全文获取类型
收费全文 | 5614篇 |
免费 | 420篇 |
国内免费 | 1篇 |
出版年
2023年 | 53篇 |
2022年 | 87篇 |
2021年 | 163篇 |
2020年 | 111篇 |
2019年 | 132篇 |
2018年 | 177篇 |
2017年 | 162篇 |
2016年 | 260篇 |
2015年 | 325篇 |
2014年 | 352篇 |
2013年 | 397篇 |
2012年 | 534篇 |
2011年 | 446篇 |
2010年 | 250篇 |
2009年 | 242篇 |
2008年 | 316篇 |
2007年 | 256篇 |
2006年 | 259篇 |
2005年 | 250篇 |
2004年 | 249篇 |
2003年 | 214篇 |
2002年 | 189篇 |
2001年 | 50篇 |
2000年 | 36篇 |
1999年 | 51篇 |
1998年 | 58篇 |
1997年 | 43篇 |
1996年 | 32篇 |
1995年 | 33篇 |
1994年 | 32篇 |
1993年 | 28篇 |
1992年 | 24篇 |
1991年 | 25篇 |
1990年 | 17篇 |
1989年 | 13篇 |
1988年 | 12篇 |
1987年 | 9篇 |
1986年 | 13篇 |
1985年 | 22篇 |
1984年 | 6篇 |
1983年 | 13篇 |
1982年 | 15篇 |
1981年 | 11篇 |
1978年 | 9篇 |
1974年 | 5篇 |
1973年 | 5篇 |
1972年 | 5篇 |
1969年 | 4篇 |
1968年 | 6篇 |
1961年 | 4篇 |
排序方式: 共有6035条查询结果,搜索用时 15 毫秒
91.
Seminotti Bianca Roginski Ana Cristina Zanatta Ângela Amaral Alexandre Umpierrez Fernandes Thabata Spannenberger Kaleb Pinto da Silva Lucas Henrique Rodrigues Ribeiro Rafael Teixeira Leipnitz Guilhian Wajner Moacir 《Journal of bioenergetics and biomembranes》2021,53(5):525-539
Journal of Bioenergetics and Biomembranes - S-adenosylmethionine (AdoMet) predominantly accumulates in tissues and biological fluids of patients affected by liver dysmethylating diseases,... 相似文献
92.
Martínez-Giménez Juan A. Tabares-Seisdedos Rafael 《Origins of life and evolution of the biosphere》2021,51(2):167-183
Origins of Life and Evolution of Biospheres - The origin of genetic systems is the central problem in the study of the origin of life for which various explanatory hypotheses have been presented.... 相似文献
93.
94.
Phillip Dettleff Rodrigo Zuloaga Marcia Fuentes Juan M. Estrada Alfredo Molina Juan A. Valdés 《Journal of fish biology》2021,98(5):1475-1480
Red cusk-eel (Genypterus chilensis) is a native species with potential for Chilean aquaculture diversification. However, no information exists on the effects of temperature on oxidative stress and eggs quality markers in post-ovulatory eggs and ovary of this species. We determine that high and low temperature generate oxidative damage on post-ovulatory eggs, with no effect on ovary. Temperature induces thermal stress markers expression on post-ovulatory eggs, and modulates antioxidant and eggs quality markers on post-ovulatory eggs and ovary, information to consider for quality evaluation in the red cusk-eel management. 相似文献
95.
96.
97.
Nunes Moisés Ubiratã Schmitz Cardoso Olímpio Rafael Soeth Marcelo Silvano Renato Azevedo Matias Fávaro Luís Fernando 《Hydrobiologia》2021,848(4):929-942
Hydrobiologia - Fishers’ ecological knowledge (FEK) has contributed to a better understanding about the reproduction of fishery resources, especially where biological data are scarce or... 相似文献
98.
