全文获取类型
收费全文 | 23580篇 |
免费 | 1525篇 |
国内免费 | 6篇 |
出版年
2023年 | 179篇 |
2022年 | 177篇 |
2021年 | 370篇 |
2020年 | 325篇 |
2019年 | 340篇 |
2018年 | 681篇 |
2017年 | 603篇 |
2016年 | 828篇 |
2015年 | 1266篇 |
2014年 | 1309篇 |
2013年 | 1673篇 |
2012年 | 1934篇 |
2011年 | 1785篇 |
2010年 | 1114篇 |
2009年 | 846篇 |
2008年 | 1399篇 |
2007年 | 1320篇 |
2006年 | 1186篇 |
2005年 | 1081篇 |
2004年 | 1020篇 |
2003年 | 926篇 |
2002年 | 831篇 |
2001年 | 401篇 |
2000年 | 331篇 |
1999年 | 273篇 |
1998年 | 171篇 |
1997年 | 125篇 |
1996年 | 123篇 |
1995年 | 128篇 |
1994年 | 88篇 |
1993年 | 95篇 |
1992年 | 163篇 |
1991年 | 134篇 |
1990年 | 134篇 |
1989年 | 113篇 |
1988年 | 98篇 |
1987年 | 98篇 |
1986年 | 79篇 |
1985年 | 118篇 |
1984年 | 96篇 |
1983年 | 63篇 |
1982年 | 67篇 |
1981年 | 80篇 |
1980年 | 57篇 |
1979年 | 85篇 |
1978年 | 87篇 |
1977年 | 61篇 |
1974年 | 73篇 |
1973年 | 61篇 |
1971年 | 62篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
941.
Plants can interact with other plants through the release of chemical compounds or allelochemicals. These compounds released by donor plants influence germination, growth, development, and establishment of receptor plants; having an important role on the pattern of vegetation, i.e as invasive strategy, and on crop productivity. This phytotoxic or negative effect of the released allelochemicals (allelochemical stress) is caused by modifying or altering diverse metabolic processes, having many molecular targets in the receptor plants. Recently, using an aggressive and allelopathic plant Sicyos deppei as the donor plant, and Lycopersicon esculentum as the receptor plant, we showed that the allelochemicals released by S. deppei caused oxidative damage through an increase in reactive oxygen species (ROS) and activation or modification of antioxidant enzymes. Based on this study, we proposed that oxidative stress is one of the mechanisms, among others, by which an allelopathic plant causes phytotoxicity to other plants.Key Words: allelochemical stress, Sicyos deppei, Lycopersicon esculentum, plant allelochemicals, phytotoxicity, ROS, lipid peroxidationIt is well known that plants interact with many organisms, including co-habitation with other plants. Among these relations are the ones referred to as allelochemical interactions. Allelopathy can be defined as a mechanism of interference in plant growth and development mediated by the addition of plant-produced secondary products (allelochemicals) to the soil rhizosphere. Allelochemicals are present in all types of plants and tissues and are released into the soil rhizosphere by a variety of mechanisms, including decomposition of residues, volatilization, and root exudation.1–3 These released allelochemicals become stressful only when they are toxic or when they affect the growth and development of surrounding plants (phytotoxicity). Studies on allelochemical stress have been expanding; recently the phenomenon has taken on increased importance, since it can help explain plant growth inhibition in interspecies interactions and in structuring the plant community. It appears to be one mechanism or strategy used by invasive plants to become successful and replace other native ones.4–6On the other hand, the chemical diversity of the organic compounds that mediate these allelochemical interactions is as diverse as their modes of action. Many studies have shown that allelochemicals interfere with several physiological processes in the receptor organism.3,7,8 The physiological effects on receptor plants or other organisms are useful in determining the role of the allelochemicals in the system. Recently, it has been proposed that allelochemicals can cause oxidative stress in target plants and therefore activate the antioxidant mechanism.3,8–12 In particular; our studies have been focused on knowing the physiological targets of the phytotoxic compounds released by a noxious and endemic weed Sicyos deppei G. Don (Cucurbitaceae). We have taken as the model the receptor or damaged plant Lycopersicon esculentum Mill (Solanaceae), since in Mexican crop-fields, it is common to find both plants. We have observed the strong allelopathic potential of S. deppei and are exploring the potential metabolic target that could be involved in the strong phytotoxic effect of this weed.13–16 We recently