucleoid volume through its differential interactions with chromosomal DNA. On the one hand, HU compacts the nucleoid through specific DNA structure‐binding interactions. On the other hand, it decondenses the nucleoid through many nonspecific, weak, and transitory interactions with the bulk chromosome. Such dynamic interactions may contribute to the viscoelastic properties and fluidity of the bacterial nucleoid to facilitate proper chromosome functions. 相似文献
106.
Paul D. Hartley Richard L. Tillett David P. AuCoin Joel R. Sevinsky Yanji Xu Andrew Gorzalski Mark Pandori Erin Buttery Holly Hansen Michael A. Picker Cyprian C. Rossetto Subhash C. Verma 《遗传学报》2021,48(1):40-51
Patients with signs of COVID-19 were tested through diagnostic RT-PCR for SARS-CoV-2 using RNA extracted from the nasopharyngeal/nasal swabs.To determine the variants of SARS-CoV-2 circulating in the state of Nevada,specimens from 200 COVID-19 patients were sequenced through our robust sequencing platform,which enabled sequencing of SARS-CoV-2 from specimens with even very low viral loads,without the need of culture-based amplification.High genome coverage allowed the identification of single and multi-nucleotide variants in SARS-CoV-2 in the community and their phylogenetic relationships with other variants present during the same period of the outbreak.We report the occurrence of a novel mutation at 323aa (314aa of orf1b) of nsp12 (RNA-dependent RNA polymerase) changed to phenylalanine(F) from proline (P),in the first reported isolate of SARS-CoV-2,Wuhan-Hu-1.This 323F variant was present at a very high frequency in Northern Nevada.Structural modeling determined this mutation in the interface domain,which is important for the association of accessory proteins required for the polymerase.In conclusion,we report the introduction of specific SARS-CoV-2 variants at very high frequency in distinct geographic locations,which is important for understanding the evolution and circulation of SARS-CoV-2variants of public health importance,while it circulates in humans. 相似文献
107.
Amino acid restriction is among promising potential cancer treatment strategies. However, cancer cells employ a multitude of mechanisms to mount resistance to amino acid restriction, which impede the latter’s clinical development. Here we show that MAPK signaling activation in asparagine‐restricted melanoma cells impairs GSK3‐β‐mediated c‐MYC degradation. In turn, elevated c‐MYC supports ATF4 translational induction by enhancing the expression of the amino acid transporter SLC7A5, increasing the uptake of essential amino acids, and the subsequent maintenance of mTORC1 activity in asparagine‐restricted melanoma cells. Blocking the MAPK‐c‐MYC‐SLC7A5 signaling axis cooperates with asparagine restriction to effectively suppress melanoma cell proliferation. This work reveals a previously unknown axis of cancer cell adaptation to asparagine restriction and informs mechanisms that may be targeted for enhanced therapeutic efficacy of asparagine limiting strategies. 相似文献
108.
Chettri Dixita Verma Ashwani Kumar Sarkar Lija Verma Anil Kumar 《Extremophiles : life under extreme conditions》2021,25(3):203-219
Extremophiles - Technological advances in the field of life sciences have led to discovery of organisms that live in harsh environmental conditions referred to as extremophiles. These organisms... 相似文献
109.
AbstractCalcium is an important macronutrient for both prokaryotes and eukaryotes. It acts as an important second messenger mediating rapid response to environmental conditions. The present investigation deals with proteome profiling of Anabaena 7120 and its derivative ntcA mutant in response to varied calcium doses (0, 1 and 10?mM CaCl2). Concentration of 1?mM CaCl2 salt was the optimum concentration whereas 10?mM CaCl2 was the inhibitory concentration for both the wild type and mutant strains. The results showed highly significant alteration in terms of protein abundance and differential response related to key processes of photosynthesis, energy and metabolism, nitrogen metabolism, oxidative and antioxidative defence, transport and signalling and fatty acid metabolism. In the wild type proteins related to photosynthesis and nitrogen metabolism showed upregulation at 1?mM CaCl2 concentration while antioxidative defence related proteins were down-regulated. In the mutant however, proteins related to photosynthesis and nitrogen metabolism exhibited severe down-regulation. Some hypothetical proteins were also realized during proteome analysis. Overall, our results suggested that NtcA have a potential role in regulation of calcium ion dependent key processes underlying in various metabolic activities of the cyanobacterium Anabaena 7120. 相似文献
110.