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11.
Amelioration of municipal sludge by Pistia stratiotes L.: role of antioxidant enzymes in detoxification of metals 总被引:1,自引:0,他引:1
Pistia stratiotes when exposed to mixture of municipal sludge and effluent accumulated substantial amount of metals in the fronds and roots. With the increase in the metal accumulation by the plants, a reduction in the concentration of metals was found in leachates. The treated plants showed reduced level in chlorophylls but enhanced level of carotenoids and protein. The plant showed a concomitant increase in the activities of antioxidant enzymes; superoxide dismutase, guiacol peroxidase and also an enhanced level of lipid peroxidation. The activities were more in the root tissues as compared to frond. Initially a reduced level of cysteine content in roots of sludge treated plant as compared to control was found, but with time duration it increased progressively. The level of non-protein thiols also increased gradually at all the durations in both fronds and roots. Thus, beside the reduction of metals from municipal sludge, the ability of P. stratiotes to combat metal generated damages by induced synthesis of antioxidant enzymes and other metal binding ligands shows its suitability for the phytoremediation of the waste. 相似文献
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Khobragade CN Beedkar SD Bodade RG Vinchurkar AS 《International journal of biological macromolecules》2011,48(3):466-473
In humans oxalate is end product of protein metabolism, with no enzyme present to act on it. In conditions of its enhanced endogenous synthesis or increased absorption from the diet, oxalate accumulation leads to hyperoxaluria which can further lead to a number of pathological conditions including urolithiasis. Urolithiasis has been a perplexing problem due to its high incidence and rate of recurrence after treatment like Extracorporeal-shock wave lithotripsy (ESWL). Hence other prophylactic treatment becomes necessary. One of the newer approaches of curing such metabolic disorders is the enzyme supplementation therapy. Oxalate oxidase (OxOx) is a commonly occurring enzyme in plants, bacteria and fungi that catalyses oxidative cleavage of oxalate to CO(2) with reduction of dioxygen to H(2)O(2). Present study, used Hordeum vulgare OxOx crystal structure (PDB ID 2ET1A) as a template for constructing 3D models of OxOx from Triticum aestivum, Arabidopsis thaliana, Sclerotiana sclerotiarum. Similarly Homology models for isoforms Ceriporiopsis subvermispora 336, C. subvermispora 422 were constructed by using template Bacillus subtilis oxalate decarboxylase (Oxdc) (PDB ID 2UY8A) by comparative modeling approach in SWISS MODEL, MODELLER, 3D JIGSAW and GENO 3D program server. Based on overall stereochemical quality (PROCHECK, PROSA, VARIFY 3D), best models were selected, energy minimized, refined and characterized for active site in BioMed CaChe V 6.1 workspace. Selected models were further studied for structure function relationship with substrate (oxalate) and its analogue (glycolate) by using docking approach. Calculated interaction energy between the oxalate and constructed enzyme indicated that homology models for OxOx of T. aestivum, A. thaliana and S. sclerotiarum, can account for better regio-specificity of this enzyme towards oxalate. That supports the interested metabolism and thus may further implement in enzyme supplementation therapy for urolithiasis. 相似文献
14.
Babasaheb P. Bandgar Shrikant S. Gawande Ragini G. Bodade Nalini M. Gawande Chandrahasya N. Khobragade 《Bioorganic & medicinal chemistry》2009,17(24):8168-8173
A novel series of 1-(2,4-dimethoxy-phenyl)-3-(1,3-diphenyl-1H-pyrazol-4-yl)-propenone (3) have been prepared by the Claisen–Schmidt condensation of 1-(2,4-dimethoxy-phenyl)-ethanone (1) and substituted 1,3-diphenyl-1H-pyrazole-4-carbaldehydes (2). Substituted 1,3-diphenyl-1H-pyrazole-4-carbaldehydes (2) were prepared by Vilsmeir–Haack reaction on acetophenonephenylhydrazones to offer the target compounds. The structures of the compounds were established by IR, 1H NMR and mass spectral analysis. All the compounds were evaluated for their anti-inflammatory (TNF-α and IL-6 inhibitory assays), antioxidant (DPPH free radical scavenging assay) and antimicrobial activities (agar diffusion method) against some pathogenic bacteria and fungi. Of 10 compounds screened, compounds 3a, 3c and 3g exhibited promising IL-6 inhibitory (35–70% inhibition, 10 μM), free radical scavenging (25–35% DPPH activity) and antimicrobial activities (MIC 100 μg/mL and 250 μg/mL) at varied concentrations. The structure–activity relationship (SAR) and in silico drug relevant properties (HBD, HBA, PSA, c Log P, molecular weight, EHOMO and ELUMO) further confirmed that the compounds are potential lead compounds for future drug discovery study. Toxicity of the compounds was evaluated theoretically and experimentally and revealed to be nontoxic except 3d and 3j. 相似文献
15.
