全文获取类型
收费全文 | 6240篇 |
免费 | 561篇 |
国内免费 | 1篇 |
出版年
2024年 | 7篇 |
2023年 | 48篇 |
2022年 | 129篇 |
2021年 | 247篇 |
2020年 | 132篇 |
2019年 | 127篇 |
2018年 | 152篇 |
2017年 | 137篇 |
2016年 | 227篇 |
2015年 | 339篇 |
2014年 | 389篇 |
2013年 | 462篇 |
2012年 | 577篇 |
2011年 | 609篇 |
2010年 | 327篇 |
2009年 | 289篇 |
2008年 | 339篇 |
2007年 | 349篇 |
2006年 | 329篇 |
2005年 | 282篇 |
2004年 | 246篇 |
2003年 | 245篇 |
2002年 | 248篇 |
2001年 | 44篇 |
2000年 | 37篇 |
1999年 | 62篇 |
1998年 | 51篇 |
1997年 | 43篇 |
1996年 | 34篇 |
1995年 | 33篇 |
1994年 | 25篇 |
1993年 | 18篇 |
1992年 | 21篇 |
1991年 | 15篇 |
1990年 | 7篇 |
1989年 | 9篇 |
1988年 | 13篇 |
1987年 | 6篇 |
1985年 | 7篇 |
1984年 | 13篇 |
1983年 | 12篇 |
1982年 | 6篇 |
1981年 | 9篇 |
1980年 | 13篇 |
1978年 | 5篇 |
1977年 | 7篇 |
1976年 | 9篇 |
1975年 | 7篇 |
1961年 | 5篇 |
1960年 | 5篇 |
排序方式: 共有6802条查询结果,搜索用时 765 毫秒
991.
Manuel Jara Alba Frias-De-Diego Roberto García-Roa Mónica Saldarriaga-Córdoba Lilly P. Harvey Rachel P. Hickcox Daniel Pincheira-Donoso 《Evolutionary biology》2018,45(3):259-267
Chemical communication plays a pivotal role in shaping sexual and ecological interactions among animals. In lizards, fundamental mechanisms of sexual selection such as female mate choice have rarely been shown to be influenced by quantitative phenotypic traits (e.g., ornaments), while chemical signals have been found to potentially influence multiple forms of sexual and social interactions, including mate choice and territoriality. Chemical signals in lizards are secreted by glands primarily located on the edge of the cloacae (precloacal glands, PG) and thighs (femoral glands), and whose interspecific and interclade number ranges from 0 to >?100. However, elucidating the factors underlying the evolution of such remarkable variation remains an elusive endeavour. Competing hypotheses suggest a dominant role for phylogenetic conservatism (i.e., species within clades share similar numbers of glands) or for natural selection (i.e., their adaptive diversification results in deviating numbers of glands from ancestors). Using the prolific Liolaemus lizard radiation from South America (where PG vary from 0 to 14), we present one of the largest-scale tests of both hypotheses to date. Based on climatic and phylogenetic modelling, we show a clear role for both phylogenetic inertia and adaptation underlying gland variation: (i) solar radiation, net primary productivity, topographic heterogeneity and precipitation range have a significant effect on PG variation, (ii) humid and cold environments tend to concentrate species with a higher number of glands, (iii) there is a strong phylogenetic signal that tends to conserve the number of PG within clades. Collectively, our study confirms that the inertia of niche conservatism can be broken down by the need of species facing different selection regimes to adjust their glands to suit the demands of their specific environments. 相似文献
992.
Laminaria is an abundant kelp genus in temperate nearshore ecosystems that grows with a circannual ‘stop-start’ pattern. Species of Laminaria play important ecological roles in kelp forests worldwide and are harvested commercially as a source of food and valuable extracts. In order to evaluate seasonal differences in tissue properties and composition, we compared the material properties, histology and cell-wall composition of overwintering blades with newly synthesized, actively growing blades from Laminaria setchellii. We found that overwintering blades were fortified with a thicker cortex and increased cell wall investment, leading to increased material strength. Overwintering tissues were composed of higher proportions of cellulose and fucose-containing polysaccharides (i.e. FCSPs, fucoidans) than newly formed blades and were found to possess thicker cell walls, likely to withstand the waves of winter storms. Chemical cell wall profiling revealed that significant proportions of fucose were associated with cellulose, especially in overwintering tissues, confirming the association between cellulose and some fucose-containing polysaccharides. Changes in material properties during the resting phase may allow these kelps to retain their non-growing blades through several months of winter storms. The results of this study demonstrate how one species might regulate its material properties seasonally, and at the same time shed light on the mechanisms that might control the material properties of kelps in general. 相似文献
993.
