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991.
992.
The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes 总被引:16,自引:0,他引:16
Mark G.M. Aarts Rachel Hodge Kriton Kalantidis Dion Florack Zoe A. Wilson Bernard J. Mulligan Willem J. Stiekema Rod Scott y Pereira 《The Plant journal : for cell and molecular biology》1997,12(3):615-623
The Arabidopsis thaliana MALE STERILITY 2 ( MS2 ) gene product is involved in male gametogenesis. The first abnormalities in pollen development of ms2 mutants are seen at the stage in microsporogenesis when microspores are released from tetrads. Expression of the MS2 gene is observed in tapetum of wild-type flowers at, and shortly after, the release of microspores from tetrads. The MS2 promoter controls GUS expression at a comparable stage in the tapetum of transgenic tobacco containing an MS2 promoter–GUS fusion. The occasional pollen grains produced by mutant ms2 plants have very thin pollen walls. They are also sensitive to acetolysis treatment, which is a test for the presence of an exine layer. The MS2 gene product shows sequence similarity to a jojoba protein that converts wax fatty acids to fatty alcohols. A possible function of the MS2 protein as a fatty acyl reductase in the formation of pollen wall substances is discussed. 相似文献
993.
Environmental gradients drive variation in community composition across a range of spatial scales. In alpine regions, areas of long‐lasting snow (‘snow patches’) create snowmelt gradients that drive considerable change in vegetation structure and composition over small spatial scales. This study examined whether there is parallel variation in arthropod communities using snowmelt gradients in the Australian Alps. Mites (Acarina) were the most common arthropods in snow patches, followed by springtails while, among the insects, the orders Hymenoptera (primarily Formicidae), Diptera, Coleoptera (primarily Carabidae) and Hemiptera (primarily Cicadellidae) dominated. Along the snowmelt gradient, arthropod assemblages changed from having equal proportions of predators and herbivores in early‐melting zones to being predator‐dominated in late‐melting zones, particularly early in the growing season. This followed a transition in vegetation cover and composition and was driven by higher numbers of predacious carabid beetles in later‐melting zones. Overall, however, our results suggest that snowbed arthropod communities in the Australian alpine zone are more sensitive to short‐term effects, such as time since snowmelt, than to differences in vegetation structure and composition or long‐term patterns of snowmelt. Continued advancement of snowmelt timing due to warmer spring temperatures is therefore likely to have more impact on the seasonality of snowbed arthropod communities than on the overall community composition. 相似文献
994.
Sura Shayna A. Delgadillo Aaron Franco Nancy Gu Kelly Turba Rachel Fong Peggy 《Coral reefs (Online)》2019,38(3):425-429
Coral Reefs - Closely cropped algal turfs are characteristic of healthy coral reefs, but unchecked growth can cause transitions into long sediment-laden turfs, which may be an alternative degraded... 相似文献
995.
Gingold-Belfer Rachel Levy Sigal Layfer Olga Pakanaev Lea Niv Yaron Dickman Ram Perets Tsachi Tsadok 《Probiotics and antimicrobial proteins》2020,12(1):112-118
Probiotics and Antimicrobial Proteins - Lactose intolerance is a common condition caused by lactase deficiency and may result in symptoms of lactose malabsorption (bloating, flatulence, abdominal... 相似文献
996.
Beblo K Douki T Schmalz G Rachel R Wirth R Huber H Reitz G Rettberg P 《Archives of microbiology》2011,193(11):797-809
In this study, we investigated the ability of several (hyper-) thermophilic Archaea and phylogenetically deep-branching thermophilic
Bacteria to survive high fluences of monochromatic UV-C (254 nm) and high doses of ionizing radiation, respectively. Nine
out of fourteen tested microorganisms showed a surprisingly high tolerance against ionizing radiation, and two species (Aquifex pyrophilus and Ignicoccus hospitalis) were even able to survive 20 kGy. Therefore, these species had a comparable survivability after exposure to ionizing radiation
such as Deinococcus radiodurans. In contrast, there was nearly no difference in survival of the tested strains after exposure to UV-C under anoxic conditions.
If the cells had been dried in advance of UV-C irradiation, they were more sensitive to UV-C radiation compared with cells
irradiated in liquid suspension; this effect could be reversed by the addition of protective material like sulfidic ores before
irradiation. By exposure to UV-C, photoproducts were formed in the DNA of irradiated Archaea and Bacteria. The distribution
of the main photoproducts was species specific, but the amount of the photoproducts was only partly dependent on the applied
fluence. Overall, our results show that tolerance to radiation seems to be a common phenomenon among thermophilic and hyperthermophilic
microorganisms. 相似文献
997.
