全文获取类型
收费全文 | 2243篇 |
免费 | 321篇 |
出版年
2021年 | 30篇 |
2019年 | 24篇 |
2017年 | 24篇 |
2016年 | 40篇 |
2015年 | 56篇 |
2014年 | 71篇 |
2013年 | 82篇 |
2012年 | 123篇 |
2011年 | 108篇 |
2010年 | 62篇 |
2009年 | 66篇 |
2008年 | 100篇 |
2007年 | 93篇 |
2006年 | 76篇 |
2005年 | 81篇 |
2004年 | 92篇 |
2003年 | 96篇 |
2002年 | 70篇 |
2001年 | 69篇 |
2000年 | 56篇 |
1999年 | 42篇 |
1998年 | 17篇 |
1997年 | 26篇 |
1996年 | 30篇 |
1995年 | 22篇 |
1994年 | 21篇 |
1993年 | 30篇 |
1992年 | 61篇 |
1991年 | 57篇 |
1990年 | 52篇 |
1989年 | 49篇 |
1988年 | 64篇 |
1987年 | 57篇 |
1986年 | 45篇 |
1985年 | 39篇 |
1984年 | 25篇 |
1983年 | 23篇 |
1980年 | 22篇 |
1979年 | 19篇 |
1977年 | 21篇 |
1976年 | 20篇 |
1975年 | 19篇 |
1974年 | 24篇 |
1973年 | 24篇 |
1972年 | 21篇 |
1971年 | 32篇 |
1969年 | 17篇 |
1968年 | 19篇 |
1967年 | 18篇 |
1966年 | 20篇 |
排序方式: 共有2564条查询结果,搜索用时 15 毫秒
141.
Simon I. Hay Katherine E. Battle David M. Pigott David L. Smith Catherine L. Moyes Samir Bhatt John S. Brownstein Nigel Collier Monica F. Myers Dylan B. George Peter W. Gething 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2013,368(1614)
The primary aim of this review was to evaluate the state of knowledge of the geographical distribution of all infectious diseases of clinical significance to humans. A systematic review was conducted to enumerate cartographic progress, with respect to the data available for mapping and the methods currently applied. The results helped define the minimum information requirements for mapping infectious disease occurrence, and a quantitative framework for assessing the mapping opportunities for all infectious diseases. This revealed that of 355 infectious diseases identified, 174 (49%) have a strong rationale for mapping and of these only 7 (4%) had been comprehensively mapped. A variety of ambitions, such as the quantification of the global burden of infectious disease, international biosurveillance, assessing the likelihood of infectious disease outbreaks and exploring the propensity for infectious disease evolution and emergence, are limited by these omissions. An overview of the factors hindering progress in disease cartography is provided. It is argued that rapid improvement in the landscape of infectious diseases mapping can be made by embracing non-conventional data sources, automation of geo-positioning and mapping procedures enabled by machine learning and information technology, respectively, in addition to harnessing labour of the volunteer ‘cognitive surplus’ through crowdsourcing. 相似文献
142.
John L. Mokili Bas E. Dutilh Yan Wei Lim Bradley S. Schneider Travis Taylor Matthew R. Haynes David Metzgar Christopher A. Myers Patrick J. Blair Bahador Nosrat Nathan D. Wolfe Forest Rohwer 《PloS one》2013,8(3)
As part of a virus discovery investigation using a metagenomic approach, a highly divergent novel Human papillomavirus type was identified in pooled convenience nasal/oropharyngeal swab samples collected from patients with febrile respiratory illness. Phylogenetic analysis of the whole genome and the L1 gene reveals that the new HPV identified in this study clusters with previously described gamma papillomaviruses, sharing only 61.1% (whole genome) and 63.1% (L1) sequence identity with its closest relative in the Papillomavirus episteme (PAVE) database. This new virus was named HPV_SD2 pending official classification. The complete genome of HPV-SD2 is 7,299 bp long (36.3% G/C) and contains 7 open reading frames (L2, L1, E6, E7, E1, E2 and E4) and a non-coding long control region (LCR) between L1 and E6. The metagenomic procedures, coupled with the bioinformatic methods described herein are well suited to detect small circular genomes such as those of human papillomaviruses. 相似文献
143.
