全文获取类型
收费全文 | 864篇 |
免费 | 97篇 |
专业分类
961篇 |
出版年
2023年 | 11篇 |
2022年 | 17篇 |
2021年 | 34篇 |
2020年 | 18篇 |
2019年 | 20篇 |
2018年 | 31篇 |
2017年 | 26篇 |
2016年 | 41篇 |
2015年 | 54篇 |
2014年 | 63篇 |
2013年 | 54篇 |
2012年 | 102篇 |
2011年 | 81篇 |
2010年 | 41篇 |
2009年 | 41篇 |
2008年 | 46篇 |
2007年 | 54篇 |
2006年 | 48篇 |
2005年 | 44篇 |
2004年 | 27篇 |
2003年 | 27篇 |
2002年 | 25篇 |
2001年 | 5篇 |
2000年 | 6篇 |
1999年 | 7篇 |
1998年 | 9篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 6篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有961条查询结果,搜索用时 0 毫秒
941.
Remko Leijs Tessa Bradford James G. Mitchell William F. Humphreys Steven J. B. Cooper Peter Goonan Rachael A. King 《PloS one》2015,10(8)
Three new species of Koonunga were discovered in surface and subterranean waters in southern Australia, and were defined using mtDNA analyses and morphology. The new species are: Koonunga hornei Leijs & King; K. tatiaraensis Leijs & King and K. allambiensis Leijs & King. Molecular clock analyses indicate that the divergence times of the species are older than the landscape that they currently inhabit. Different scenarios explaining this apparent discrepancy are discussed in the context of the palaeography of the area. A freshwater epigean origin for Koonunga is considered the most likely hypothesis, whereby some lineages made the transition to the subterranean environment within the last few million years influenced by significant climatic cooling/drying. We discuss the possibility that one stygobitic lineage secondarily regained some of its body pigmentation as adaptation to increased photic conditions after cave collapse and forming of cenotes during the last glacial maximum. 相似文献
942.
943.
State I-State II transitions were monitored in vivo and in vitro in the Antarctic, psychrophillic, green alga, Chlamydomonas subcaudata, as changes in the low-temperature (77 K) chlorophyll fluorescence emission maxima at 722 nm (F722) relative to 699 nm (F699). As expected, the control mesophillic species, Chlamydomonas reinhardtii, was able to modulate the light energy distribution between photosystem II and photosystem I in response to exposure to four different conditions: (i) dark/anaerobic conditions, (ii) a change in Mg2+ concentration, (iii) red light, and (iv) increased incubation temperature. This was correlated with the ability to phosphorylate both of its major light-harvesting polypeptides. In contrast, exposure of C. subcaudata to the same four conditions induced minimum alterations in the 77 K fluorescence emission spectra, which was correlated with the ability to phosphorylate only one of its major light-harvesting polypeptides. Thus, C. subcaudata appears to be deficient in the ability to undergo a State I-State II transition. Functionally, this is associated with alterations in the apparent redox status of the intersystem electron transport chain and with higher rates of photosystem I cyclic electron transport in the psychrophile than in the mesophile, based on in vivo P700 measurements. Structurally, this deficiency is associated with reduced levels of Psa A/B relative to D1, the absence of specific photosystem I light-harvesting polypeptides [R.M. Morgan et al. (1998) Photosynth Res 56:303-314] and a cytochrome b6/f complex that exhibits a form of cytochrome f that is approximately 7 kDa smaller than that observed in C. reinhardtii. We conclude that the Antarctic psychrophile, C. subcaudata, is an example of a natural variant deficient in State I-State II transitions. 相似文献
944.
Eleonora Chiri Chris Greening Rachael Lappan David W. Waite Thanavit Jirapanjawat Xiyang Dong Stefan K. Arndt Philipp A. Nauer 《The ISME journal》2020,14(11):2715
Termite mounds have recently been confirmed to mitigate approximately half of termite methane (CH4) emissions, but the aerobic CH4 oxidising bacteria (methanotrophs) responsible for this consumption have not been resolved. Here, we describe the abundance, composition and CH4 oxidation kinetics of the methanotroph communities in the mounds of three distinct termite species sampled from Northern Australia. Results from three independent methods employed show that methanotrophs are rare members of microbial communities in termite mounds, with a comparable abundance but distinct composition to those of adjoining soil samples. Across all mounds, the most abundant and prevalent methane monooxygenase sequences were affiliated with upland soil cluster α (USCα), with sequences homologous to Methylocystis and tropical upland soil cluster (TUSC) also detected. The reconstruction of a metagenome-assembled genome of a mound USCα representative highlighted the metabolic capabilities of this group of methanotrophs. The apparent Michaelis–Menten kinetics of CH4 oxidation in mounds were estimated from in situ reaction rates. Methane affinities of the communities were in the low micromolar range, which is one to two orders of magnitude higher than those of upland soils, but significantly lower than those measured in soils with a large CH4 source such as landfill cover soils. The rate constant of CH4 oxidation, as well as the porosity of the mound material, were significantly positively correlated with the abundance of methanotroph communities of termite mounds. We conclude that termite-derived CH4 emissions have selected for distinct methanotroph communities that are kinetically adapted to elevated CH4 concentrations. However, factors other than substrate concentration appear to limit methanotroph abundance and hence these bacteria only partially mitigate termite-derived CH4 emissions. Our results also highlight the predominant role of USCα in an environment with elevated CH4 concentrations and suggest a higher functional diversity within this group than previously recognised.Subject terms: Soil microbiology, Biogeochemistry 相似文献
945.
