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81.
Pérez-del-Olmo A Morand S Raga JA Kostadinova A 《International journal for parasitology》2011,41(13-14):1361-1370
Abundance-occupancy and abundance-variance relationships are two of the most general macroecological patterns capturing essential fundamentals of the structuring of species distributions and are widely documented for free-living animal and plant species populations at different spatial scales. However, empirical data for parasites have been gathered using appropriate sampling designs only recently. We performed analyses across species of the variation in infection parameters and patterns of aggregation of the most widespread parasites in the marine sparid fish Boops boops across seven localities of two marine biogeographical regions, the North East Atlantic and the Mediterranean. We used a large dataset of multiple population samples replicated over time for 20 parasite species and carried out assessments both intraspecifically and interspecifically, across taxonomic and ecological groupings. This taxonomically diverse complex of species representing five major metazoan higher taxa with differing transmission ecologies allowed us to assess the effect of taxonomic and ecological determinants on the abundance-occupancy and abundance-variance relationships in the model marine host-parasite system. The results revealed that: (i) a power function, relating spatial variance to mean abundance, represents a suitable model for the spatial distribution of the species; (ii) prevalence, abundance and the degree of spatial heterogeneity are true species characteristics and differ consistently between higher level taxonomic groupings; (iii) infection parameters and abundance-variance relationship are dependent on host specificity and regional distribution patterns of the parasites; and (iv) the observed infection parameters agree well with predictions from the epidemiological negative binomial abundance-occupancy model built on parameters of Taylor's power law both within and across species. 相似文献
82.
Pérez-del Olmo A Fernández M Gibson DI Raga JA Kostadinova A 《Systematic parasitology》2007,66(2):137-157
Six species of digeneans, including three new host records, are described from the bogue Boops boops off the Spanish NE Atlantic and the Mediterranean coasts. The species involved are: Robphildollfusium martinezgomezi López-Román, Gijón-Botella, Kim & Vilca-Choque, 1992, Magnibursatus caudofilamentosa (Reimer, 1971) Gibson & K?ie, 1991, Lepocreadium album Stossich, 1890, Steringotrema pagelli (van Beneden, 1871) Odhner, 1911, Tetrochetus coryphaenae Yamaguti, 1934 and Stephanostomum euzeti Bartoli & Bray, 2004 (metacercaria). B. boops is a new host for 11 metazoan parasites (six digeneans, three acanthocephalans, one copepod and one isopod) recovered in this study. These are reported and incorporated into a complete checklist of the metazoan parasites of B. boops throughout its distributional range. It comprises summarised information for 67 species in 260 host-parasite records and includes the name of the parasite species, the locality of the host, and the author and date of the published record. The taxonomy is updated and annotations are made on the validity of the records and synonymies. 相似文献
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Ohiana Revuelta Yolanda M. León Juan A. Balbuena Annette C. Broderick Pablo Feliz Brendan J. Godley Juan A. Raga Jesús Tomás 《Biodiversity and Conservation》2014,23(6):1529-1539
Protected areas are considered essential elements for global biodiversity conservation. They may not necessarily result in an effective conservation of resources in developing countries due to lack of funding for management and enforcement. In addition, poor governance aligned with conflicts of economic interests related to their use can further threaten their integrity and persistence. In the Dominican Republic, the western beaches of the Jaragua National Park (JNP), a protected area which is also part of a UNESCO Biosphere Reserve, have been proposed for development using a mass-tourism model. One of the most charismatic species found in this area is the leatherback turtle (Dermochelys coriacea). In the present study, we assess hatching success, and factors affecting it, to determine the reproductive value across the area for the leatherback turtle. The main factors found driving hatching success at the study beaches are beach sector, incubation duration, date of lay and clutch size. Our results show that clutches in La Cueva (located in the buffer zone of the park) and Bahía de las Águilas (located inside the limits of the park) have an unusually high hatching success (~75 %) for this species, highlighting the importance of increasing protection efforts at these sites. We strongly recommend including La Cueva inside the limits of the JNP. 相似文献
88.
High‐throughput ion exchange purification of positively charged recombinant protein in the presence of negatively charged dextran sulfate
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Lam Raga A. Markely Lutfiye Kurt Janet Lau Sarthak Mane Bing Guan Thomas Ryll Scott Estes Shashi Prajapati Meisam Bakhshayeshi John Pieracci 《Biotechnology progress》2014,30(2):516-520
Product quality analyses are critical for developing cell line and bioprocess producing therapeutic proteins with desired critical product quality attributes. To facilitate these analyses, a high‐throughput small‐scale protein purification (SSP) is required to quickly purify many samples in parallel. Here we develop an SSP using ion exchange resins to purify a positively charged recombinant growth factor P1 in the presence of negatively charged dextran sulfate supplemented to improve the cell culture performance. The major challenge in this work is that the strong ionic interaction between P1 and dextran sulfate disrupts interaction between P1 and chromatography resins. To solve this problem, we develop a two‐step SSP using Q Sepharose Fast Flow (QFF) and SP Sepharose XL (SPXL) resins to purify P1. The overall yield of this two‐step SSP is 78%. Moreover, the SSP does not affect the critical product quality attributes. The SSP was critical for developing the cell line and process producing P1. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:516–520, 2014 相似文献
89.
Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
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María López‐Malo Estefani García‐Rios Rosana Chiva José Manuel Guillamon María Martí‐Raga 《Biotechnology progress》2014,30(4):776-783
Low‐temperature fermentations produce wines with greater aromatic complexity, but the success of these fermentations greatly depends on the adaptation of yeast cells to cold. Tryptophan has been previously reported to be a limiting amino acid during Saccharomyces cerevisiae growth at low temperature. The objective of this study was to determine the influence of the tryptophan metabolism on growth and fermentation performance during low‐temperature wine fermentation. To this end, we constructed the deletion mutants of the TRP1 and TAT2 genes in a derivative haploid of a commercial wine strain, and the TAT2 gene was overexpressed in the prototroph and auxotroph (Δtrp1) backgrounds. Then we characterized growth and fermentation activity during wine fermentation at low and optimum temperatures. Our results partially support the role of this amino acid in cold yeast growth. Although deletion of TRP1 impaired amino acid uptake and the growth rate at low temperature in synthetic must, this growth impairment did not affect the fermentation rate. Deletion of TAT2 endorsed this strain with the highest nitrogen consumption capacity and the greatest fermentation activity at low temperature. Our results also evidenced reduced ammonium consumption in all the strains at low temperature. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:776–783, 2014 相似文献
90.
Y Deng J Zhao D Sakurai KM Kaufman JC Edberg RP Kimberly DL Kamen GS Gilkeson CO Jacob RH Scofield CD Langefeld JA Kelly ME Alarcón-Riquelme BIOLUPUS GENLES Networks JB Harley TJ Vyse BI Freedman PM Gaffney KM Sivils JA James TB Niewold RM Cantor W Chen BH Hahn EE Brown PROFILE BP Tsao 《Arthritis research & therapy》2012,14(Z3):A5