documented the oxidative damage that an aqueous leachate of S. deppei caused in the target plant L. esculentum.16 In this work we explored in seeds and in primary roots the antioxidant mechanism of tomato to determine whether or not the inhibitory effect of S. deppei was due to oxidative damage. We analyzed the activity and expression of some antioxidant enzymes involved in the detoxification of ROS, and found an imbalance in its activity as well as an increase in the levels of H2O2 at 24 h of treatment. Additional studies on the levels of ROS, including hydrogen peroxide, were monitored in primary roots from germinating seeds under allelochemical stress by imaging the ROS-sensitive fluorescent dye dichlorofluorescein (H2DCFDA, carboxy-2′, 7′-diclhlorofluorescein diacetate) in a confocal microscope (BIORAD 1024, 488 nm dichroic and 510–560 nm emission). DCFDA fluorescence increases as the dye is oxidized by ROS to dichlorofluorescein (DCF). Figure 1 shows a marked increase in fluorescence at 48 h and 72 h of treatment (Fig. 1A–C) compared with the same treatment at 24 h, and with the corresponding control. This fluorescence was more evident at the root cap and at the zone of root hairs in treated seeds.Open in a separate windowFigure 1Allelochemical stress caused by S. deppei elicits ROS generation in tomato germinating seeds. Panels show control (left) and treatment (right) at 24 h (A), 48 h (B), and 72 h (C). Lower panels show higher magnification (40X) of the corresponding time. Seedlings with primary roots were stained for 10–15 minutes with 25 µM DCFDA in distilled water.Clearly, allelochemical stress caused by S. deppei is producing an oxidative imbalance as evidenced by generation of ROS and alteration of activity of antioxidant enzymes. Another result that supports this observation is the high level of lipid peroxidation that we observed at 48 and 72 h, which correlates with the inhibition of two membrane-associated enzymes, H+-ATPase15 and NADPH oxidase.16 We believe, however, that the oxidative damage we observed is not solely responsible for the phytotoxic effect of S. deppei on tomato growth. In other words, we suggest that its inhibitory effect represents the sum of many metabolic processes affected at different times. Currently we are studying the dynamics of carbohydrate mobilization, cell wall loosing of the endosperm to allow the protrusion of the radicle, and ABA content. Preliminary results have shown that there is a delay in expression of some enzyme activities and a high content of ABA. 相似文献
942.
Sarah Noël Martin Ouellet Patrick Galois François-Joseph Lapointe 《Conservation Genetics》2007,8(3):599-606
Urban development is a major cause of habitat loss and fragmentation. Few studies, however, have dealt with fragmentation
in an urban landscape. In this paper, we examine the genetic structure of isolated populations of the eastern red-backed salamander
(Plethodon cinereus) in a metropolitan area. We sampled four populations located on a mountain in the heart of Montréal (Québec, Canada), which
presents a mosaic of forested patches isolated by roads, graveyards and buildings. We assessed the genetic structure of these
populations using microsatellite loci and compared it to the genetic structure of four populations located in a continuous
habitat in southern Québec. Our results indicate that allelic richness and heterozygosity are lower in the urban populations.
Exact differentiation tests and pairwise F
ST also show that the populations found in the fragmented habitat are genetically differentiated, whereas populations located
in the continuous habitat are genetically homogeneous. These results raise conservation concerns for these populations as
well as for rare or threatened species inhabiting urban landscapes. 相似文献
943.
Santos LC Ramos EA Gurgel CA de Santana EJ Dos Santos JN 《Journal of molecular histology》2007,38(3):201-205
Objective Dental granulomas (DGs) and radicular cysts (RCs) are chronic periapical lesions frequently involving the jaws. Langerhans
cells (LCs) are dendritic cells responsible for the presentation of antigens to T lymphocytes. This study examined the expression
of LCs in DG and RCs by immunohistochemical staining.
