蓝斑核对上呼吸道阻力肌—颏舌肌功能的影响

本实验在３８只经氨基甲酸乙酯麻醉的健康家兔上进行,观察了电、化学刺激蓝斑核（ＬＣ）对颏舌肌功能的影响。结果如下：（１）长串电脉冲刺激蓝斑核使颏舌肌肌电活动明显增强,表现为长吸性肌电积分幅度升高,膈肌亦出现与颏舌肌同步的肌电活动增强。（２）ＬＣ内微量注射胞体兴奋剂谷氨酸钠,也引起明显的颏舌肌和膈肌肌电活动增强。（３）上述电、化学刺激的区域对照和盐水对照实验,均未出现有意义的肌电改变。提示：电、化学刺激ＬＣ可特异性增强上呼吸道阻力肌──颏舌肌的紧张性活动,具有减小上呼吸道阻力的作用,这对于某些上呼吸道阻塞性疾病发病机制的研究可能具有重要的意义。     

颧颞部骨折术后并发颞部凹陷的临床回顾性研究

目的：分析不同颧颞部骨折性质与颞部凹陷的相关性,评价颧颞部骨折术后并发颞部凹陷的防治效果。方法：对105例颧颞部骨折病例进行回顾性分析,52例患者行颞部凹陷修复术,采用头皮冠状切口,应用钛网修复颞部凹陷,术后通过长期随访评价治疗效果。结果：陈旧性骨折颞部凹陷的发生率显著高于新鲜骨折,但治疗后颞部外形均有明显改善。结论：钛网植入能有效地修复颧颞部骨折术后并发的颞部凹陷,但应把握手术时机及治疗方法。     

铁蛋白介导的地衣多糖酶胞内自发聚集及其高效制备

酶分离纯化、固定化及催化性能提升一直是生物催化领域的研究热点和前沿,也是众多研究者致力解决的难点.研究和开发新型的纯化、固定化及提升催化性能的方法,降低纯化及储存等设备的要求及生产成本,对酶大规模应用具有重要意义.文中将铁蛋白(ferritin)与目标酶(地衣多糖酶)基因融合,经高效表达后,在细胞内 自发形成无载体固定...     

CPPU提高柚树苗抗旱性的研究

水分胁迫下,柚树苗叶片RWC、ψW、Pn及可溶性蛋白质、叶绿素含量下降,游离脯氨酸、MDA含量及SOD活性升高,CAT活性先上升后下降,APX活性、AsA和GSH含量明显下降,抗旱性强的品种具有较高的活性氧清除能力。CPPU处理提高柚树苗叶片RWC、Pn、叶绿素、游离脯氨酸、AsA、GSH含量,并增强SOD、APX活性,降低MDA水平,从而提高柚树苗抗旱性。实验结果表明,CPPU处理提高叶片对膜脂过氧化作用的保护能力可能是其提高柚树苗抗旱性的原因。     

蕙兰根内可培养细菌的物种多样性

以MS基本培养基添加蕙兰菌根浸出液制成的培养基进行分离培养的方法,从野生蕙兰（Cymbidium faberi）根部首次分离到内生细菌。经过分离纯化培养获得纯菌株27株。经过16S rDNA基因序列测序,并与GenBank数据比对,其相似性均在98%以上,分析鉴定结果表明,存活的22株菌可分为8属14种。分别隶属于伯克氏菌属（Burkholderia）、假单胞菌属（Pseudomonas）、芽胞杆菌属（Bacillus）、Leifsonia属、贪食菌属（Variovorax）、欧文氏菌属（Erwinia）、Duganella属和不动杆菌属（Acinetobacter）。对这些菌株进行分离培养及鉴定有助于理解兰花与微生物之间的相互作用关系,为开发利用这些微生物开辟新的思路。     

人血管内皮细胞中腺苷代谢的定量研究

目的:通过对人脐静脉内皮细胞腺苷分泌进行定性及定量研究,了解人类血管内皮细胞的腺苷代谢及机制.方法:收集并测定不同干预下细胞柱流出液中分离的人脐静脉内皮细胞分泌的腺苷量.结果:在无干预、抑制腺苷激酶及去氨酶、抑制细胞膜腺苷转运情况下,人脐静脉内皮细胞腺苷分泌率分别为13.5±7.1 pmol·min-1·mg-1、32.5±14.2 pmol·min-1·mg-1和20.8±15.7 pmol·min-1·mg-1.结论:人类血管内皮细胞内腺苷合成高于胞外,而细胞膜腺苷转运被抑制后的腺苷分泌率反而高于生理状态下分泌率,则表明腺苷在胞内分解代谢非常迅速,使部分腺苷反由胞外扩散入胞内.     

