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排序方式: 共有66条查询结果,搜索用时 15 毫秒
41.
Sabri Denden Ramzi Lakhdar Nadia Leban Jemni Ben Chibani Amel Haj Khelil 《Molecular biotechnology》2010,45(2):111-115
Alpha 1 antitrypsin deficiency (AATD) is a well recognized genetic risk factor for pulmonary disease and less common liver
disease. The two most common deficiency alleles worldwide PI*S and PI*Z can be easily detected using several molecular methods.
However, there are at least 30 other AATD variants, which are only detectable by alpha 1 antitrypsin (AAT) gene sequencing
and, therefore, seem to be more under-recognized than the PI*S and PI*Z alleles. PI*Mmalton is the most frequent AATD variant
in different regions of the Southern Mediterranean basin countries, where its prevalence seems to prevail over PI*S and PI*Z.
In this work, we report the development of a simple PCR-based analysis designed for the detection of the PI*Mmalton deficiency
alleles using two specific primers. A one-tube reaction enables the distinction between the different genotypes. This reliable,
easy, fast, and low-cost technique might be useful for laboratories involved in the study of AATD-related diseases, especially
those of the Southern Mediterranean basin area with modest budget or where sophisticated equipment is not available. This
will allow larger targeted screening for PI*Mmalton in order to better understand this mutation epidemiology and its origin. 相似文献
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43.
Souad Zaim Ahmed Amine Bekkar Lakhdar Belabid 《Archives Of Phytopathology And Plant Protection》2018,51(3-4):217-226
Fusarium wilt is caused by F. oxysporum Schlecht end. Fr. f. sp. ciceris (FOC) is a devastating disease of chickpea in Algeria. In this study, antagonistic effects of B. subtilis MF352017 (Bs1) and Trichoderma harzianum KX523899 (T5) isolated from the rhizosphere of chickpea were investigated separately and in combination for their efficacy in controlling the disease in vivo. The efficacy of the antagonistic biocontrol agents on Fusarium wilt was evaluated based on vegetative and root growth parameters of chickpea. Seed bacterisation with B. subtilis MF352017 (Bs1) and seed treatment with T. harzianum (T5) significantly protected chickpea seedlings from FOC as compared to untreated plants. Plant protection was more pronounced in T. harzianum-treated plants than in bacterised plants. The application of both antagonists effectively suppressed 93.67% of the disease and also enhanced plant growth leading to increased plant height, root length, fresh and dry weights of shoot and root. The mixture of antagonists increased the effectiveness of B. subtilis MF352017 (Bs1) isolate on Fusarium wilt and improved chickpea growth. 相似文献
44.
Biopharmaceutical companies with large portfolios of clinical and commercial products typically need to allocate production across several multiproduct facilities, including third party contract manufacturers. This poses several capacity planning challenges which are further complicated by the need to satisfy different stakeholders often with conflicting objectives. This work addresses the question of how a biopharmaceutical manufacturer can make better long-term capacity planning decisions given multiple strategic criteria such as cost, risk, customer service level, and capacity utilization targets. A long-term planning model that allows for multiple facilities and accounts for multiple objectives via goal programming is developed. An industrial case study based on a large scale biopharmaceutical manufacturer is used to illustrate the functionality of the model. A single objective model is used to identify how best to use existing capacity so as to maximize profits for different demand scenarios. Mitigating risk due to unforeseen circumstances by including a dual facility constraint is shown to be a reasonable strategy at base case demand levels but unacceptable if demands are 150% higher than expected. The capacity analysis identifies where existing capacity fails to meet demands given the constraints. A multiobjective model is used to demonstrate how key performance measures change given different decision making policies where different weights are assigned to cost, customer service level, and utilization targets. The analysis demonstrates that a high profit can still be achieved while meeting key targets more closely. The sensitivity of the optimal solution to different limits on the targets is illustrated. 相似文献
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Denden S Khelil AH Knani J Lakhdar R Perrin P Lefranc G Chibani JB 《Genetics and molecular biology》2010,33(1):23-26
Alpha-1-antitrypsin (AAT) plays an important role in the pathogenesis of emphysema, the pathological lesion underlying the majority of the manifestations of Chronic Obstructive Pulmonary Disease (COPD). In this study we tested the hypothesis that common AAT polymorphisms influence the risk of developing COPDs. We investigated PiM1 (Ala213Val), PiM2 (Arg101His), PiM3 (Glu376Asp), PiS (Glu264Val) and PiZ (Glu342Lys) SERPINA1 alleles in 100 COPD patients and 200 healthy controls. No significant differences were observed in allele frequencies between COPD patients and controls, neither did haplotype analysis show significant differences between the two groups. A cross-sectional study revealed no significant relationship between common SERPINA1 polymorphisms (PiM1, PiM2, PiM3) and the emphysematous type of COPD. In addition, FEV(1) annual decline, determined during a two-year follow up period, revealed no difference among carriers of the tested polymorphisms. 相似文献
48.
Carolina Agop-Nersesian Bernina Naissant Fathia Ben Rached Manuel Rauch Angelika Kretzschmar Sabine Thiberge Robert Menard David J. P. Ferguson Markus Meissner Gordon Langsley 《PLoS pathogens》2009,5(1)
The final step during cell division is the separation of daughter cells, a process that requires the coordinated delivery and assembly of new membrane to the cleavage furrow. While most eukaryotic cells replicate by binary fission, replication of apicomplexan parasites involves the assembly of daughters (merozoites/tachyzoites) within the mother cell, using the so-called Inner Membrane Complex (IMC) as a scaffold. After de novo synthesis of the IMC and biogenesis or segregation of new organelles, daughters bud out of the mother cell to invade new host cells. Here, we demonstrate that the final step in parasite cell division involves delivery of new plasma membrane to the daughter cells, in a process requiring functional Rab11A. Importantly, Rab11A can be found in association with Myosin-Tail-Interacting-Protein (MTIP), also known as Myosin Light Chain 1 (MLC1), a member of a 4-protein motor complex called the glideosome that is known to be crucial for parasite invasion of host cells. Ablation of Rab11A function results in daughter parasites having an incompletely formed IMC that leads to a block at a late stage of cell division. A similar defect is observed upon inducible expression of a myosin A tail-only mutant. We propose a model where Rab11A-mediated vesicular traffic driven by an MTIP-Myosin motor is necessary for IMC maturation and to deliver new plasma membrane to daughter cells in order to complete cell division. 相似文献
49.
Ramzi Lakhdar Sabri Denden Jalel Knani Nadia Leban Houria Daimi Mohsen Hassine Gérard Lefranc Jemni Ben Chibani Amel Haj Khelil 《Biochemical genetics》2010,48(7-8):647-657
GSTM1 and GSTT1 polymorphisms have been proposed in relationship with chronic obstructive pulmonary disease (COPD). We investigated the association between these polymorphisms and COPD (as well as its subtypes emphysema and chronic bronchitis) in 234 COPD patients and 182 healthy controls in the Tunisian population. Genotyping was performed using multiplex PCR. GSTM1-null genotype frequency was significantly higher in COPD patients than in controls (P = 0.02); however, multivariate analysis of cofounding variables showed no independent association with this genotype (P = 0.073). In contrast, the association of the GSTM1-null genotype with emphysema was significant, even after adjustment for risk factors (P = 0.011). There were no significant differences in GSTT1 genotypes between patients and controls. The GSTM1 null allele is likely not an independent risk factor for COPD but is related to emphysema, whereas the GSTT1 gene is not associated with the disease. 相似文献
50.