全文获取类型
收费全文 | 341篇 |
免费 | 40篇 |
专业分类
381篇 |
出版年
2018年 | 5篇 |
2015年 | 8篇 |
2014年 | 7篇 |
2013年 | 22篇 |
2012年 | 16篇 |
2011年 | 15篇 |
2010年 | 10篇 |
2009年 | 10篇 |
2008年 | 16篇 |
2007年 | 13篇 |
2006年 | 20篇 |
2005年 | 14篇 |
2004年 | 9篇 |
2003年 | 17篇 |
2002年 | 5篇 |
2001年 | 12篇 |
2000年 | 9篇 |
1999年 | 9篇 |
1997年 | 4篇 |
1996年 | 6篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 9篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 10篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1975年 | 3篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1971年 | 4篇 |
1969年 | 2篇 |
1968年 | 4篇 |
1967年 | 2篇 |
1965年 | 4篇 |
1964年 | 2篇 |
1963年 | 2篇 |
1961年 | 2篇 |
1948年 | 2篇 |
1889年 | 2篇 |
排序方式: 共有381条查询结果,搜索用时 15 毫秒
51.
52.
53.
A William Sheel Andrei V Krassioukov J Timothy Inglis Stacy L Elliott 《Journal of applied physiology》2005,99(1):53-58
Autonomic dysreflexia (AD) can occur during penile vibratory stimulation in men with spinal cord injury, but this is variable, and the association with lesion level is unclear. The purpose of this study was to characterize the cardiovascular responses to penile vibratory stimulation in men with spinal cord injury. We hypothesized that those with cervical injuries would demonstrate a greater degree of AD compared with men with thoracic injuries. We also questioned whether the rise in blood pressure could be attenuated by sildenafil citrate. Participants were classified as having cervical (n = 8) or thoracic (n = 5) injuries. While in a supine position, subjects were instrumented with an ECG, and arterial blood pressure was determined beat by beat. Subjects reported to the laboratory twice and received an oral dose of sildenafil citrate (25-100 mg) or no medication. Penile vibratory stimulation was performed using a handheld vibrator to the point of ejaculation. At ejaculation during the nonmedicated trials, the cervical group had a significant decrease in heart rate (-5-10 beats/min) and increase in mean arterial blood pressure (+70-90 mmHg) relative to resting conditions, whereas the thoracic group had significant increases in both heart rate (+8-15 beats/min) and mean arterial pressure (+25-30 mmHg). Sildenafil citrate had no effect on the change in heart rate or mean arterial pressure in either group. In summary, men with cervical injuries had more pronounced AD during penile vibratory stimulation than men with thoracic injuries. Administration of sildenafil citrate had no effect on heart rate or blood pressure during penile vibratory stimulation in men with spinal cord injury. 相似文献
54.
55.
Existing procedures for amide analysis of soluble proteins have been studied. Use of the automatic amino acid analyser for the determination of released ammonia gave greatly increased sensitivity over the distillation procedure and more reliable blank values. For the insoluble fibrous proteins, wool and silk hydrolysis in vacuo was found to remove the need for an extrapolation procedure; prolonged hydrolysis was required with hydrochloric acid but not with hydriodic acid. 相似文献
56.
Claire Z. Larter Chrystal Inglis Michael A. Silverman Geoffrey C. Farrell Michel R. Leroux 《Developmental neurobiology》2013,73(1):1-13
Primary cilia are ubiquitous cellular antennae whose dysfunction collectively causes various disorders, including vision and hearing impairment, as well as renal, skeletal, and central nervous system anomalies. One ciliopathy, Alström syndrome, is closely related to Bardet–Biedl syndrome (BBS), sharing amongst other phenotypic features morbid obesity. As the cellular and molecular links between weight regulation and cilia are poorly understood, we used the obese mouse strain foz/foz, bearing a truncating mutation in the Alström syndrome protein (Alms1), to help elucidate why it develops hyperphagia, leading to early onset obesity and metabolic anomalies. Our in vivo studies reveal that Alms1 localizes at the base of cilia in hypothalamic neurons, which are implicated in the control of satiety. Alms1 is lost from this location in foz/foz mice, coinciding with a strong postnatal reduction (~70%) in neurons displaying cilia marked with adenylyl cyclase 3 (AC3), a signaling protein implicated in obesity. Notably, the reduction in AC3‐bearing cilia parallels the decrease in cilia containing two appetite‐regulating proteins, Mchr1 and Sstr3, as well as another established Arl13b ciliary marker, consistent with progressive loss of cilia during development. Together, our results suggest that Alms1 maintains the function of neuronal cilia implicated in weight regulation by influencing the maintenance and/or stability of the organelle. Given that Mchr1 and Sstr3 localization to remaining cilia is maintained in foz/foz animals but known to be lost from BBS knockout mice, our findings suggest different molecular etiologies for the satiety defects associated with the Alström syndrome and BBS ciliopathies. © 2012 Wiley Periodicals, Inc. Develop Neurobiol, 2013 相似文献
57.
