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911.
Richard R. Hudson 《Journal of evolutionary biology》1994,7(5):535-548
Expressions are obtained for the expected levels of linkage disequilibrium under three different equilibrium neutral models that make different assumptions about how recombination takes place. A transformation model is considered in which exchange events involve only one locus at a time. Two conjugation models are considered one with a linear genome and one with a circular genome. In the conjugation models large blocks of genes can be transferred with each conjugation. Consistent with published simulation results, it is found that if the transformation rate per locus is more than twenty times the mutation rate per locus, then the levels of linkage disequilibrium are quite low. If the number of loci being sampled is greater than 10, conjugation with a circular genome can be considerably more effective than transformation in reducing linkage disequilibrium. When recombination rates are high, expected linkage disequilibrium is shown to be proportional to the inverse of the transformation rate (or conjugation rate.) 相似文献
912.
Microbial Hydrocarbon Co-oxidation. III. Isolation and Characterization of an alpha, alpha'-Dimethyl-cis, cis-Muconic Acid-producing Strain of Nocardia corallina
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A soil isolate identified as a strain of Nocardia corallina accumulated α, α′-dimethyl-cis, cis-muconic acid under co-oxidation conditions employing n-hexadecane for growth and p-xylene as the co-oxidizable substrate. N. corallina V-49 was postulated to have two pathways for the oxidation of p-xylene. One pathway proceeds throughp-benzyl alcohol, p-tolualdehyde, and p-toluic acid to 2, 3-dihydroxy-p-toluic acid, and the other pathway results in ortho ring cleavage of 3, 6-dimethylpyrocatechol and hence accumulation of α,α′-dimethyl-cis, cis-muconic acid. 相似文献
913.
914.
Microbial Hydrocarbon Co-oxidation: I. Oxidation of Mono- and Dicyclic Hydrocarbons by Soil Isolates of the Genus Nocardia 总被引:23,自引:15,他引:8
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Nocardia cultures, isolated from soil by use of n-paraffins as the sole carbon source, have been shown to bring about significant oxidation of several methyl-substituted mono- and dicyclic aromatic hydrocarbons. Oxygen uptake by washed cell suspensions was not a reliable indicator of oxidation. Under co-oxidation conditions in shaken flasks, o- and p-xylenes were oxidized to their respective mono-aromatic acids, o-toluic and p-toluic acids. In addition, a new fermentation product, 2, 3-dihydroxy-p-toluic acid, was found in the p-xylene oxidation system. Of 10 methyl-substituted naphthalenes tested (1-methyl, 2-methyl, 1, 3-dimethyl, 1, 4-dimethyl, 1, 5-dimethyl, 1, 8-dimethyl, 1, 6-dimethyl, 2, 3-dimethyl, 2, 6-dimethyl, 2, 7-dimethyl), only those containing a methyl group in the β position were oxidized at this position to the mono acid. 相似文献
915.
An acute effect of antigens on the nuclear histones of mouse thymocytes was investigated by means of cytophotometric measurements of thymocytes stained with ammoniacal-silver (A-S) and with fast green (FG). In addition, the DNA content was measured in terms of Feulgen staining. In terms of such staining it appeared that nuclei of control thymocytes contain a greater amount of nuclear histones and a higher histone/DNA ratio than do renal cell nuclei from the same animal. Within 1 hour after the injection of antigen the thymocyte nuclei appear to lose approximately 32 per cent and 20 per cent, respectively, of A-S and FG stainable nuclear proteins, while the Feulgen staining remains unchanged. Since the renal cell nuclei show no antigen-induced change in histone staining, the histone staining and histone/DNA ratios were found to be similar in the thymocytes and renal cells of the antigen-injected mice. The antigen-induced loss of thymocyte histones was also found to be associated with a change in the color of the A-S staining, from yellowish brown to black. This and other findings suggest that thymocyte nuclei contain an antigen-labile, lysine-rich histone. The implication of these observations in regard to the phenomenon of immunological competence is discussed and the need for continued investigation indicated. 相似文献
916.
David T. Burns Sandra Donkervoort Juliane S. Müller Ellen Knierim Diana Bharucha-Goebel Eissa Ali Faqeih Stephanie K. Bell Abdullah Y. AlFaifi Dorota Monies Francisca Millan Kyle Retterer Sarah Dyack Sara MacKay Susanne Morales-Gonzalez Michele Giunta Benjamin Munro Gavin Hudson Mena Scavina Carsten G. Bönnemann 《American journal of human genetics》2018,102(5):858-873
917.