Characterization of structures in biofilms formed by a Pseudomonas fluorescens isolated from soil 总被引:1,自引:0,他引:1
Marc M Baum Aleksandra Kainović Teresa O'Keeffe Ragini Pandita Kent McDonald Siva Wu Paul Webster 《BMC microbiology》2009,9(1):103-13
Background
Microbial biofilms represent an incompletely understood, but fundamental mode of bacterial growth. These sessile communities typically consist of stratified, morphologically-distinct layers of extracellular material, where numerous metabolic processes occur simultaneously in close proximity. Limited reports on environmental isolates have revealed highly ordered, three-dimensional organization of the extracellular matrix, which may hold important implications for biofilm physiology in vivo. 相似文献16.
The elaborate networks and the crosstalk of established signaling molecules like salicylic acid (SA), jasmonic acid (JA),
ethylene (ET), abscisic acid (ABA), reactive oxygen species (ROS) and glutathione (GSH) play key role in plant defense response.
To obtain further insight into the mechanism through which GSH is involved in this crosstalk to mitigate biotic stress, transgenic
Nicotiana tabacum overexpressing Lycopersicon esculentum gamma-glutamylcysteine synthetase (LeECS) gene (NtGB lines) were generated with enhanced level of GSH in comparison with wild-type plants exhibiting resistance to pathogenesis
as well. The expression levels of non-expressor of pathogenesis-related genes 1 (NPR1)-dependent genes like pathogenesis-related
gene 1 (NtPR1), mitogen-activated protein kinase kinase (NtMAPKK), glutamine synthetase (NtGLS) were significantly enhanced alongwith NtNPR1. However, the expression levels of NPR1-independent genes like NtPR2, NtPR5 and short-chain dehydrogenase/reductase family protein (NtSDRLP) were either insignificant or were downregulated. Additionally, increase in expression of thioredoxin (NtTRXh), S-nitrosoglutathione reductase 1 (NtGSNOR1) and suppression of isochorismate synthase 1 (NtICS1) was noted. Comprehensive analysis of GSH-fed tobacco BY2 cell line in a time-dependent manner reciprocated the in planta results. Better tolerance of NtGB lines against biotrophic Pseudomonas syringae pv. tabaci was noted as compared to necrotrophic Alternaria alternata. Through two-dimensional gel electrophoresis (2-DE) and image analysis, 48 differentially expressed spots were identified
and through identification as well as functional categorization, ten proteins were found to be SA-related. Collectively, our
results suggest GSH to be a member in cross-communication with other signaling molecules in mitigating biotic stress likely
through NPR1-dependent SA-mediated pathway. 相似文献
17.
Triguna N. Misra Ram S. Singh Janardan Upadhyay Ragini Srivastava 《Phytochemistry》1984,23(2):415-417
From the roots of Vernonia cinerea a new natural sterol and a new aliphatic acid characterized as stigmast-5,17(20)-dien-3β-ol and 26-methylheptacosanoic acid, respectively, have been isolated together with stigmasterol and sitosterol. 相似文献
18.
Ragini Awachat Atish A. Wagh Manisha Aher Moneesha Fernandes Vaijayanti A. Kumar 《Bioorganic & medicinal chemistry letters》2018,28(10):1765-1768
Simple 2′-OMe-chemical modification in the loop region of the 15mer G-rich DNA sequence GGTTGGTGTGGTTGG is reported. The G-quadruplex structure of this thrombin-binding aptamer (TBA), is stabilized by single modifications (T?→?2′-OMe-U), depending on the position of the modification. The structural stability also renders significantly increased inhibition of thrombin-induced fibrin polymerization, a process closely associated with blood-clotting. 相似文献
19.
Ragini Bisaria Padma Vasudevan V. S. Bisaria 《Applied microbiology and biotechnology》1990,33(5):607-609
Summary Various spent agro-residues obtained after cultivation of the edible mushroom Pleurotus sajor-caju were used in anaerobic digestors for production of biogas. The changes that take place in the residues during bioconversion were quantified in terms of composition of cellulose, hemicellulose, lignin, carbon and nitrogen. These mycostraws resulted in increased biogas production over the untreated ones, which varied from 21.5% in the case of spent bagasse to 38.8% in the case of spent paddy straw. The increased biogas generation by the spent residues seems to be due to the increased susceptibility to digestion and more favourable C/N ratio of the residues.
Offprint requests to: V. S. Bisaria 相似文献
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Sharma DC Budania R Shah M Jain P Gaur BL 《Indian journal of experimental biology》2004,42(5):504-507
Three silver preparations (Varak or foil, ash or Raupya bhasma and sol or colloidal solution) were fed to three groups of young, male chicks for 10 days. There was significant fall in all the plasma lipid fractions--total lipids, phospholipids, triglycerides and total cholesterol. There was a marked rise in silver content of plasma and whole blood, ranging from 4 to 13 times, suggesting that the observed hypolipidemic action may be due to silver. The administration of the three silver preparations did not cause any retardation in growth, toxic manifestation, side effect or untoward reaction. 相似文献