Peter W. Inglis Rubia B. C. Sarmento Camila F. C. Gavião M. Cléria Valadares-Inglis 《World journal of microbiology & biotechnology》2005,21(8-9):1487-1492
Summary Telomeric fingerprinting was found to be highly differentiating for Paecilomyces fumosoroseus and Paecilomyces lilacinus isolates in comparison to intron splice site PCR and is therefore a good method for quality control of future products based
on these fungi. Although the telomeric restriction length polymorphisms correctly divided the isolates into their appropriate
species, further correlation with host range or geographical origin of the isolates was not found. In this respect, intron
splice site PCR was more informative taxonomically. The chromosome numbers inferred from telomeric fingerprints were seven
chromosomes for P. lilacinus and between six and nine chromosomes for P. fumosoroseus. 相似文献
58.
Leblanc PJ Mulligan M Antolic A Macpherson L Inglis JG Martin D Roy BD Peters SJ 《American journal of physiology. Regulatory, integrative and comparative physiology》2008,295(4):R1224-R1230
Pyruvate dehydrogenase (PDH) plays an important role in regulating carbohydrate metabolism in skeletal muscle. PDH is activated by PDH phosphatase (PDP) and deactivated by PDH kinase (PDK). Obesity has a large negative impact on skeletal muscle carbohydrate metabolism, whereas endurance training has been shown to improve regulatory control of skeletal muscle carbohydrate metabolism, more so when coupled with obesity. A majority of this literature has focused on PDK, with little information available on PDP. To determine the relative role of PDP in regulating skeletal muscle PDH activity with obesity and endurance training, obese and lean Zucker rats remained sedentary or were endurance trained (1 h/day, 5 days/wk) for a period of 8 wk. Soleus, red gastrocnemius, (RG), and white gastrocnemius (WG) muscles were sampled after the training period. The main findings were 1) obesity resulted in a 46% decrease in PDP activity expressed per milligram extracted mitochondrial protein only in RG, while PDP isoform content was unchanged; 2) 8 wk of endurance training led to a significant 1.4-2.2-fold increase in PDP activity of all muscle examined from obese rats, and the concomitant increase in PDP1 protein was only seen in soleus and RG; 3) 8 wk of endurance training led to a trending 1.4-2.2-fold increase in PDP activity of all muscle examined from obese rats, and the concomitant increase in PDP1 protein was only seen in soleus and RG; and 4) PDP2 protein content was not affected by obesity or training. These results suggest that decreased PDP activity in oxidative skeletal muscles may play a role in the impairment of carbohydrate metabolism in obese rats, which is reversible with endurance training. 相似文献
59.
Richard R. E. Uwiera John P. Kastelic G. Douglas Inglis 《Journal of visualized experiments : JoVE》2009,(28)
The intestine is a complex structure that is involved not only in absorption of nutrients, but also acts as a barrier between the individual and the outside world. As such, the intestine plays a pivotal role in immunosurveillance and protection from enteric pathogens. Investigating intestinal physiology and immunology commonly employs ''intestinal loops'' as an experimental model. The majority of these loop models are non-recovery surgical procedures that study short-term (<24 hr) changes in the intestine (1-3). We previously created a recovery intestinal loop model to specifically measure long-term (<6 mo) immunological changes in the intestine of sheep following exposure to vaccines, adjuvants, and viruses (4). This procedure localized treatments to a specific ''loop'', allowing us to sample this area of the intestine. A significant drawback of this method is the single window of opportunity to administer treatments (i.e. at the time of surgery). Furthermore, samples of both the intestinal mucosa and luminal contents can only be taken at the termination of the project. Other salient limitations of the above model are that the surgical manipulation and requisite post-operative measures (e.g. administration of antibiotics and analgesics) can directly affect the treatment itself and/or alter immune function, thereby confounding results. Therefore, we modified our intestinal loop model by inserting long-term catheters into the loops. Sheep recover fully from the procedure, and are unaffected by the exteriorized catheters. Notably, the establishment of catheters in loops allows us to introduce multiple treatments over an extended interval, following recovery from surgery and clearance of drugs administered during surgery and the post-operative period.Open in a separate windowClick here to view.(66M, flv) 相似文献
60.