全文获取类型
收费全文 | 3145篇 |
免费 | 384篇 |
国内免费 | 5篇 |
出版年
2021年 | 37篇 |
2019年 | 34篇 |
2018年 | 36篇 |
2017年 | 43篇 |
2016年 | 44篇 |
2015年 | 97篇 |
2014年 | 127篇 |
2013年 | 123篇 |
2012年 | 154篇 |
2011年 | 165篇 |
2010年 | 100篇 |
2009年 | 78篇 |
2008年 | 131篇 |
2007年 | 129篇 |
2006年 | 130篇 |
2005年 | 119篇 |
2004年 | 117篇 |
2003年 | 115篇 |
2002年 | 124篇 |
2001年 | 76篇 |
2000年 | 87篇 |
1999年 | 75篇 |
1998年 | 48篇 |
1997年 | 25篇 |
1996年 | 35篇 |
1995年 | 36篇 |
1994年 | 34篇 |
1992年 | 68篇 |
1991年 | 57篇 |
1990年 | 59篇 |
1989年 | 46篇 |
1988年 | 50篇 |
1987年 | 56篇 |
1986年 | 47篇 |
1985年 | 57篇 |
1984年 | 49篇 |
1983年 | 39篇 |
1982年 | 35篇 |
1981年 | 29篇 |
1980年 | 34篇 |
1979年 | 49篇 |
1978年 | 26篇 |
1977年 | 41篇 |
1976年 | 28篇 |
1975年 | 29篇 |
1974年 | 35篇 |
1973年 | 34篇 |
1972年 | 25篇 |
1968年 | 25篇 |
1967年 | 31篇 |
排序方式: 共有3534条查询结果,搜索用时 156 毫秒
191.
192.
193.
194.
Mapping soybean aphid resistance genes in PI 567598B 总被引:1,自引:0,他引:1
Carmille Bales Guorong Zhang Menghan Liu Clarice Mensah Cuihua Gu Qijian Song David Hyten Perry Cregan Dechun Wang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(8):2081-2091
The soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America since it was first reported in 2000. Our previous study revealed that the strong aphid resistance of plant introduction (PI) 567598B was controlled by two recessive genes. The objective of this study was to locate these two genes on the soybean genetic linkage map using molecular markers. A mapping population of 282 F4:5 lines derived from IA2070 × E06902 was evaluated for aphid resistance in a field trial in 2009 and a greenhouse trial in 2010. Two quantitative trait loci (QTLs) were identified using the composite and multiple interval mapping methods, and were mapped on chromosomes 7 (linkage group M) and 16 (linkage group J), respectively. E06902, a parent derived from PI 567598B, conferred resistance at both loci. In the 2010 greenhouse trial, each of the two QTLs explained over 30 % of the phenotypic variation. Significant epistatic interaction was also found between these two QTLs. However, in the 2009 field trial, only the QTL on chromosome 16 was found and it explained 56.1 % of the phenotypic variation. These two QTLs and their interaction were confirmed with another population consisting of 94 F2:5 lines in the 2008 and 2009 greenhouse trials. For both trials in the alternative population, these two loci explained about 50 and 80.4 % of the total phenotypic variation, respectively. Our study shows that soybean aphid isolate used in the 2009 field trial defeated the QTL found on chromosome 7. Presence of the QTL on chromosome 16 conferred soybean aphid resistance in all trials. The markers linked to the aphid-resistant QTLs in PI 567598B or its derived lines can be used in marker-assisted breeding for aphid resistance. 相似文献
195.
Daria?S. Khvostichenko Johnathan?J.D. Ng Sarah?L. Perry Monisha Menon Paul?J.A. Kenis 《Biophysical journal》2013,105(8):1848-1859
Using small-angle x-ray scattering (SAXS), we investigated the phase behavior of mesophases of monoolein (MO) mixed with additives commonly used for the crystallization of membrane proteins from lipidic mesophases. In particular, we examined the effect of sodium and potassium phosphate salts and the detergent β-octylglucoside (βOG) over a wide range of compositions relevant for the crystallization of membrane proteins in lipidic mesophases. We studied two types of systems: 1), ternary mixtures of MO with salt solutions above the hydration boundary; and 2), quaternary mixtures of MO with βOG and salt solutions over a wide range of hydration conditions. All quaternary mixtures showed highly regular lyotropic phase behavior with the same sequence of phases (Lα, Ia3d, and Pn3m) as MO/water mixtures at similar temperatures. The effects of additives in quaternary systems agreed qualitatively with those found in ternary mixtures in which only one additive is present. However, quantitative differences in the effects of additives on the lattice parameters of fully hydrated mesophases were found between ternary and quaternary mixtures. We discuss the implications of these findings for mechanistic investigations of membrane protein crystallization in lipidic mesophases and for studies of the suitability of precipitants for mesophase-based crystallization methods. 相似文献
196.