Nonlocal Immunized Mid-Infrared Magnetic Hot Spots in Graphene Junctions

Magnetic hot spots, which implies confinements and enhancements of magnetic fields, are demonstrated in graphene junctions (GJs) in the mid-infrared range. The appearance of magnetic hot spots in GJs comes from the conduction currents in the junction. In further, the extinction resonance peaks suffer blue shift, along with the increases in the magnetic fields inside junction area, when the junction width reduces. In opposite to the circumstances for electric field enhancements, neither magnetic field enhancements nor resonance frequency of GJs is perturbed by the intrinsic nonlocal electronic response of graphene. Such nonlocality immunized magnetic enhancement could be explained by the polarization dependent property of nonlocal effect.     

Combination of bone tissue engineering and BMP-2 gene transfection promotes bone healing in osteoporotic rats

OBJECTIVE: The aim of this study was to develop a feasible approach to promote bone healing in osteoporotic rats using autogenous bone tissue-engineering and gene transfection of human bone morphogenetic protein 2 (hBMP-2). METHODS: Bone marrow stromal cells (BMSCs) from the left tibia of osteoporotic rats were transfected with the hBMP-2 gene in vitro which was confirmed by immunohistochemistry, in situ hybridization and Western blotting. Autogenous transfected or untransfected BMSCs were seeded on macroporous coral hydroxyapatite (CHA) scaffolds. Each cell-scaffold construct was implanted into a defect site which was created in the ramus of the mandible of osteoporotic rats. Four or eight weeks after implantation in situ hybridization was performed in BMSCs transfected with hBMP-2, X-ray examinations, histological and histomorphological analyses were used to evaluate the effect of tissue-engineered bone on osseous defect repair. RESULTS: Newly formed bone was observed at the margin of the defect 4 weeks after implantation with BMSCs transfected with BMP-2. Mature bone was observed 8 weeks after treatment. In the control group there was considerably less new bone and some adipose tissue was observed at the defect margins 8 weeks after implantation. CONCLUSIONS: Autogenous cells transfected with hBMP-2 promote bone formation in osteoporotic rats. BMSC-mediated BMP-2 gene therapy used in conjunction with bone tissue engineering may be used to successfully treat bone defects in osteoporotic rats. This method provides a powerful tool for bone regeneration and other tissue engineering.     

Prospective of Genomics in Revealing Transmission, Reassortment and Evolution of Wildlife-Borne Avian Influenza A (H5N1) Viruses

The outbreak of highly pathogenic avian influenza (HPAI) H5N1 disease has led to significant loss of poultry and wild life and case fatality rates in humans of 60%. Wild birds are natural hosts for all avian influenza virus subtypes and over120 bird species have been reported with evidence of H5N1 infection. Influenza A viruses possess a segmented RNA genome and are characterized by frequently occurring genetic reassortment events, which play a very important role in virus evolution and the spread of novel gene constellations in immunologically naïve human and animal populations. Phylogenetic analysis of whole genome or sub-genomic sequences is a standard means for delineating genetic variation, novel reassortment events, and surveillance to trace the global transmission pathways. In this paper, special emphasis is given to the transmission and circulation of H5N1 among wild life populations, and to the reassortment events that are associated with inter-host transmission of the H5N1 viruses when they infect different hosts, such as birds, pigs and humans. In addition, we review the inter-subtype reassortment of the viral segments encoding inner proteins between the H5N1 viruses and viruses of other subtypes, such as H9N2 and H6N1. Finally, we highlight the usefulness of genomic sequences in molecular epidemiological analysis of HPAI H5N1 and the technical limitations in existing analytical methods that hinder them from playing a greater role in virological research.