Sivakumar Swaminathan Nilwala S. Abeysekara Joshua M. Knight Min Liu Jia Dong Matthew E. Hudson Madan K. Bhattacharyya Silvia R. Cianzio 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(5):1047-1062
Key message
Novel QTL conferring resistance to both the SDS and SCN was detected in two RIL populations. Dual resistant RILs could be used in breeding programs for developing resistant soybean cultivars.Abstract
Soybean cultivars, susceptible to the fungus Fusarium virguliforme, which causes sudden death syndrome (SDS), and to the soybean cyst nematode (SCN) (Heterodera glycines), suffer yield losses valued over a billion dollars annually. Both pathogens may occur in the same production fields. Planting of cultivars genetically resistant to both pathogens is considered one of the most effective means to control the two pathogens. The objective of the study was to map quantitative trait loci (QTL) underlying SDS and SCN resistances. Two recombinant inbred line (RIL) populations were developed by crossing ‘A95-684043’, a high-yielding maturity group (MG) II line resistant to SCN, with ‘LS94-3207’ and ‘LS98-0582’ of MG IV, resistant to both F. virguliforme and SCN. Two hundred F7 derived recombinant inbred lines from each population AX19286 (A95-684043 × LS94-3207) and AX19287 (A95-684043 × LS98-0582) were screened for resistance to each pathogen under greenhouse conditions. Five hundred and eighty and 371 SNP markers were used for mapping resistance QTL in each population. In AX19286, one novel SCN resistance QTL was mapped to chromosome 8. In AX19287, one novel SDS resistance QTL was mapped to chromosome 17 and one novel SCN resistance QTL was mapped to chromosome 11. Previously identified additional SDS and SCN resistance QTL were also detected in the study. Lines possessing superior resistance to both pathogens were also identified and could be used as germplasm sources for breeding SDS- and SCN-resistant soybean cultivars.918.
Training-induced skeletal muscle adaptations are independent of systemic adaptations 总被引:1,自引:0,他引:1
J R Minotti E C Johnson T L Hudson G Zuroske E Fukushima G Murata L E Wise T W Chick M V Icenogle 《Journal of applied physiology》1990,68(1):289-294
To isolate the peripheral adaptations to training, five normal subjects exercised the nondominant (ND) wrist flexors for 41 +/- 11 days, maintaining an exercise intensity below the threshold required for cardiovascular adaptations. Before and after training, intracellular pH and the ratio of inorganic phosphate to phosphocreatine (Pi/PCr) were measured by 31P magnetic resonance spectroscopy. Also maximal O2 consumption (VO2 max), muscle mass, and forearm blood flow were determined by graded systemic exercise, magnetic resonance imaging, and venous occlusion plethysmography, respectively. Blood flow, Pi/PCr, and pH were measured in both forearms at rest and during submaximal wrist flexion at 5, 23, and 46 J/min. Training did not affect VO2 max, exercise blood flow, or muscle mass. Resting pH, Pi/PCr, and blood flow were also unchanged. After training, the ND forearm demonstrated significantly lower Pi/PCr at 23 and 46 J/min. Endurance, measured as the number of contractions to exhaustion, also was increased significantly (63%) after training in the ND forearm. We conclude that 1) forearm training results in a lower Pi/PCr at identical submaximal work loads; 2) this improvement is independent of changes in VO2 max, muscle mass, or limb blood flow; and 3) these differences are associated with improved endurance and may reflect improved oxidative capacity of skeletal muscle. 相似文献
919.
920.
Intoxication of Vero cells by ricin, modeccin, diphtheria toxin (DT), and Pseudomonas exotoxin A requires: 1) binding to cell surface receptors; 2) transport to the cytoplasm; and 3) enzymatic inactivation of a component of the protein synthetic machinery. The kinetic profiles of all four toxins consist of a lag followed by the apparent first-order decrease in protein synthesis. Autoradiographic analysis of DT-intoxicated cell populations has demonstrated that two subpopulations of cells exist during the period of decreasing protein synthesis: one population synthesizing at control levels and the other synthesizing little or no protein (Hudson, T. H., and Neville, D. M., Jr. (1985) J. Biol. Chem. 260, 2675-2680). The present study correlates the autoradiographic data with the rates of protein synthesis decline in cells intoxicated with modeccin, ricin, Pseudomonas exotoxin A, as well DT. In all cases, the first time point which exhibits a decrease in protein synthetic activity also exhibits two subpopulations of cells, one synthesizing protein at control rates and the other synthesizing little or no protein. As the intoxication progresses, cells leave the control population by the rapid cessation of all protein synthesis. These experiments demonstrate that transport of all four toxins to the cytosol is the rate-limiting step during the pseudo first-order decline in protein synthesis. Furthermore, the final step in the transport process (translocation) must result in the release to the cytoplasm of a quantity of toxin sufficient to rapidly inactivate all protein synthesis in that cell. The probability of a translocation event occurring in any cell of the population is established during the lag and remains constant throughout the first-order decrease in protein synthesis. The requirement for acidification during the intoxication by DT, Pseudomonas exotoxin A, or modeccin is restricted to the lag period. Acidification is therefore necessary to establish the probability of translocation, but it is not directly involved in the actual translocation of these toxins. The pseudo first-order passage of DT intoxications through antitoxin and NH4Cl- or monensin-sensitive stages are shown to have the same cellular basis as the pseudo first-order decrease in protein synthesis. A kinetic model is presented which defines the DT intoxication process from one of its earliest events (endocytosis) to its penultimate event (translocation of toxin to the cytosol).(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献