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51.
52.
Characterization of a ribonuclease-sensitive nucleoside triphosphatase activity from HeLa nuclei. 下载免费PDF全文
Approximately one-third of the total ATP-hydrolysis activity in isolated HeLa nuclei is sensitive to RNAase (ribonuclease). This activity is selectively extracted with pulse-labelled RNA. In the extracts it co-sediments with various particles with sedimentation coefficients from 10S to 50S, but especially with 24S and 40S particles. ATP hydrolysis by the isolated particles was inhibited extensively (greater than 80%) by RNAase A, heparin and 0.2 M-NaCl. The activity of RNAase-treated particles was recovered when poly(A) was added, but not when DNA was added. The isolated particles exhibited RNAase-sensitive hydrolysis activities for dATP, GTP, CTP and UTP as well as for ATP, and the UTPase activity in the extracts showed nearly the same sedimentation distribution as the ATPase activity. When samples of isolated particles were irradiated with u.v. light in the presence of [alpha-32P]ATP, a 39 kDa polypeptide with a broad distribution from 10S to 50S like that of the ATPase and a 55 kDa polypeptide with a sharp distribution at 24S were photolabelled. Taken together, the data suggest that ATP-hydrolysis activity found in nuclear ribonucleoprotein subfractions appears to be the result of one or two RNA-dependent NTPases that are normally associated with endogenous RNA in a wide variety of particles. 相似文献
53.
Cytogenetic effects of benzene: dosimetric studies on rats exposed to benzene vapour 总被引:1,自引:0,他引:1
Rats were exposed to benzene vapour at nominal concentrations in air of 1, 10, 100 and 1000 ppm acutely for 6 h. Bone marrow cells from each animal were examined for chromosomal abnormalities 24 h after the end of the exposure period. This analysis was carried out on 250 metaphases per animal where possible and showed a significant increase in the percentage of cells with chromosomal abnormalities, excluding gaps, in the groups of animals exposed to 100 and 1000 ppm benzene. In the 10-ppm and 1-ppm exposure groups there were elevated levels of cells with abnormalities which showed evidence of being dose-related, although they were not statistically significant. 相似文献
54.
55.
56.
Assignment of orthologous relationships among mammalian alpha-globin genes by examining flanking regions reveals a rapid rate of evolution 总被引:1,自引:0,他引:1
In order to study the relationships among mammalian alpha-globin genes, we
have determined the sequence of the 3' flanking region of the human alpha 1
globin gene and have made pairwise comparisons between sequenced
alpha-globin genes. The flanking regions were examined in detail because
sequence matches in these regions could be interpreted with the least
complication from the gene duplications and conversions that have occurred
frequently in mammalian alpha-like globin gene clusters. We found good
matches between the flanking regions of human alpha 1 and rabbit alpha 1,
human psi alpha 1 and goat I alpha, human alpha 2 and goat II alpha, and
horse alpha 1 and goat II alpha. These matches were used to align the
alpha-globin genes in gene clusters from different mammals. This alignment
shows that genes at equivalent positions in the gene clusters of different
mammals can be functional or nonfunctional, depending on whether they
corrected against a functional alpha-globin gene in recent evolutionary
history. The number of alpha-globin genes (including pseudogenes) appears
to differ among species, although highly divergent pseudogenes may not have
been detected in all species examined. Although matching sequences could be
found in interspecies comparisons of the flanking regions of alpha- globin
genes, these matches are not as extensive as those found in the flanking
regions of mammalian beta-like globin genes. This observation suggests that
the noncoding sequences in the mammalian alpha-globin gene clusters are
evolving at a faster rate than those in the beta-like globin gene clusters.
The proposed faster rate of evolution fits with the poor conservation of
the genetic linkage map around alpha-globin gene clusters when compared to
that of the beta-like globin gene clusters. Analysis of the 3' flanking
regions of alpha-globin genes has revealed a conserved sequence
approximately 100-150 bp 3' to the polyadenylation site; this sequence may
be involved in the expression or regulation of alpha-globin genes.
相似文献
57.
58.
U. Ingrid Richardson 《Life sciences》1983,33(20):1981-1988
Synthetic corticotropin-releasing factor (CRF) stimulates ACTH secretion in the clonal mouse pituitary cell strain AtT20/D16v (D16) in a dose-dependent manner with a half-maximal effect at 2×10?9M. A single dose of 5×10?9M CRF maximally stimulates the rate of ACTH secretion during the initial two hrs of treatment. During the period of maximal CRF stimulation intracellular hormone concentration declines progressively to a nadir at 4 hrs. During the ensuing 24 hrs of incubation intracellular hormone levels in CRF-stimulated cells increase gradually toward control values. Somatostatin (SRIF) inhibits the secretory response to CRF. This action of SRIF is dose-dependent with a half-maximal effect at 1×10?9M and results in decreased maximal ACTH secretion with little effect on the ED50 for CRF. 相似文献
59.
60.
Migration over Puerto Rico was recorded by time-lapse filming of the display of a long-range surveillance radar on 40 days and 37 nights in the period 2 March-29 May 1971. Moderate density movements occurred every night; low density movements occurred on most days. Many birds, primarily passerines, took off from Puerto Rico each evening at 20–45 minutes after sunset.
Almost all birds flew to the west, NW or north. Birds were seen approaching from the direction of the Windward Islands and Venezuela, over Puerto Rico, and departing towards the Bahamas and eastern coast of the U.S. Uni- and multivariate analyses showed that the number of birds departing W-N each evening was positively correlated with following winds.
There is less night-to-night variation in the amount of migration at Puerto Rico than in eastern North America. However, this is apparently the result of less variable weather in the tropics, not the result of any lesser degree of meteorological selectivity by the migrants.
The tracks of the birds were correlated with wind direction. Birds moved WNW-NW with NE side winds but NW-NNW with SE following winds. The tracks were rarely exactly downwind. The variance amongst the directions of individual birds at any given time was usually small and not correlated with cloud cover or magnetic disturbances. The estimated headings of the birds varied from day to day in a pattern suggesting adjustment of headings to compensate at least partially for lateral wind drift.
In autumn many birds approach Puerto Rico from the north or even east of north; in spring few birds moved in the opposite directions. This difference in routes takes advantage of prevailing wind patterns. 相似文献
Almost all birds flew to the west, NW or north. Birds were seen approaching from the direction of the Windward Islands and Venezuela, over Puerto Rico, and departing towards the Bahamas and eastern coast of the U.S. Uni- and multivariate analyses showed that the number of birds departing W-N each evening was positively correlated with following winds.
There is less night-to-night variation in the amount of migration at Puerto Rico than in eastern North America. However, this is apparently the result of less variable weather in the tropics, not the result of any lesser degree of meteorological selectivity by the migrants.
The tracks of the birds were correlated with wind direction. Birds moved WNW-NW with NE side winds but NW-NNW with SE following winds. The tracks were rarely exactly downwind. The variance amongst the directions of individual birds at any given time was usually small and not correlated with cloud cover or magnetic disturbances. The estimated headings of the birds varied from day to day in a pattern suggesting adjustment of headings to compensate at least partially for lateral wind drift.
In autumn many birds approach Puerto Rico from the north or even east of north; in spring few birds moved in the opposite directions. This difference in routes takes advantage of prevailing wind patterns. 相似文献