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91.
苏芸金杆菌液体深层发酵中用营养体接种二级发酵工艺和用二级发酵初期培养物作种子进行三级发酵工艺是可行的。试验结果表明:二级发酵种子罐营养体最佳移种茵龄为9h左右,此时营养体数量多、整齐、染色均匀,显微镜下菌体有折光点存在,三级接种营养体菌龄约为4h左右,菌体数量多,同步率高,少量染色不均匀。发酵液含菌数和发酵产品毒力均达到芽抱接种相同水平,但生产周期明显缩短4-5h,因此相应提高发酵罐生产能力20%。 相似文献
92.
DNaseⅠ超敏感位点的研究能够发现潜在的调控基因转录活化的位点,比较正常人外周血有核细胞,淋巴瘤细胞株P3HR1和人鼻咽癌低分化磷癌细胞株HOnE1和HNE2中Ha-ras-1瘤基因的DNaseⅠ超敏感位点发现,只有HONE1和HNE2细胞基因组中存在一个DNaseⅠ超敏感位点,位于第一个外显子上游0.37kb处,上述结果提示正常白细胞和P3HR1细胞中Ha-ras-1基因处于失活状态,而在鼻咽癌细胞基因组中则处于活化状态,它的活化可能与0.37kb处的DNA序列有密切的关系。 相似文献
93.
94.
This study investigated the compatibility of arbitrarily primed polymerase chain reaction (AP-PCR) and ribotyping in the characterization of Actinobacillus actinomycetemcomitans , a major pathogen in the mixed anaerobic microflora of human periodontitis. AP-PCR was performed directly on lysed bacterial colonies using a random-sequence 10-base oligonucleotide primer. Ribotyping was carried out by using purified bacterial chromosomal DNA digested with BglI. DNA fragments were separated electrophoretically, blotted onto a nylon membrane and hybridized with the plasmid pKK3535 containing the rRNA operon of Escherichia coli. The two genetic methods were evaluated on isolates from single individuals and from family members. Twelve AP-PCR types and 47 ribotypes were distinguished among 76 A. actinomycetemcomitans isolates of different serotypes. AP-PCR typing and ribotyping gave compatible results in 18 of 20 comparisons. Although AP-PCR detected less genetic heterogeneity in A. actinomycetemcomitans than ribotyping, the rapid and relatively simple AP-PCR technique seems to be sufficiently discriminative to be used in large scale epidemiological studies which preclude the application of the more laborious ribotyping technique. 相似文献
95.
96.
The role of heme-regulated eIF-2 alpha kinase (HRI) in the regulation of protein synthesis in rabbit reticulocytes is well documented. Inhibitors of protein synthesis with properties similar to those of HRI have been described in some nonerythroid cell types, but it has not yet been determined whether these eIF-2 alpha kinase activities are mediated by HRI or one or more as yet uncharacterized kinases. We have studied the expression of mRNA, polypeptide, and kinase activities of HRI in various tissues from both nonanemic and anemic rabbits. Our results indicate that HRI is expressed in an erythroid cell-specific manner. HRI is present in the bone marrow and peripheral blood of both nonanemic and anemic rabbits but not in any of the other tissues tested. HRI mRNA is present at low levels in uninduced mouse erythroleukemic (MEL) cells and human K562 cells and accumulates to higher levels upon induction. The accumulation of HRI mRNA in differentiating MEL cells is dependent upon the presence of heme. The addition of 3-amino-1,2,4-triazole (AT), an inhibitor of heme biosynthesis, to the induction medium markedly reduced HRI mRNA accumulation. Simultaneous addition of hemin and AT to the dimethyl sulfoxide induction medium largely prevented the inhibition of HRI mRNA induction by AT. These findings indicate that HRI is expressed in an erythroid cell-specific manner and that the major physiologic role of HRI is in adjusting the synthesis of globins to the availability of heme. 相似文献
97.
98.
Summary Partition and production of the extracellular chitinase from Serratia marcescens were studied in PEG/dextran aqueous two-phase systems. The enzyme partitions into the bottom phase and the cells segregate into the top phase. The best system is 2% (w/v) PEG 20000 and 5% (w/v) dextran T500. The cell growth and enzyme production kinetics are similar in the aqueous two-phase system and in the polymer-free reference system. However, the maximum enzyme concentration in the former system is 1.5 times that in the latter one. 相似文献
99.
Hydraulic properties of sphagnum peat moss and tuff (scoria) and their potential effects on water availability 总被引:2,自引:0,他引:2
The importance of macrostructure to root growth of ryegrass (L. perenne) seedlings sown on the soil surface was studied in two soils in which the macrostructure had resulted mainly from root growth
and macro-faunal activity. Sets of paired soil cores were used, one of each pair undisturbed and the other ground and repacked
to the field bulk density.
Undisturbed and repacked soils were first compared at equal water potentials in the range −1.9 to −300 kPa. At equal water
potential, the undisturbed soil always had the greater strength (penetration resistance), and root growth was always greater
in the repacked soil with no macrostructure than it was in the soil with macrostructure intact. At equal high strength (low
water potentials) it appeared that root growth was better when soils were structured. When strength was low (high water potentials),
root growth was better in the unstructured soil.
Soils were then compared during drying cycles over 21 days. The average rate at which roots grew to a depth of 60 mm, and
also the final percentage of plants with a root reaching 60 mm depth, was greatest in repacked soils without macrostructure.
The species of vegetation growing in the soil before the experiment affected root growth in undisturbed soil; growth was slower
where annual grasses and white clover had grown compared with soil which had supported a perennial grass.
It appears that relatively few roots locate and grow in the macrostructure. Other roots grow in the matrix, if it is soft
enough to be deformed by roots. Roots in the matrix of a structured soil grow more slowly than roots in structureless soil
of equal bulk density and water potential. The development of macrostructure in an otherwise structureless soil, of the type
studied, is of no advantage to most roots. However, once a macrostructure has developed, the few roots locating suitable macropores
are able to grow at low water potential when soil strength is high. The importance of macrostructure to establishing seedlings
in the field lies in rapid penetration of at least a few roots to a depth that escapes surface drying during seasonal drought.
ei]{gnB E}{fnClothier} 相似文献
100.
The effects of salicylic acid (SA) on ethylene biosynthesis in detached rice leaves were investigated. SA at pH 3.5 effectively inhibited ethylene production within 2 h of its application. It inhibited the conversion of ACC to ethylene, but did not affect the levels of ACC and conjugated ACC. Thus, the inhibitory effect of SA resulted from the inhibition of both synthesis of ACC and the conversion of ACC to ethylene.Abbreviations ACC
1-aminocyclopropane-1-carboxylic acid
- EFE
ethylene-forming enzyme
- SA
salicylic acid 相似文献