全文获取类型
收费全文 | 488篇 |
免费 | 23篇 |
出版年
2023年 | 2篇 |
2021年 | 12篇 |
2020年 | 6篇 |
2019年 | 17篇 |
2018年 | 16篇 |
2017年 | 15篇 |
2016年 | 13篇 |
2015年 | 23篇 |
2014年 | 28篇 |
2013年 | 38篇 |
2012年 | 33篇 |
2011年 | 34篇 |
2010年 | 26篇 |
2009年 | 24篇 |
2008年 | 36篇 |
2007年 | 30篇 |
2006年 | 31篇 |
2005年 | 19篇 |
2004年 | 20篇 |
2003年 | 8篇 |
2002年 | 11篇 |
2001年 | 9篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 3篇 |
1996年 | 1篇 |
1994年 | 2篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1975年 | 1篇 |
1971年 | 2篇 |
1969年 | 2篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1958年 | 2篇 |
1957年 | 3篇 |
1956年 | 4篇 |
排序方式: 共有511条查询结果,搜索用时 109 毫秒
501.
502.
The efficiency of formation of the ternary complex consisting of the elongation factor Tu and Phe-tRNA’s fromEscherichia coli andStreptomyces aureofaciens was tested to explain the lower activity of thein vitro poly(U) translation system fromS. aureofaciens. Both factors were shown to be functionally interchangeable in the ternary complex formation with Phe-tRNA from eitherE. coli orS. aureofaciens. However, the efficiency of binding ofS. aureofaciens Phe-tRNA to EF-Tu was much lower with both factors. 相似文献
503.
The rates of RNA, protein and DNA synthesis were estimated in synchronously germinating spores ofStreptomyces granaticolor. Rapid uptake of labelled precursors of RNA and proteins was observed after 20 s. The germination process took place through
a sequence of time + ordered events. RNA synthesis started after 3 min of germination, protein synthesis began at 4 min and
net DNA synthesis at 60–70 min of germination. A characteristic feature of germination was the biphasic pattern in the rate
of RNA and protein synthesis. Spores ofStreptomyces granaticolor were sensitive to actinomycin D, rifampicin and chloramphenicol even at the start of germination. Protein synthesis during
germination was dependent on new mRNA synthesis and was independent during the first 60–70 min on replication of the spore
genome. 相似文献
504.
Jindra Windrichova Pavel Broz Radka Fuchsova Ondrej Topolcan Ladislav Pecen Otto Mayer Radek Kucera 《Journal of Medical Biochemistry》2021,40(3):277
BackgroundTo compare four automated immunoassays for the measurement of 25(OH)-vitamin D (25-OHD) and to assess the impact on the results obtained from a healthy population.MethodsWe analysed 100 serum samples on Unicel DxI 800 (Beckman Coulter), Architect i1000 (Abbott), Cobas e411 (Roche) and Liaison XL (DiaSorin). Passing-Bablok regression and Bland-Altman plots were used for method comparison. In order to categorise the obtained values, results were categorised into the following groups: 0-25 nmol/L, 25-50 nmol/L, 50-75 nmol/L and above 75 nmol/L and compared. The percentage of samples below 75 nmol/L, and below 50 nmol/L was then calculated for every method.ResultsAccording to paired comparisons, each method differs from others (p<0.0001) except Cobas vs Architect, which do not show a statistically significant difference (p=0.39). The strongest correlation was found between Liaison and Architect (ρ=0.94, p<0.0001). The percentage of samples below the recommended value of 75 nmol/L were: 70% (Architect), 92% (Liaison), 71% (Cobas) and 89% (Unicel). The percentage of samples below the value of 50 nmol/L were: 17% (Architect), 55% (Liaison), 28% (Cobas) and 47% (Unicel).ConclusionsThe observed differences stem from the use of different analytical systems for 25-OHD concentration analysis and can result in different outcomes. The recommended values should be established for each assay in accordance with the data provided by the manufacturer or in the laboratory, in accordance with proper standardisation. 相似文献
505.