Study Design Eighteen cases of DGs and 26 cases of RCs were analyzed using anti-CD1a marker.
Results CD1a-labeled LCs were observed in 11.1% of DGs and in 69.2% of RCs, showing a significant correlation (P < 0.0001; Fisher’s test). In DGs, LCs were only observed in granulation tissue, showing discrete immunostaining density.
In RCs, LCs exhibited both a round and a dendritic shape in all epithelial layers. Although a correlation was observed between
immunostaining density and epithelial thickness, as well as between immunostaining and inflammatory intensity, the differences
were not significant in radicular cysts.
Conclusion Langerhans cells provide important insight into the immunopathogenesis of chronic periapical lesions. 相似文献
944.
Madoz-Gúrpide J Cañamero M Sanchez L Solano J Alfonso P Casal JI 《Molecular & cellular proteomics : MCP》2007,6(12):2150-2164
To gain further insight into alterations in cellular pathways, tumor profiling, and marker discovery in colorectal cancer (CRC) we used a new antibody microarray specific for cell signaling. Soluble protein extracts were prepared from paired tumor/normal biopsies of 11 patients diagnosed with colorectal carcinoma at different stages; four liver carcinomas were used as a reference. Antibody microarray analysis identified 46 proteins that were differentially expressed between normal colorectal epithelium and adenocarcinoma. These proteins gave a specific signature for CRC, different from other tumors, as well as a panel of novel markers and potential targets for CRC. Twenty-four proteins were validated by using a specific colorectal cancer tissue microarray and immunoblotting analysis. Together with some previously well known deregulated proteins in CRC (beta-catenin, c-MYC, or p63), we found new potential markers preferentially expressed in CRC tumors: cytokeratin 13, calcineurin, CHK1, clathrin light chain, MAPK3, phospho-PTK2/focal adhesion kinase (Ser-910), and MDM2. CHK1 antibodies were particularly effective in discriminating between tumoral and normal mucosa in CRC. Moreover a global picture of alterations in signaling pathways in CRC was observed, including a significant up-regulation of different components of the epidermal growth factor receptor and Wnt/beta-catenin pathways and the down-regulation of p14(ARF). The experimental approach described here should be applicable to other pathologies and neoplastic processes. 相似文献
945.
Marmagne A Ferro M Meinnel T Bruley C Kuhn L Garin J Barbier-Brygoo H Ephritikhine G 《Molecular & cellular proteomics : MCP》2007,6(11):1980-1996
The proteomics of plasma membrane has brought to date only scarce and partial information on the actual protein repertoire. In this work, the plant plasma membrane proteome of Arabidopsis thaliana was investigated. A highly purified plasma membrane fraction was washed by NaCl and Na2CO3 salts, and the insoluble fractions were further analyzed by nano-LC-MS/MS. With 446 proteins identified, we hereby describe the largest plasma membrane proteome diversity reported so far. Half of the proteins were predicted to display transmembrane domains and/or to be anchored to the membrane, validating a posteriori the pertinence of the approach. A fine analysis highlighted two main specific and novel features. First, the main functional category is represented by a majority of as yet unreported signaling proteins, including 11% receptor-like kinases. Second, 16% of the identified proteins are predicted to be lipid-modified, specifically involving double lipid linkage through N-terminal myristoylation, S-palmitoylation, C-terminal prenylation, or glycosylphosphatidylinositol anchors. Thus, our approach led for the first time to the identification of a large number of peripheral proteins as part of the plasma membrane and allowed the functionality of the plasma membrane in the cell context to be reconsidered. 相似文献
946.
947.
Glutathione peroxidases (GPXs, EC 1.11.1.9) were first discovered in mammals as key enzymes involved in scavenging of activated oxygen species (AOS). Their efficient antioxidant activity depends on the presence of the rare amino-acid residue selenocysteine (SeCys) at the catalytic site. Nonselenium GPX-like proteins (NS-GPXs) with a Cys residue instead of SeCys have also been found in most organisms. As SeCys is important for GPX activity, the function of the NS-GPX can be questioned. Here, we highlight the evolutionary link between NS-GPX and seleno-GPX, particularly the evolution of the SeCys incorporation system. We then discuss what is known about the enzymatic activity and physiological functions of NS-GPX. Biochemical studies have shown that NS-GPXs are not true GPXs; notably they reduce AOS using reducing substrates other than glutathione, such as thioredoxin. We provide evidence that, in addition to their inefficient scavenging action, NS-GPXs act as AOS sensors in various signal-transduction pathways. 相似文献
948.