Rafael Deliz-Aguirre Fakun Cao Fenja H.U. Gerpott Nichanok Auevechanichkul Mariam Chupanova YeVin Mun Elke Ziska Marcus J. Taylor 《The Journal of cell biology》2021,220(7)
A recurring feature of innate immune receptor signaling is the self-assembly of signaling proteins into oligomeric complexes. The Myddosome is an oligomeric complex that is required to transmit inflammatory signals from TLR/IL1Rs and consists of MyD88 and IRAK family kinases. However, the molecular basis for how Myddosome proteins self-assemble and regulate intracellular signaling remains poorly understood. Here, we developed a novel assay to analyze the spatiotemporal dynamics of IL1R and Myddosome signaling in live cells. We found that MyD88 oligomerization is inducible and initially reversible. Moreover, the formation of larger, stable oligomers consisting of more than four MyD88s triggers the sequential recruitment of IRAK4 and IRAK1. Notably, genetic knockout of IRAK4 enhanced MyD88 oligomerization, indicating that IRAK4 controls MyD88 oligomer size and growth. MyD88 oligomer size thus functions as a physical threshold to trigger downstream signaling. These results provide a mechanistic basis for how protein oligomerization might function in cell signaling pathways. 相似文献
99.
Olívia Moraes Ruberti Andressa Silva Sousa Laís Rosa Viana Moiss Felipe Pereira Gomes Alessandra Medeiros Maria Cristina Cintra Gomes Marcondes Luciano de Figueiredo Borges Carlos Cesar Crestani Cristiano Mostarda Telma Ftima da Cunha Moraes Rafael Renatino Canevarolo Maria Andreia Delbin Bruno Rodrigues 《Journal of cellular physiology》2021,236(2):1105-1115
This study aimed to evaluate the impact of aerobic training (AT) on autonomic, cardiometabolic, ubiquitin‐proteasome activity, and inflammatory changes evoked by myocardial infarction (MI) in ovariectomized rats. Female Wistar rats were ovariectomized and divided into four groups: sedentary + sham (SS), sedentary + MI (SI), AT + sham surgery (TS), AT + MI (TI). AT was performed on a treadmill for 8 weeks before MI. Infarcted rats previously subjected to AT presented improved physical capacity, increased interleukin‐10, and decreased pro‐inflammatory cytokines. Metabolomic analysis identified and quantified 62 metabolites, 9 were considered significant by the Vip Score. SS, SI, and TS groups presented distinct metabolic profiles; however, TI could not be distinguished from the SS group. MI dramatically increased levels of dimethylamine, and AT prevented this response. Our findings suggest that AT may be useful in preventing the negative changes in functional, inflammatory, and metabolic parameters related to MI in ovariectomized rats. 相似文献
100.
Mohammed R. Abdullah María T. Batuecas Franziska Jennert Franziska Voß Philipp Westhoff Thomas P. Kohler Rafael Molina Stephanie Hirschmann Michael Lalk Juan A. Hermoso Sven Hammerschmidt 《Journal of molecular biology》2021,433(2):166723
Nucleotides are important for RNA and DNA synthesis and, despite a de novo synthesis by bacteria, uptake systems are crucial. Streptococcus pneumoniae, a facultative human pathogen, produces a surface-exposed nucleoside-binding protein, PnrA, as part of an ABC transporter system. Here we demonstrate the binding affinity of PnrA to nucleosides adenosine, guanosine, cytidine, thymidine and uridine by microscale thermophoresis and indicate the consumption of adenosine and guanosine by 1H NMR spectroscopy. In a series of five crystal structures we revealed the PnrA structure and provide insights into how PnrA can bind purine and pyrimidine ribonucleosides but with preference for purine ribonucleosides. Crystal structures of PnrA:nucleoside complexes unveil a clear pattern of interactions in which both the N- and C- domains of PnrA contribute. The ribose moiety is strongly recognized through a conserved network of H-bond interactions, while plasticity in loop 27–36 is essential to bind purine- or pyrimidine-based nucleosides.Further, we deciphered the role of PnrA in pneumococcal fitness in infection experiments. Phagocytosis experiments did not show a clear difference in phagocytosis between PnrA-deficient and wild-type pneumococci. In the acute pneumonia infection model the deficiency of PnrA attenuated moderately virulence of the mutant, which is indicated by a delay in the development of severe lung infections. Importantly, we confirmed the loss of fitness in co-infections, where the wild-type out-competed the pnrA-mutant. In conclusion, we present the PnrA structure in complex with individual nucleosides and show that the consumption of adenosine and guanosine under infection conditions is required for virulence. 相似文献