Molecular and functional properties of protein SS1 from small ribosomal subunits of Streptomyces aureofaciens 总被引:1,自引:0,他引:1
Small ribosomal subunits of gram-positive cells of Streptomyces aureofaciens contain an acidic protein designated SS1. Purified protein SS1 has the same mobility in sodium dodecyl sulfate/polyacrylamide gel as ribosomal protein S1 of Escherichia coli (apparent Mr 68 000). Protein SS1 was dissected under mild conditions with trypsin and generated fragments were compared with well-characterized fragments of protein S1. The protein SS1 contains a structure homologous with the C-terminal fragment of protein S1. The affinity of protein SS1 to poly(U) is virtually identical with that of E. coli protein S1. In contrast to protein S1, the addition of SS1 to partially S1-depleted ribosomes of E. coli had no stimulatory effect on poly(U)-directed synthesis of polyphenylalanine. At molar excess of SS1 over ribosomes, the protein had comparable inhibitory effect on polypeptide synthesis as had S1 of E. coli. Ribosomes of S. aureofaciens required about one order of magnitude higher concentration of poly(U) for maximum synthetic activity than did ribosomes of E. coli. The addition of proteins SS1 or S1 to ribosomes of S. aureofaciens had no stimulatory effect on translation of poly(U). Our data indicate that the high-molecular-mass acidic protein SS1 of small ribosomal subunits of S. aureofaciens exhibits only a part of the functional properties of E. coli protein S1. 相似文献
506.
507.
Radek Vodrážka 《Ichnos》2013,20(3):199-201
The validity of the little-known ichnospecies Entobia exogyrarum (Fri?) is verified. Shells of an oyster, Rhynchostreon suborbiculatum (Lam.), represent the substrate for the sponge borings. The borings occur in many Upper Cenomanian to Middle Turonian localities of the Bohemian Cretaceous Basin. This study revealed that the sponges attacked shells of living oysters. Entobia exogyrarum (Fri?) also represents one of the shallowest occurrences of Entobia borings in the Upper Cretaceous rocks. 相似文献
508.
Adam Miszta Radek Machán Ales Benda Andre J Ouellette Wim Th Hermens Martin Hof 《Journal of peptide science》2008,14(4):503-509
The present study has two main objectives. The first is to characterize antimicrobial peptide (AMP) cryptdin-4 (Crp-4) interactions with biological membranes and to compare those interactions with those of magainin 2. The second is to combine the complementary experimental approaches of laser scanning microscopy (LSM), ellipsometry, and Z-scan fluorescence correlation spectroscopy (FCS) to acquire comprehensive information on mechanisms of AMP interactions with supported phospholipid bilayers (SPBs)-a popular model of biological membranes. LSM shows appearance of inhomogeneities in spatial distribution of lipids in the bilayer after treatment with Crp-4. Ellipsometric measurements show that binding of Crp-4 does not significantly change the lipid structure of the bilayer (increase in adsorbed mass without a change in thickness of adsorbed layer). Furthermore, Crp-4 slows the lateral diffusion of lipids within the membrane as shown by Z-scan FCS. All changes of the bilayer induced by Crp-4 can be partially reversed by flushing the sample with excess of buffer. Bilayer interactions of magainin 2 are significantly different, causing large loss of lipids and extensive damage to the bilayer. It seems likely that differences in peptide mode of action, readily distinguished using these combined experimental methods, are related to the distinctive beta-sheet and alpha-helical structures of the respective peptides. 相似文献
509.
Elika Kuthanov Trskov David Bína Stefano Santabarbara Roman Sobotka Radek Kaa Erica Belgio 《Physiologia plantarum》2019,166(1):309-319
In the present paper, we report an improved method combining sucrose density gradient with ion‐exchange chromatography for the isolation of pure chlorophyll a/c antenna proteins from the model cryptophytic alga Rhodomonas salina. Antennas were used for in vitro quenching experiments in the absence of xanthophylls, showing that protein aggregation is a plausible mechanism behind non‐photochemical quenching in R. salina. From sucrose gradient, it was also possible to purify a functional photosystem I supercomplex, which was in turn characterized by steady‐state and time‐resolved fluorescence spectroscopy. R. salina photosystem I showed a remarkably fast photochemical trapping rate, similar to what recently reported for other red clade algae such as Chromera velia and Phaeodactylum tricornutum. The method reported therefore may also be suitable for other still partially unexplored algae, such as cryptophytes. 相似文献
510.
The Fossil Record of Predation, edited by Michal Kowalewski and Patricia H. Kelley, 2002, The Paleontological Society Papers, Volume 8, 398 p., US$20.00, ISSN 1089-3326. Reviewed by Paul D. Taylor, Department of Palaeontology, The Natural History Museum, London SW7 5BD, United Kingdom 相似文献