Phase-related 6-kDa peptide titre in haemolymph of larvae and adult Schistocerca gregaria and its role in yellow-protein synthesis

Abstract.  The haemolymph titre of the phase-related 6-kDa peptide is determined in fourth- and fifth-instar larvae, as well as in adults, by high-performance liquid chromatography. In larvae, the concentration of this peptide slowly increases towards the end of the instar to reach a maximum just before the moult. The titre is slightly higher in the fifth instar than in the fourth instar. In adults, it reaches an average concentration of 0.8 μg μL⁻¹ of the haemolymph. In females, a peak is found at day 15 whereas, in males, there are two peaks, the first at day 9 and the second at day 15 after fledging. The role of the peptide in inducing yellow protein mRNA synthesis is investigated, as is its effect on the production of the pheromonal compound phenylacetonitrile (PAN). The peptide stimulates yellow protein mRNA synthesis, but its injection causes no change in yellow colouration. No effect on PAN production is found.