946.
Lucas T. Graybuck Tanya L. Daigle Adriana E. Sedeño-Cortés Miranda Walker Brian Kalmbach Garreck H. Lenz Elyse Morin Thuc Nghi Nguyen Emma Garren Jacqueline L. Bendrick Tae Kyung Kim Thomas Zhou Marty Mortrud Shenqin Yao La’ Akea Siverts Rachael Larsen Bryan B. Gore Eric R. Szelenyi Bosiljka Tasic 《Neuron》2021,109(9):1449-1464.e13
947.
Monica Sueiro-Olivares Jennifer Scott Sara Gago Dunja Petrovic Emilia Kouroussis Jasmina Zivanovic Yidong Yu Marlene Strobel Cristina Cunha Darren Thomson Rachael Fortune-Grant Sina Thusek Paul Bowyer Andreas Beilhack Agostinho Carvalho Elaine Bignell Milos R. Filipovic Jorge Amich 《PLoS biology》2021,19(6)
Aspergillus fumigatus is a human fungal pathogen that can cause devastating pulmonary infections, termed “aspergilloses,” in individuals suffering immune imbalances or underlying lung conditions. As rapid adaptation to stress is crucial for the outcome of the host–pathogen interplay, here we investigated the role of the versatile posttranslational modification (PTM) persulfidation for both fungal virulence and antifungal host defense. We show that an A. fumigatus mutant with low persulfidation levels is more susceptible to host-mediated killing and displays reduced virulence in murine models of infection. Additionally, we found that a single nucleotide polymorphism (SNP) in the human gene encoding cystathionine γ-lyase (CTH) causes a reduction in cellular persulfidation and correlates with a predisposition of hematopoietic stem cell transplant recipients to invasive pulmonary aspergillosis (IPA), as correct levels of persulfidation are required for optimal antifungal activity of recipients’ lung resident host cells. Importantly, the levels of host persulfidation determine the levels of fungal persulfidation, ultimately reflecting a host–pathogen functional correlation and highlighting a potential new therapeutic target for the treatment of aspergillosis.This study reveals that the post-translational modification persulfidation is important for both fungal virulence and the host antifungal response. The level of persulfidation in the host, which correlates with its antifungal potency, impacts the level required in the fungus to counteract host attack, reflecting a functional correlation. Thus modulating persulfidation may be a promising strategy to target both pathogens and immune responses. 相似文献
948.
949.
A northward shift of range margins in British Odonata 总被引:4,自引:0,他引:4
Rachael Hickling † David B. Roy Jane K. Hill† Chris D. Thomas† 《Global Change Biology》2005,11(3):502-506
Many species are predicted to shift their ranges to higher latitudes and altitudes in response to climate warming. This study presents evidence for 37 species of nonmigratory British dragonflies and damselflies shifting northwards at their range margins over the past 40 years, seemingly as a result of climate change. This response by an exemplar group of insects associated with fresh water, parallels polewards range changes observed in terrestrial invertebrates and other taxa. 相似文献
950.
Egg hatching times of Antarctic copepods 总被引:2,自引:0,他引:2
Egg hatching times were determined at a range of temperatures for four species of commonly occurring Antarctic copepods.
At a given temperature the eggs of Rhincalanus gigas took longest to hatch, up to 9 days at 0°C, followed by those of Calanoides acutus, Calanus propinquus and Calanus simillimus. A Bělehrádeks temperature function with the parameter b fixed at −2.05 accounted for >95% of the variance for each species. There was an approximate doubling in hatching times between
5°C and 0°C for R. gigas and for the other species the increase in embryonic duration was 40–50% at the lower temperature.
Received: 27 March 1997 / Accepted: 17 August 1997 相似文献