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81.
Heavy metal sorption by released polysaccharides and whole cultures of two exopolysaccharide-producing cyanobacteria 总被引:1,自引:0,他引:1
The metal removal capacity of cultures of two capsulated, exopolysaccharide-producing cyanobacteria, Cyanospira capsulata and Nostoc PCC7936, were tested using copper (II) as the model metal. C. capsulata cultures removed the greatest amount of copper, with a maximum per unit of biomass (q
max) of 115.0±5.1 mg copper g−1 of protein, compared with 85.0±3.2 removed with Nostoc PCC7936 cultures. Water solutions of pure polysaccharides (RPSs) released into the culture medium by C. capsulata and Nostoc PCC7936 achieved q
max values of 20.2±0.8 mg g−1 copper per polysaccharide dry weight with C. capsulata RPS and 11.0±1.5 mg g−1 with Nostoc PCC7936 RPS. Cultures of the two cyanobacteria also removed Zn (II) and Ni (II), in both single-metal systems and in multimetal
systems with Cu; in the various single-metal systems more copper was removed than Zn or Ni, while in the multimetal systems
a smaller amount of each individual metal was removed but the overall amount of all metal ions sorbed or the amount of copper
sorbed in the copper-only system was almost the same with C. capsulata, and slightly higher with Nostoc PCC7936. 相似文献
82.
Raffaella Di Lisi Anne Picard Simonetta Ausoni Stefano Schiaffino 《BMC molecular biology》2007,8(1):78
Background
We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter GATA elements in skeletal muscle cells. 相似文献83.
Caretti A Morel S Milano G Fantacci M Bianciardi P Ronchi R Vassalli G von Segesser LK Samaja M 《Experimental biology and medicine (Maywood, N.J.)》2007,232(7):887-894
To study the in vivo dynamics of hypoxia-inducible factor 1alpha (HIF-1alpha), master regulator of O(2)-dependent gene expression, and mitogen-activated protein kinases (MAPKs) in the hypoxic myocardium, Sprague-Dawley rats (n = 4 to 6 per group) were exposed to 1-hr hypoxia (10% O(2)), 23-hr hypoxia, and 23-hr hypoxia, followed by reoxygenation. HIF-1alpha increased 15-fold after 1-hr hypoxia, remained constant for 23 hrs, and returned to baseline on reoxygenation. Extracellular signal-regulated kinases (ERK1/2) were unchanged throughout. Phosphorylated p38 increased 4-fold after 1-hr hypoxia and returned to baseline within 23-hr hypoxia. The activity of stress-activated protein kinases/c-Jun NH(2)-terminal kinases (JNKs), measured as phosphorylated c-Jun, increased 3-fold after 1-hr hypoxia and remained sustained afterward. Furthermore, HIF-1alpha was halved in rats that were administered with the p38 inhibitor SB202190 and made hypoxic for 1 hr. In conclusion, although very sensitive to the reoxygenation, HIF-1alpha is overexpressed in vivo in the hypoxic myocardium, and its acute induction by hypoxia is correlated with that of p38. 相似文献
84.
85.
86.
Donati M Di Francesco A Delucca F Di Paolo M Battilani M Balboni A Baldelli R Cevenini R 《FEMS immunology and medical microbiology》2011,61(1):125-128
It is known that neutralizing species-specific or serovar-specific antibodies are produced in response to chlamydial infection in humans and in some animal species. In a previous study, a strong in vitro neutralizing activity to Chlamydia suis in 80% of sera from C. suis-infected pigs had been observed. In view of the close relationship between C. suis and Chlamydia trachomatis, in the present study, the neutralizing activity against D-K C. trachomatis and C. suis purified elementary bodies (EBs) in sera collected from C. trachomatis-infected patients and C. suis-infected pigs was evaluated. A neutralizing activity of 50-70% was observed in the human sera against the homologous serovar and one to five heterologous C. trachomatis serovars. These sera were also able to neutralize C. suis EBs. The pig sera showed a strong neutralizing activity (70-100%) against C. suis EBs and all eight urogenital C. trachomatis serovars. These results suggested the presence of common immunogenic antigens in C. trachomatis and C. suis. Immunoblot analysis, performed to elucidate the target of this neutralizing activity, showed a clear reactivity in human and pig sera against two proteins of 150 and 40 kDa MW, when tested either with C. trachomatis or with C. suis EBs. 相似文献
87.
In vitro micropropagation by direct organogenesis and somatic embryogenesis via callus was developed for Crambe tataria (Brassicaceae). C. tataria is an endemic species of the Pontic-Pannonic region, but it is also present in Italy, where it is localized in Friuli on
a characteristic grassland formation, called “magredi”. C. tataria is regarded as an endangered species. Leaf and root explants were subjected to plant regulator treatments, which invoked
different morphogenic responses. Leaf explants produced more callus than root explants and a higher amount of callus was obtained
with 1 mg l−1 2,4-D in combination with 2 mg l−1 Kin. Somatic embryogenesis was obtained in calli maintained in a delayed subculture regime on media containing BAP in combination
with NAA. Root explants cultured with BAP combined with NAA developed adventitious rosette shoots. Shoots rooted on half-strength
MS media, and the number of roots per plantlet and their length were heavily dependent on sucrose content. The in vitro regenerated
plantlets were acclimatized ex vitro and a mean of 50% of the plantlets survived and showed a true-to-type growth habit. This
study describes the development of two in vitro micropropagation protocols, via direct organogenesis and via embryogenesis
from callus, that are the basis for the application of in vitro tools for the establishment of basal collections with representative
genetic diversity and for the long-term storage of plant genetic material. 相似文献
88.
89.
Proteomics of the bacterial cross-talk by quorum sensing 总被引:1,自引:0,他引:1
90.
Annamaria Leccese Raffaella Viti Susanna Bartolini 《Central European Journal of Biology》2011,6(2):199-204
Two solvent extraction procedures were used to investigate the extraction efficiency in terms of total antioxidant capacity
and total phenols in apricot fruit. Samples were either sequentially extracted with aqueous ethanol (ethanol/water 80% v/v)
and tetrahydrofuran or directly extracted with tetrahydrofuran. Each extract was analyzed for total antioxidant capacity by
the Trolox Equivalent Antioxidant Capacity (TEAC) assay and total phenols by the Folin-Ciocalteu assay. The results showed
that using sequential solvent extraction, the majority (85%) of the total antioxidant capacity and total phenols was due to
hydrophilic compounds. In tetrahydrofuran direct extractions, the total antioxidant capacity and total phenols were higher
than values obtained with aqueous ethanol and the sum of results obtained from sequential extracts for either total antioxidant
capacity or total phenols was similar to the tetrahydrofuran-extract antioxidant values. A linear correlation between total
antioxidant capacity and total phenols was found and was independent of the solvent extraction method. In conclusion, the
choice of solvent is related to the antioxidant potential of fruit and depends on the food hydrophilic/lipophilic composition. 相似文献