A single bout of exercise with high mechanical loading induces the expression of Cyr61/CCN1 and CTGF/CCN2 in human skeletal muscle. 总被引:1,自引:0,他引:1
Riikka Kivel? Heikki Kyr?l?inen Harri Sel?nne Paavo V Komi Heikki Kainulainen Veikko Vihko 《Journal of applied physiology》2007,103(4):1395-1401
High mechanical loading was hypothesized to induce the expression of angiogenic and/or lymphangiogenic extracellular matrix (ECM) proteins in skeletal muscle. Eight men performed a strenuous exercise protocol, which consisted of 100 unilateral maximal drop jumps followed by submaximal jumping until exhaustion. Muscle biopsies were taken 30 min and 48 h postexercise from the vastus lateralis muscle and analyzed for the following parameters: mRNA and protein expression of ECM-associated CCN proteins [cysteine-rich angiogenic protein 61 (Cyr61)/CCN1, connective tissue growth factor (CTGF)/CCN2], and mRNA expression of vascular endothelial growth factors (VEGFs) and hypoxia-inducible factor-1alpha. The mRNA expression of Cyr61 and CTGF increased 30 min after the exercise (14- and 2.5-fold, respectively; P < 0.001). Cyr61 remained elevated 48 h postexercise (threefold; P < 0.05). The mRNA levels of VEGF-A, VEGF-B, VEGF-C, VEGF-D, or hypoxia-inducible factor-1alpha did not change significantly at either 30 min or 48 h postexercise; however, the variation between subjects increased markedly in VEGF-A and VEGF-B mRNA. Cyr61 protein levels were higher at both 30 min and 48 h after the exercise compared with the control (P < 0.05). Cyr61 and CTGF proteins were localized to muscle fibers and the surrounding ECM by immunohistochemistry. Fast fibers stained more intensively than slow fibers. In conclusion, mechanical loading induces rapid expression of CCN proteins in human skeletal muscle. This may be one of the early mechanisms involved in skeletal muscle remodeling after exercise, since Cyr61 and CTGF regulate the expression of genes involved in angiogenesis and ECM remodeling. 相似文献
949.
Sunmin Park Sang Mee Hong Ji Eun Lee So Ra Sung 《Journal of applied physiology》2007,103(5):1764-1771
In this study, we investigated the effects of a high-fat diet and exercise on pancreatic beta-cell function and mass and its molecular mechanism in 90% pancreatectomized male rats. The pancreatectomized diabetic rats were given control diets (20% energy) or a high-fat (HF) diet (45% energy) for 12 wk. Half of each group was given regular exercise on an uphill treadmill at 20 m/min for 30 min 5 days/wk. HF diet lowered first-phase insulin secretion with glucose loading, whereas exercise training reversed this decrease. However, second-phase insulin secretion did not differ among the groups. Exercise increased pancreatic beta-cell mass. This resulted from stimulated beta-cell proliferation and reduced apoptosis, which is associated with potentiated insulin or IGF-I signaling through insulin receptor substrate-2 (IRS2) induction. Although the HF diet resulted in decreased proliferation and accelerated apoptosis by weakened insulin and IGF-I signaling from reduction of IRS2 protein, beta-cell mass was maintained in HF rats just as much as in control rats via increased individual beta-cell size and neogenesis from precursor cells. Consistent with the results of beta-cell proliferation, pancreas duodenal homeobox-1 expression increased in the islets of rats in the exercise groups, and it was reduced the most in rats fed the HF diet. In conclusion, exercise combined with a moderate fat diet is a good way to maximize beta-cell function and mass through IRS2 induction to alleviate the diabetic condition. This study suggests that dietary fat contents and exercise modulate beta-cell function and mass to overcome insulin resistance in two different pathways. 相似文献