William Coley Rachel Van Duyne Lawrence Carpio Irene Guendel Kylene Kehn-Hall Sebastien Chevalier Aarthi Narayanan Truong Luu Norman Lee Zachary Klase Fatah Kashanchi 《The Journal of biological chemistry》2010,285(42):31930-31943
MicroRNAs (miRNAs) are a class of small RNA molecules that function to control gene expression and restrict viral replication in host cells. The production of miRNAs is believed to be dependent upon the DICER enzyme. Available evidence suggests that in T lymphocytes, HIV-1 can both suppress and co-opt the host''s miRNA pathway for its own benefit. In this study, we examined the state of miRNA production in monocytes and macrophages as well as the consequences of viral infection upon the production of miRNA. Monocytes in general express low amounts of miRNA-related proteins, and DICER in particular could not be detected until after monocytes were differentiated into macrophages. In the case where HIV-1 was present prior to differentiation, the expression of DICER was suppressed. MicroRNA chip results for RNA isolated from transfected and treated cells indicated that a drop in miRNA production coincided with DICER protein suppression in macrophages. We found that the expression of DICER in monocytes is restricted by miR-106a, but HIV-1 suppressed DICER expression via the viral gene Vpr. Additionally, analysis of miRNA expression in monocytes and macrophages revealed evidence that some miRNAs can be processed by both DICER and PIWIL4. Results presented here have implications for both the pathology of viral infections in macrophages and the biogenesis of miRNAs. First, HIV-1 suppresses the expression and function of DICER in macrophages via a previously unknown mechanism. Second, the presence of miRNAs in monocytes lacking DICER indicates that some miRNAs can be generated by proteins other than DICER. 相似文献
998.
Kyle Lapham Mark N. Kvale Jue Lin Sheryl Connell Lisa A. Croen Brad P. Dispensa Lynn Fang Stephanie Hesselson Thomas J. Hoffmann Carlos Iribarren Eric Jorgenson Lawrence H. Kushi Dana Ludwig Tetsuya Matsuguchi William B. McGuire Sunita Miles Charles P. Quesenberry Jr. Sarah Rowell Marianne Sadler Lori C. Sakoda David Smethurst Carol P. Somkin Stephen K. Van Den Eeden Lawrence Walter Rachel A. Whitmer Pui-Yan Kwok Neil Risch Catherine Schaefer Elizabeth H. Blackburn 《Genetics》2015,200(4):1061-1072
The Kaiser Permanente Research Program on Genes, Environment, and Health (RPGEH) Genetic Epidemiology Research on Adult Health and Aging (GERA) cohort includes DNA specimens extracted from saliva samples of 110,266 individuals. Because of its relationship to aging, telomere length measurement was considered an important biomarker to develop on these subjects. To assay relative telomere length (TL) on this large cohort over a short time period, we created a novel high throughput robotic system for TL analysis and informatics. Samples were run in triplicate, along with control samples, in a randomized design. As part of quality control, we determined the within-sample variability and employed thresholds for the elimination of outlying measurements. Of 106,902 samples assayed, 105,539 (98.7%) passed all quality control (QC) measures. As expected, TL in general showed a decline with age and a sex difference. While telomeres showed a negative correlation with age up to 75 years, in those older than 75 years, age positively correlated with longer telomeres, indicative of an association of longer telomeres with more years of survival in those older than 75. Furthermore, while females in general had longer telomeres than males, this difference was significant only for those older than age 50. An additional novel finding was that the variance of TL between individuals increased with age. This study establishes reliable assay and analysis methodologies for measurement of TL in large, population-based human studies. The GERA cohort represents the largest currently available such resource, linked to comprehensive electronic health and genotype data for analysis. 相似文献
999.
Laura A.B. Wilson Rachel Ives Hugo F.V. Cardoso Louise T. Humphrey 《American journal of physical anthropology》2015,156(1):19-34
Morphological traits of the ilium have consistently been more successful for juvenile sex determination than have techniques applied to other skeletal elements, however relatively little is known about the ontogeny and maturation of size and shape dimorphism in the ilium. We use a geometric morphometric approach to quantitatively separate the ontogeny of size and shape of the ilium, and analyze interpopulation differences in the onset, rate and patterning of sexual dimorphism. We captured the shape of three traits for a total of 191 ilia from Lisbon (Portugal) and London (UK) samples of known age and sex (0–17 years). Our results indicate that a) there is a clear dissociation between the ontogeny of size and shape in males and females, b) the ontogeny of size and shape are each defined by non‐linear trajectories that differ between the sexes, c) there are interpopulation differences in ontogenetic shape trajectories, which point to population‐specific patterning in the attainment of sexual dimorphism, and d) the rate of shape maturation and size maturation is typically higher for females than males. Male and female shape differences in the ilium are brought about by trajectory divergence. Differences in size and shape maturation between the sexes suggest that maturity may confound our ability to discriminate between the sexes by introducing variation not accounted for in age‐based groupings. The accuracy of sex determination methods using the ilium may be improved by the use of different traits for particular age groups, to capture the ontogenetic development of shape in both sexes. Am J Phys Anthropol 156:19–34, 2015 © 2014 Wiley Periodicals, Inc. 相似文献
1000.
Vector-borne diseases are of global importance to human and animal health. Empirical trials of effective methods to control
vectors and their pathogens can be difficult for practical, financial and ethical reasons. Here, therefore, we use a mathematical
model to predict the effectiveness of a vector-borne disease control method. As a case study, we use the tick-louping ill
virus system, where sheep are treated with acaricide in an attempt to control ticks and disease in red grouse , an economically
important game bird. We ran the model under different scenarios of sheep flock sizes, alternative host (deer) densities, acaricide
efficacies and tick burdens. The model predicted that, with very low deer densities, using sheep as tick mops can reduce the
tick population and virus prevalence. However, treatment is ineffective above a certain threshold deer density, dependent
on the comparative tick burden on sheep and deer. The model also predicted that high efficacy levels of acaricide must be
maintained for effective tick control. This study suggests that benignly managing one host species to protect another host
species from a vector and pathogen can be effective under certain conditions. It also highlights the importance of understanding
the ecological complexity of a system, in order to target control methods only under certain circumstances for maximum effectiveness. 相似文献