Karin Chen Emily?M. Coonrod Attila Kumánovics Zechariah F. Franks Jacob?D. Durtschi Rebecca?L. Margraf Wilfred Wu Nahla?M. Heikal Nancy?H. Augustine Perry?G. Ridge Harry?R. Hill Lynn?B. Jorde Andrew?S. Weyrich Guy?A. Zimmerman Adi?V. Gundlapalli John?F. Bohnsack Karl?V. Voelkerding 《American journal of human genetics》2013,93(5):812-824
Common variable immunodeficiency (CVID) is a heterogeneous disorder characterized by antibody deficiency, poor humoral response to antigens, and recurrent infections. To investigate the molecular cause of CVID, we carried out exome sequence analysis of a family diagnosed with CVID and identified a heterozygous frameshift mutation, c.2564delA (p.Lys855Serfs∗7), in NFKB2 affecting the C terminus of NF-κB2 (also known as p100/p52 or p100/p49). Subsequent screening of NFKB2 in 33 unrelated CVID-affected individuals uncovered a second heterozygous nonsense mutation, c.2557C>T (p.Arg853∗), in one simplex case. Affected individuals in both families presented with an unusual combination of childhood-onset hypogammaglobulinemia with recurrent infections, autoimmune features, and adrenal insufficiency. NF-κB2 is the principal protein involved in the noncanonical NF-κB pathway, is evolutionarily conserved, and functions in peripheral lymphoid organ development, B cell development, and antibody production. In addition, Nfkb2 mouse models demonstrate a CVID-like phenotype with hypogammaglobulinemia and poor humoral response to antigens. Immunoblot analysis and immunofluorescence microscopy of transformed B cells from affected individuals show that the NFKB2 mutations affect phosphorylation and proteasomal processing of p100 and, ultimately, p52 nuclear translocation. These findings describe germline mutations in NFKB2 and establish the noncanonical NF-κB signaling pathway as a genetic etiology for this primary immunodeficiency syndrome. 相似文献
197.
198.
199.
Jeffrey A. Deiuliis Rafay Syed Dheeraj Duggineni Jessica Rutsky Palanivel Rengasamy Jie Zhang Kun Huang Bradley Needleman Dean Mikami Kyle Perry Jeffrey Hazey Sanjay Rajagopalan 《PloS one》2016,11(11)
Obesity in humans and mice is typified by an activated macrophage phenotype in the visceral adipose tissue (VAT) leading to increased macrophage-mediated inflammation. microRNAs (miRNAs) play an important role in regulating inflammatory pathways in macrophages, and in this study we compared miRNA expression in the VAT of insulin resistant morbidly obese humans to a non-obese cohort with normal glucose tolerance. miR-223-3p was found to be significantly upregulated in the whole omental tissue RNA of 12 human subjects, as were 8 additional miRNAs. We then confirmed that miR-223 upregulation was specific to the stromal vascular cells of human VAT, and found that miR-223 levels were unchanged in adipocytes and circulating monocytes of the non-obese and obese. miR-223 ablation increased basal / unstimulated TLR4 and STAT3 expression and LPS-stimulated TLR4, STAT3, and NOS2 expression in primary macrophages. Conversely, miR-223 mimics decreased TLR4 expression in primary macrophage, at the same time it negatively regulated FBXW7 expression, a well described suppressor of Toll-like receptor 4 (TLR4) signaling. We concluded that the abundance of miR-223 in macrophages significantly modulates macrophage phenotype / activation state and response to stimuli via effects on the TLR4/FBXW7 axis. 相似文献
200.