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991.
Oztürk N Kao YT Selby CP Kavakli IH Partch CL Zhong D Sancar A 《Biochemistry》2008,47(39):10255-10261
The photolyase/cryptochrome family is a large family of flavoproteins that encompasses DNA repair proteins, photolyases, and cryptochromes that regulate blue-light-dependent growth and development in plants, and light-dependent and light-independent circadian clock setting in animals. Phylogenetic analysis has revealed a new class of the family, named type III photolyase, which cosegregates with plant cryptochromes. Here we describe the isolation and characterization of a type III photolyase from Caulobacter crescentus. Spectroscopic analysis shows that the enzyme contains both the methenyl tetrahydrofolate photoantenna and the FAD catalytic cofactor. Biochemical analysis shows that it is a bona fide photolyase that repairs cyclobutane pyrimidine dimers. Mutation of an active site Trp to Arg disrupts FAD binding with no measurable effect on MTHF binding. Using enzyme preparations that contain either both chromophores or only folate, we were able to determine the efficiency and rate of transfer of energy from MTHF to FAD. 相似文献
992.
罗氏沼虾肌肉白浊病病原研究 总被引:14,自引:0,他引:14
从患白浊病的罗氏沼虾虾苗病灶分离到10多株菌株,其中一株经人工感染证实为病原菌,经ATB Expression自动细菌鉴定仪测试,为木糖葡萄球菌,其主要特性为拟球状(直径0.8-1.1μm),革兰氏阳性,无鞭毛,无荚膜,不产生芽孢,发酵葡萄糖、果糖、麦芽糖、乳糖、海藻糖、阿拉伯糖、蔗糖及甘露醇产酸,β-半乳糖甘酶、过氧化氢酶及脲酶阳性,氧化酶,血浆凝固醇、V-P反应,精氨酸双水解酶、鸟氨酸脱羧酶均为阴性。最适生长温度、盐度和pH值范围分别为28-37℃、0%-2%、6-9,对红霉素、卡那毒素、头孢氨苄及庆大霉素等药物敏感。 相似文献
993.
UV—B辐射增强对植物光合作用的影响及植物的相关适应性研究 总被引:32,自引:6,他引:26
综述了UV-B辐射增强对植物光合作用的影响,植物对光破坏的响应与适应性方面的国内外研究进展,许多研究表明UV-B辐射增强对植物具有破坏作用能引起植物光抑制,光氧化和光损伤,植物依靠自身修复系统而对其破坏又具有一定的适应性。 相似文献
994.
布氏田鼠摄食量、累积摄食量与日龄的关系 总被引:5,自引:3,他引:2
布氏田鼠 (Microtusbrandti)是内蒙古典型草原区的主要害鼠 ,其危害方式主要表现之一为与牲畜争夺牧草资源[1,4 ,11,12 ] 。准确地测定其日食量(DFC)与累积摄食量 (CFC) ,对于定量地衡量该鼠的危害程度 ,进一步推算鼠害防治的经济阈值有着重要的意义。一些学者着手有关该鼠食性与食量 ,以及非取食性牧草消耗量的研究工作[3 ,7~ 9] 。但由于受实验手段的限制 ,一直未能解决布氏田鼠日食量、累积摄食量与田鼠年龄的关系等难题。本文以测定典型草原区布氏田鼠日食量与体重的关系 ,结合布氏田鼠体重与年龄的数学关系方程 … 相似文献
995.
布氏田鼠对主要贮草种类的选储嗜好 总被引:2,自引:0,他引:2
布氏田鼠 (Microtusbrandti)为内蒙古典型草原区的主要害鼠 ,其危害主要表现在与牲畜争夺牧草资源[1,3 ,5,6,9] 。该鼠属不冬眠种类 ,冬季主要以洞群贮草仓库中的贮草为食 ,因此 ,研究其秋季集群的贮草习性 ,对于分析和了解该鼠的越冬生态特征具有一定的意义。有关布氏田鼠的贮草习性 ,已有一些报道[4 ,7] 。研究表明 ,在羊草 冷蒿 隐子草草场 ,布氏田鼠的越冬贮草以蒿属 (Artemisia)植物为主 ,其中冷蒿 (A .frigida)与黄蒿 (A .scoparia)占贮草比例超过 90 % [2 ] ,但上述研究均未涉及布氏田鼠… 相似文献
996.
