快速测定厚朴中厚朴酚及其含量的方法

目的:通过建立系统的HPLC快速检测方法,分析厚朴中主要药用成分厚朴酚与和厚朴酚的含量,确定厚朴的药用价值及开发利用价值.方法:用HPLC法对张家界、隆回及恩施地区的厚朴植株的不同部位的药材中厚朴酚与和厚朴酚的含量进行了含量的分析.结果:结果快速、准确、重现性好、HPLC分析图谱理想.结论:本文所运用的HPLC分析条件能快速准确的鉴定厚朴酚及和厚朴酚的含量,确定厚朴药材的品质,并对混乱的药材市场有一定的监控作用.     

Variability and divergence in Pongamia pinnata (L.) Pierre germplasm – a candidate tree for biodiesel

Three explorations were undertaken in South East Coastal zone of India covering parts of Andhra Pradesh (AP) and Orissa states to collect Pongamia pinnata (L.) Pierre germplasm during March–June 2007. A total of 123 accessions were collected and seed data recorded were analyzed for morphometric traits viz., seed length, seed width, seed thickness, 100‐seed weight and oil content. Variation in the collected germplasm was analyzed using anova , simple measures of variation and D² statistics. Significant genetic variability between seed traits and oil content and association among the seed traits was recorded. Phenotypic variance was higher than genotypic variance for all the characters indicating dominant role of environment. High heritability (broad sense) for 100‐seed weight (97.6%) and oil content (86.7%) indicated the reliability of these characters as selection criteria for plus trees. Genetic gain was maximum for 100‐seed weight (62.6%) followed by oil content (30.5%). D² analysis grouped the accessions into 12 clusters. Cluster XII and cluster IX were the most diverse based on the intercluster distance. Based on the observed diversity, Chittoor, Srikakulam and Adilabad districts of AP are most suitable for collecting diverse germplasm lines and also for in situ conservation.     

ORF66 protein kinase function is required for T-cell tropism of varicella-zoster virus in vivo

Several functions have been attributed to the serine/threonine protein kinase encoded by open reading frame 66 (ORF66) of varicella-zoster virus (VZV), including modulation of the apoptosis and interferon pathways, down-regulation of major histocompatibility complex class I cell surface expression, and regulation of IE62 localization. The amino acid sequence of the ORF66 protein contains a recognizable conserved kinase domain. Point mutations were introduced into conserved protein kinase motifs to evaluate their importance to ORF66 protein functions. Two substitution mutants were generated, including a G102A substitution, which blocked autophosphorylation and altered IE62 localization, and an S250P substitution, which had no effect on either autophosphorylation or IE62 localization. Both kinase domain mutants grew to titers equivalent to recombinant parent Oka (pOka) in vitro. pOka66G102A had slightly reduced growth in skin, which was comparable to the reduction observed when ORF66 translation was prevented by stop codon insertions in pOka66S. In contrast, infection of T-cell xenografts with pOka66G102A was associated with a significant decrease in infectious virus production equivalent to the impaired T-cell tropism found with pOka66S infection of T-cell xenografts in vivo. Disrupting kinase activity with the G102A mutation did not alter IE62 cytoplasmic localization in VZV-infected T cells, suggesting that decreased T-cell tropism is due to other ORF66 protein functions. The G102A mutation reduced the antiapoptotic effects of VZV infection of T cells. These experiments indicate that the T-cell tropism of VZV depends upon intact ORF66 protein kinase function.     

云前胡的萜类成分研究

从云前胡(Peucedanum rubricauleSheh et Shan)的乙醇提取物中首次分离并鉴定了4个萜类化合物,分别为sinodielides A(1)、B(2),积雪草酸(asiatic acid,3)和乌苏酸(ursolic acid,4)。     

广东省爬行类新纪录——海南棱蜥

2010年6月2日,在广东省韶关市始兴县车八岭国家级自然保护区采集到蜥蜴幼体标本1号,经鉴定为海南棱蜥(Tropidophorus hainanus),为广东省爬行动物新纪录,标本保存在华南濒危动物研究所。     

基于树干液流测定值进行尺度扩展的马占相思林段蒸腾和冠层气孔导度

应用Granier热消散探针,长期监测华南丘陵地马占相思(Acacia mangium)林14棵样树的树干液流(Sap flow),由此计算整树和林段的蒸腾速率,结合同步记录的环境因子,求算冠层平均气孔导度(G_c)。Granier探针的灵敏度较高,能精确测定即使是微弱的液流活动。观测结果显示,树木个体之间的液流密度(J_s)和整树蒸腾(E_t)受树形特征影响较大。马占相思林径级大的树木个体数较少,但占据林段边材总面积和林段蒸腾的比例较大。J_s和E_t的日变化主要受光合有效辐射(Q_o)和空气水蒸气压亏缺(D)的控制,土壤含水量(θ)对较大胸径树木E_t的影响大于胸径较小的树木,个体之间J_s和E_t的差异随θ的下降而缩小。一年中,林段蒸腾(E)在光照和水热条件较好的7月最高,9~12月,由于土壤水分供应的减少致使E值降低,E对D的敏感性下降。G_c与主要环境因子的关系与E相似,如果θ长期偏低,G_c会明显下降,是造成E降低的主要原因。成熟马占相思林在光照充足、水热条件较好的情况下的蒸腾活动旺盛,但对土壤水分胁迫的忍受力较低。     

基于耦合双向聚类技术的DLBCL异质性分析

基因芯片技术为疾病异质性研究提供了有力的工具。当前基于传统聚类分析的方法一般利用芯片上大量基因作为特征来发现疾病的亚型, 因此它们没有考虑到特征中包含的大量无关基因会掩盖有意义的疾病样本的分割。为了避免这个缺点, 提出了基于耦合双向聚类的异质性分析方法(Heterogeneous Analysis Based on Coupled Two-Way Clustering, HCTWC)来搜索有意义的基因簇以便发现样本的内在分割。该方法被应用于弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma DLBCL)芯片数据集, 通过识别的基因簇作为特征对DLBCL样本聚类发现生存期分别为55%和25%的两类DLBCL亚型(P<0.05), 因此, HCTWC方法在解决疾病异质性是有效的。     

广东车八岭自然保护区蕨类植物资源及其综合利用

据调查统计,车八岭国家级自然保护区共有蕨类植物38科80属195种。根据经济价值、应用和生长状况,将其划分为7类,分别是药用类、食用类、观赏类、工业用类、指示类和珍稀特有类;本文还就该保护区蕨类植物资源的现状与特点,提出合理的开发利用建议。     

中国淡水蛏精子发生的超微结构研究

利用透射电镜观察了中国淡水蛏的精子发生和精子结构。描述了精子发生过程中各细胞形态结构的变化;揭示了细胞核、线粒体和项体等的演变过程。中国淡水蛏的精子经过精原细胞、初级精母细胞、次级精母细胞和精子细胞,最终限育为成熟的精子。精子为典型的原生型,由头部、中段和尾部构成。项体复合体位于精子头部前端,呈“⊥”形,精核近圆形;中段包括２个中心粒和４或５个紧密排列的椭圆形线粒体;尾部鞭毛断面为典型的“９＋２”