全文获取类型
收费全文 | 373篇 |
免费 | 16篇 |
国内免费 | 1篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 10篇 |
2020年 | 14篇 |
2019年 | 9篇 |
2018年 | 10篇 |
2017年 | 6篇 |
2016年 | 11篇 |
2015年 | 16篇 |
2014年 | 13篇 |
2013年 | 30篇 |
2012年 | 24篇 |
2011年 | 32篇 |
2010年 | 21篇 |
2009年 | 25篇 |
2008年 | 29篇 |
2007年 | 28篇 |
2006年 | 20篇 |
2005年 | 14篇 |
2004年 | 19篇 |
2003年 | 14篇 |
2002年 | 14篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1990年 | 3篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 2篇 |
1977年 | 2篇 |
1976年 | 3篇 |
排序方式: 共有390条查询结果,搜索用时 31 毫秒
31.
32.
An expression vector constructed from genes of Pichia pastoris was applied for heterologous gene expression in Saccharomyces cerevisiae. Recombinant hepatitis B surface antigen was synthesized by cloning hepatitis B virus ‘S’ gene under the control of glyceraldehyde-3-phosphate
dehydrogenase (GAP) promoter of Pichia pastoris in Saccharomyces cerevisiae. Hepatitis B surface antigen was constitutively expressed, was stable and exhibited ∼20–22 nm particle formation. Stability
and absence of toxicity to the host with the expression vector indicates the expression system can be applied for large-scale
production. 相似文献
33.
Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNP 总被引:1,自引:1,他引:0 下载免费PDF全文
An enzyme is frequently conceived of as having a single functional mechanism. This is particularly true for motor enzymes, where the necessity for tight coupling of mechanical and chemical cycles imposes rigid constraints on the reaction pathway. In mixtures of substrate (ATP) and an inhibitor (adenosine 5'-(beta,gamma-imido)triphosphate or AMP-PNP), single kinesin molecules move on microtubules in two distinct types of multiple-turnover "runs" that differ in their susceptibility to inhibition. Longer (less susceptible) runs are consistent with movement driven by the alternating-sites mechanism previously proposed for uninhibited kinesin. In contrast, kinesin molecules in shorter runs step with AMP-PNP continuously bound to one of the two active sites of the enzyme. Thus, in this mixture of substrate and inhibitor, kinesin can function as a motor enzyme using either of two distinct mechanisms. In one of these, the enzyme can accomplish high-duty-ratio processive movement without alternating-sites ATP hydrolysis. 相似文献
34.
Prameela J. Ramakrishnaiah H. Krishna V. Deepalakshmi A. P. Naveen Kumar N. Radhika R. N. 《Physiology and Molecular Biology of Plants》2015,21(3):441-446
Physiology and Molecular Biology of Plants - Henckelia incana is an endemic medicinal plant used for the treatment of fever and skin allergy. In the present study shoot regeneration was evaluated... 相似文献
35.
36.
Lovell HC Jackson RW Mansfield JW Godfrey SA Hancock JT Desikan R Arnold DL 《Molecular Plant Pathology》2011,12(2):167-176
The co-evolution of bacterial plant pathogens and their hosts is a complex and dynamic process. Plant resistance can impose stress on invading pathogens that can lead to, and select for, beneficial changes in the bacterial genome. The Pseudomonas syringae pv. phaseolicola (Pph) genomic island PPHGI-1 carries an effector gene, avrPphB (hopAR1), which triggers the hypersensitive reaction in bean plants carrying the R3 resistance gene. Interaction between avrPphB and R3 generates an antimicrobial environment within the plant, resulting in the excision of PPHGI-1 and its loss from the genome. The loss of PPHGI-1 leads to the generation of a Pph strain able to cause disease in the plant. In this study, we observed that lower bacterial densities inoculated into resistant bean (Phaseolus vulgaris) plants resulted in quicker PPHGI-1 loss from the population, and that loss of the island was strongly influenced by the type of plant resistance encountered by the bacteria. In addition, we found that a number of changes occurred in the bacterial genome during growth in the plant, whether or not PPHGI-1 was lost. We also present evidence that the circular PPHGI-1 episome is able to replicate autonomously when excised from the genome. These results shed more light onto the plasticity of the bacterial genome as it is influenced by in planta conditions. 相似文献
37.
Gowda SJ Radhika P Mhase LB Jamadagni BM Gupta VS Kadoo NY 《Journal of applied genetics》2011,52(1):9-21
Chickpea is one of the most important leguminous cool season food crops, cultivated prevalently in South Asia and Middle East.
The main objective of this study was to identify quantitative trait loci (QTLs) associated with seven agronomic and yield
traits in two recombinant inbred line populations of chickpea derived from the crosses JG62 × Vijay (JV population) and Vijay × ICC4958
(VI population) from at least three environments. Single locus QTL analysis involved composite interval mapping (CIM) for
individual traits and multiple-trait composite interval mapping (MCIM) for correlated traits to detect pleiotropic QTLs. Two-locus
analysis was conducted to identify the main effect QTLs (M-QTLs), epistatic QTLs (E-QTLs) and QTL × environment interactions.
Through CIM analysis, a total of 106 significant QTLs (41 in JV and 65 in VI populations) were identified for the seven traits,
of which one QTL each for plant height and days to maturity was common in both the populations. Six pleiotropic QTLs that
were consistent over the environments were also identified. LG2 in JV and LG1a in VI contained at least one QTL for each trait.
Hence, concentrating on these LGs in molecular breeding programs is most likely to bring simultaneous improvement in these
traits. 相似文献
38.
39.
40.