Leah K. Forsberg Rachel E. Davis Virangika K. Wimalasena Brian S.J. Blagg 《Bioorganic & medicinal chemistry》2018,26(12):3096-3110
Inhibition of the Hsp90 C-terminus is an attractive therapeutic approach for the treatment of cancer. Novobiocin, the first Hsp90 C-terminal inhibitor identified, contains a synthetically complex noviose sugar that has limited the generation of structure-activity relationships for this region of the molecule. The work described herein utilizes various ring systems as noviose surrogates to explore the size and nature of the surrounding binding pocket. 相似文献
994.
Future vulnerability mapping based on response to extreme climate events: Dieback thresholds in an endemic California oak
下载免费PDF全文
![点击此处可从《Diversity & distributions》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Blair C. McLaughlin Rachel V. Blakey Naia Morueta‐Holme 《Diversity & distributions》2018,24(9):1186-1198
Aim
This study presents a bioclimate modelling approach, using responses to extreme climate events, rather than historical distributional associations, to project future species vulnerability and refugia. We aim to illustrate the compounding effects of groundwater loss and climate on species vulnerability.Location
California, USA.Methods
As a case study, we used the 2012–2015 California drought and resulting extensive dieback of blue oak (Quercus douglasii). We used aerial dieback surveys, downscaled climate data and subsurface water change data to develop boosted regression tree models identifying key thresholds associated with dieback throughout the blue oak distribution. We (1) combined observed dieback–climatic threshold relationships with climate futures to anticipate future areas of vulnerability and (2) used satellite‐derived measurements of subsurface water loss in drought/dieback modelling to capture the mediating effect of groundwater on species response to climatic drought.Results
A model including climate, climate anomalies and subsurface water change explained 46% of the variability in dieback. Precipitation in 2015 and subsurface water change accounted for 62.6% of the modelled probability of dieback. We found an interaction between precipitation and subsurface water in which dieback probability increased with low precipitation and subsurface water loss. The relationship between precipitation and dieback was nonlinear, with 99% of dieback occurring in areas that received <363 mm precipitation. Based on a MIROC_rcp85 future climate scenario, relative to historical conditions, 13% of the blue oak distribution is predicted to experience more frequent years below this precipitation threshold by mid‐century and 81% by end of century.Main conclusions
As ongoing climate change and extreme events impact ecological processes, the identification of thresholds associated with observed dieback may be combined with climate futures to help identify vulnerable populations and refugia and prioritize climate change‐related conservation efforts. 相似文献995.
996.
Alexis Catala Rachel Culp-Hill Travis Nemkov Angelo D’Alessandro 《Metabolomics : Official journal of the Metabolomic Society》2018,14(7):100
Introduction
Mass spectrometry and computational biology have advanced significantly in the past ten years, bringing the field of metabolomics a step closer to personalized medicine applications. Despite these analytical advancements, collection of blood samples for routine clinical analysis is still performed through traditional blood draws.Objective
TAP capillary blood collection has been recently introduced for the rapid, painless draw of small volumes of blood (~?100 μL), though little is known about the comparability of metabolic phenotypes of blood drawn via traditional venipuncture and TAP devices.Methods
UHPLC-MS-targeted metabolomics analyses were performed on blood drawn traditionally or through TAP devices from 5 healthy volunteers. Absolute quantitation of 45 clinically-relevant metabolites was calculated against stable heavy isotope-labeled internal standards.Results
Ranges for 39 out of 45 quantified metabolites overlapped between drawing methods. Pyruvate and succinate were over threefold higher in the TAP samples than in traditional blood draws. No significant changes were observed for other carboxylates, glucose or lactate. TAP samples were characterized by increases in reduced glutathione and decreases in urate and cystine, markers of oxidation of purines and cysteine—overall suggesting decreased oxidation during draws. The absolute levels of bile acids and acyl-carnitines, as well as almost all amino acids, perfectly correlated among groups (Spearman r?≥?0.95).Conclusion
Though further more extensive studies will be mandatory, this pilot suggests that TAP-derived blood may be a logistically-friendly source of blood for large scale metabolomics studies—especially those addressing amino acids, glycemia and lactatemia as well as bile acids, acyl-carnitine levels.997.
