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JUDITH H. ROBINS MELANIE HINGSTON ELIZABETH MATISOO‐SMITH HOWARD A. ROSS 《Molecular ecology resources》2007,7(5):717-729
In recent years, research has shown that geographical variation in mitochondrial DNA of commensal rats provides a strong signal of human dispersal and migration. However, interpretation of genetic variation is complicated by the presence of multiple species of Rattus especially in Island Southeast Asia, by the occurrence of some of these Rattus sp. as subfossils in archaeological and natural sites, and by the difficulty of osteological identification of these remains. Amplification of DNA from ancient sources usually yields only small fragments (~200 bp). We assessed whether we could identify Rattus sp. reliably with DNA barcoding using cytochrome oxidase I (COI) sequences, or tree‐based methods using D‐loop, cytochrome b and COI sequences. Species forming well‐differentiated clades in a molecular phylogeny were accurately identified by both methods, even when we used short DNA fragments. Identification was less accurate for paraphyletic and polyphyletic species. We suggest that taxonomic revisions that recognize cryptic or polytypic species will lead to even greater accuracy of DNA‐based identification methods. 相似文献
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Y. CHING CROZIER XIAO CHENG JIA JIN YAN YAO ASHLEY R. FIELD JAMES M. COOK ROSS H. CROZIER 《Molecular ecology resources》2007,7(1):57-59
We developed 11 polymorphic microsatellite loci each for the figs Ficus (Sycomorus) racemosa and Ficus (Urostigma) rubiginosa from AG‐ and TG‐enriched genomic libraries. These 22 loci were investigated for cross‐species amplification and polymorphism in 17–21 F. racemosa and 16–24 F. rubiginosa individuals from Townsville, Australia. Observed heterozygosities range from 0.12 to 0.90 in F. racemosa and from 0.25 to 1.0 in F. rubiginosa. 相似文献
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Nonparametric regression in the presence of measurement error 总被引:4,自引:0,他引:4
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OSKAR FRANKLIN † ROSS E. McMURTRIE COLLEEN M. IVERSEN‡§ KRISTINE Y. CROUS¶ ADRIEN C. FINZI DAVID T. TISSUE †† DAVID S. ELLSWORTH†† RAM OREN‡‡ RICHARD J. NORBY§ 《Global Change Biology》2009,15(1):132-144
Despite the importance of nitrogen (N) limitation of forest carbon (C) sequestration at rising atmospheric CO2 concentration, the mechanisms responsible are not well understood. To elucidate the interactive effects of elevated CO2 (eCO2) and soil N availability on forest productivity and C allocation, we hypothesized that (1) trees maximize fitness by allocating N and C to maximize their net growth and (2) that N uptake is controlled by soil N availability and root exploration for soil N. We tested this model using data collected in Free‐Air CO2 Enrichment sites dominated by evergreen (Pinus taeda; Duke Forest) and deciduous [Liquidambar styraciflua; Oak Ridge National Laboratory (ORNL)] trees. The model explained 80–95% of variation in productivity and N‐uptake data among eCO2, N fertilization and control treatments over 6 years. The model explains why fine‐root production increased, and why N uptake increased despite reduced soil N availability under eCO2 at ORNL and Duke. In agreement with observations at other sites, the model predicts that soil N availability reduced below a critical level diminishes all eCO2 responses. At Duke, a negative feedback between reduced soil N availability and N uptake prevented progressive reduction in soil N availability at eCO2. At ORNL, soil N availability progressively decreased because it did not trigger reductions in N uptake; N uptake was maintained at ORNL through a large increase in the production of fast turnover fine roots. This implies that species with fast root turnover could be more prone to progressive N limitation of carbon sequestration in woody biomass than species with slow root turnover, such as evergreens. However, longer term data are necessary for a thorough evaluation of this hypothesis. The success of the model suggests that the principle of maximization of net growth to control growth and allocation could serve as a basis for simplification and generalization of larger scale forest and ecosystem models, for example by removing the need to specify parameters for relative foliage/stem/root allocation. 相似文献
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