Kayode K. Ojo Ranae M. Ranade Zhongsheng Zhang David M. Dranow Janette B. Myers Ryan Choi Steve Nakazawa Hewitt Thomas E. Edwards Douglas R. Davies Donald Lorimer Stephen M. Boyle Lynn K. Barrett Frederick S. Buckner Erkang Fan Wesley C. Van Voorhis 《PloS one》2016,11(8)
We investigated Brucella melitensis methionyl-tRNA-synthetase (BmMetRS) with molecular, structural and phenotypic methods to learn if BmMetRS is a promising target for brucellosis drug development. Recombinant BmMetRS was expressed, purified from wild type Brucella melitensis biovar Abortus 2308 strain ATCC/CRP #DD-156 and screened by a thermal melt assay against a focused library of one hundred previously classified methionyl-tRNA-synthetase inhibitors of the blood stage form of Trypanosoma brucei. Three compounds showed appreciable shift of denaturation temperature and were selected for further studies on inhibition of the recombinant enzyme activity and cell viability against wild type B. melitensis strain 16M. BmMetRS protein complexed with these three inhibitors resolved into three-dimensional crystal structures and was analyzed. All three selected methionyl-tRNA-synthetase compounds inhibit recombinant BmMetRS enzymatic functions in an aminoacylation assay at varying concentrations. Furthermore, growth inhibition of B. melitensis strain 16M by the compounds was shown. Inhibitor-BmMetRS crystal structure models were used to illustrate the molecular basis of the enzyme inhibition. Our current data suggests that BmMetRS is a promising target for brucellosis drug development. However, further studies are needed to optimize lead compound potency, efficacy and safety as well as determine the pharmacokinetics, optimal dosage, and duration for effective treatment. 相似文献
144.
Trey K. Sato Mary Tremaine Lucas S. Parreiras Alexander S. Hebert Kevin S. Myers Alan J. Higbee Maria Sardi Sean J. McIlwain Irene M. Ong Rebecca J. Breuer Ragothaman Avanasi Narasimhan Mick A. McGee Quinn Dickinson Alex La Reau Dan Xie Mingyuan Tian Jeff S. Piotrowski Jennifer L. Reed Yaoping Zhang Joshua J. Coon Chris Todd Hittinger Audrey P. Gasch Robert Landick 《PLoS genetics》2016,12(11)
145.
146.
147.
Susan K. Boehlein Peng Liu Ashley Webster Camila Ribeiro Masaharu Suzuki Shan Wu Jiahn‐Chou Guan Jon D. Stewart William F. Tracy A. Mark Settles Donald R. McCarty Karen E. Koch Larkin C. Hannah Tracie A. Hennen‐Bierwagen Alan M. Myers 《The Plant journal : for cell and molecular biology》2019,99(1):23-40
148.
Kelsey Myers Fatima Mohammed Justin W. Rickard Donald E. Meyer 《Journal of applied animal welfare science : JAAWS》2019,22(1):97-104
This paper presents the results of an investigation to determine perceptions, awareness, and knowledge of the unwanted horse population in Illinois from the viewpoint of horse owners, non-horse owners, and equine industry stakeholders. A questionnaire included items that pertained to knowledge of current legislation, equine background, current methods of controlling the unwanted horse population, and methods that respondents believe would reduce the unwanted horse population in Illinois. Results indicated that 58% of horse owners viewed horses as companion animals. Respondents perceived financial hardship to be the major reason why horses become unwanted. Current methods of managing unwanted horse populations were found to be ineffective. Reducing the costs of euthanasia and carcass disposal, allowing processing facilities to reopen in Illinois, and increasing the availability of gelding programs emerged as the most effective ways to manage the unwanted horse population. Results of this survey may lead to greater awareness of the unwanted horse population in Illinois. Furthermore, these results may lead to discussions about future legislation in the State designed to support and manage unwanted horses. 相似文献
149.
150.