Danusa Mar Arcego Ana Paula Toniazzo Rachel Krolow Carine Lampert Carolina Berlitz Emily dos Santos Garcia Fabrício do Couto Nicola Juliana Bender Hoppe Mariana Maier Gaelzer Caroline Peres Klein Camilla Lazzaretti Carla Dalmaz 《Molecular neurobiology》2018,55(4):2740-2753
During development, the brain goes through fundamental processes, including organization of neural networks and plasticity. Environmental interventions may change initial brain programming, leading to long-lasting effects and altering the susceptibility to psychopathologies, including depression disorder. It is known that depression is a psychiatric disorder with a high prevalence worldwide, including high rates among adolescents. In this study, we evaluated whether social isolation in the prepubertal period and chronic use of high-fat diet (HFD) may induce depressive-like behavior in male adult rats. We also investigated hippocampal plasticity markers and neurotransmitter systems. We found both social isolation and HFD induced a depressive-like behavior in the forced swimming task. Moreover, chronic HFD reduced synaptic markers in hippocampus, demonstrated by reductions in βIII-tubulin (neuronal marker), PSD-95, SNAP-25, and neurotrophin-3. The HFD group also presented decreased glutamatergic and GABAergic receptors subunits. On the other hand, stress affected hippocampal brain-derived neurotrophic factor (BDNF) signaling pathways, and increased expression of subunit of the NMDA receptor (NR2A). Both factors (stress and diet) decreased GR in the hippocampus without affecting plasma corticosterone at basal levels. Interactions between early stress and HFD access were observed only in the BNDF receptor (tropomyosin receptor kinase B; TrkB) and synaptophysin. In summary, these findings showed that a brief social isolation and chronic HFD, during a sensitive developmental period, cause depressive-like behavior in adulthood. The mechanisms underlying these behavioral effects may involve changes in the levels of synaptic proteins in hippocampus: HFD consumption appears to affect synaptic markers, while social isolation affected BDNF signaling more significantly. 相似文献
998.
Rachel J. Errington Maria Sadiq Laura Cosentino Marie Wiltshire Omair Sadiq Marcella Sini Enric Lizano Maria D. Pujol Goreti R. Morais Klaus Pors 《Bioorganic & medicinal chemistry letters》2018,28(8):1274-1277
Structural features from the anticancer prodrug nemorubicin (MMDX) and the DNA-binding molecule DRAQ5? were used to prepare anthraquinone-based compounds, which were assessed for their potential to interrogate cytochrome P450 (CYP) functional activity and localisation. 1,4-disubstituted anthraquinone 8 was shown to be 5-fold more potent in EJ138 bladder cancer cells after CYP1A2 bioactivation. In contrast, 1,5-bis((2-morpholinoethyl)amino) substituted anthraquinone 10 was not CYP-bioactivated but was shown to be fluorescent and subsequently photo-activated by a light pulse (at a bandwidth 532–587?nm), resulting in punctuated foci accumulation in the cytoplasm. It also showed low toxicity in human osteosarcoma cells. These combined properties provide an interesting prospective approach for opto-tagging single or a sub-population of cells and seeking their location without the need for continuous monitoring. 相似文献
999.
1000.
Seasonal dynamics of agonistic behavior and hormones in an ex situ all‐male colony of large flying foxes
下载免费PDF全文
![点击此处可从《Zoo biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Hani D. Freeman Michelle Wood Mandi W. Schook Katherine A. Leighty Shana R. Lavin Susan Wiebe Tracy E. Blowers Rachel Daneault Natalie Mylniczenko Catharine J. Wheaton 《Zoo biology》2018,37(4):213-222
Large flying foxes (Pteropus vampyrus) are a socially complex species. In situ colonies typically comprise thousands of individuals in small harems of one male to many females. In ex situ environments, all‐male colonies are becoming more common due to a surplus of males in the population. There is limited information describing the hormonal and behavioral patterns of all‐male colonies during the breeding season. We assessed seasonal changes in hormones and behavior in an all‐male colony of 12 large flying foxes at Disney's Animal Kingdom®. We validated hormone assays using morning urine and fecal samples to assess seasonal changes in excreted immunoreactive testosterone and glucocorticoid metabolites. We collected behavior data using an all‐occurrence method, recording agonistic behaviors related to territorial defense (hooking, biting, wing flexing, vocalizing, and wrestling), and sexual behavior (mounting and frontal grabbing). Results indicated that (i) we could reliably measure testosterone and glucocorticoid metabolites concentrations from fecal and urine samples collected from individual bats; (ii) there were distinct relationships between changes in levels of agonism and hormone concentrations throughout the year; and (iii) three agonistic behaviors (chasing, wrestling, and open‐mouth threat) peaked prior to the increase in testosterone and glucocorticoid hormones measured during the breeding season. These three behaviors could potentially be used as early indicators to signal the onset of the breeding season and allow time to implement ex situ management changes to reduce the incidence of agonism between individuals. 相似文献