997.
Organic carbon content and distribution in soils under different land uses in tropical and subtropical China 总被引:17,自引:0,他引:17
Tropical and subtropical China comprises a land area of about 215 Mha, but reports on its soil C storage and contents are limited. The objective of this study was to investigate the C density, stocks and distribution in soils of this region under different land uses by using soil species data from the Second National Soil Survey and the Vegetation Map of the People's Republic of China (1:4 M). It was estimated that there is a total of about 28.7±8.2 Pg organic C stored in the upper 1 m of soils of the entire region. Changes of C content (C) with depth (D) were observed following a relationship of C = (c + D)/ (a + bD), or C = 1/(a + bD). Of the various patterns of land uses in the region, soil C density was generally higher in the west than in the east, and while small differences were found in croplands, there were large variations in natural soils. In the west, the C density of meadow and herbaceous swamp soil was the highest (about 40 kg C/m2), followed by coniferous and broad-leaf forest soils (19.6 and 19.2 kg C/m2, respectively). The C density of paddy, bush and coppice forest soils showed a density of 12.6 and 14.6 kg C/m2, respectively. Upland and grass-savanah soils ranked the lowest (9.4 and 10.5 kg C/m2, respectively). In the east, meadow and herbaceous swamp soil had the highest C density (25.2 kg C/m2), but differences in C density among soils under coniferous forest, broad-leaf forest, bush and coppice forest, and rice were small, varying from 10.2 to 11.4 kg C/m2. The C density of upland soil (7.2 kg C/m2), appeared a little higher than that of grass-savanah soil (6.3 kg C/m2). For the various land uses in the region, the C density estimation is accompanied by relatively large variations. 相似文献
998.
999.
The construction of inulin-assimilating and sorbitol-producing fusants was achieved by intergeneric protoplast fusion between Kluyveromyces sp. Y-85 and Saccharomyces cerevisiae E-15. The cells of parental strains were pretreated with 0.1% EDTA (w/v) and 2-mercaptoethanol (0.1%, v/v) and then exposed to 2.0% (w/v) Zymolase at 30 °C for 30–40 min. The optimized fusion condition demonstrated that with the presence of 30% (w/v) polyethylene glycol 6000 (PEG-6000) and 10 mM CaCl2 for 30 min, the fusion frequency reached 2.64 fusants/106 parental cells. The fusants were screened by different characters between two parental strains and further identified by DNA contents, inulinase activity and sorbitol productivity. One of the genetically stable fusants, Strain F27, reached a maximal sorbitol production of 4.87 g/100 ml under optimal fermentation condition. 相似文献
1000.
Guang-Hua Gao Wei Liu Ji-Xun Dai Jin-Feng Wang Zhong Hu Ying Zhang Da-Cheng Wang 《International journal of biological macromolecules》2001,29(4-5):251-258
The antifungal peptide from seeds of Phytolacca Americana (Pokeweed), designated PAFP-S hereinafter, is a recently found cationic peptide which consists of 38 amino acid residues and exihibites a broad spectrum of antifungal activity, including inhibition of certain saprophytic fungi and some plant pathogens. The secondary structure and three cysteine pairings have been investigated by 1H NMR analysis. The results show that the molecular scaffold of PAFP-S features a triple-stranded antiparallel β-sheet knotted by a typical disulfide bridge motif, which characterizes the knottin fold. CD spectroscopy indicates a high stability of the molecule in solution. Therefore, PAFP-S should be a new member of the knottin structural family and the first antifungal peptide that adopts the knottin-like fold. 相似文献