聚乙二醇处理大豆种子子叶中几种酶活性和可溶性蛋白含量的变化

聚乙二醇处理的大豆种子的异柠檬酸裂解酶、苹果酸脱氢酶、过氧化氢酶、超氧物歧化酶、酸性磷酸酶、碱性磷酸酶的活性明显高于受低温吸胀冷害的种子子叶的活性,相关的酶活性协同地增长,而蛋白质的含量没有明显的变化。这些酶活性的提高可能是渗透调控处理对细胞膜系统修补的结果。     

受白粉菌诱导大麦抗感等基因系蛋白质变化的双向电泳分析

分别对接种与否的大麦抗—感白粉病等基因系—叶期幼苗取材进行蛋白质双向电泳分析。结果表明,病原的侵入使抗—感两系在30Kd以下的低分子量区域的蛋白质发生了明显变化。接种48小时之后,抗病系在pH5.5、6.0、6.8及8.8附近出现了对照中所没有的蛋白质,而在pH6.0和8.8附近的蛋白质则较对照有减小的趋势;感病系在pH6.0附近蛋白质明显增多,在pH8.8处不仅在量上有大幅度提高,而且种类也有增加。结果还表明,抗—感系间在未接种的情况下双向电泳图谱也有差异,接种之后由于感病系在pH8.8处蛋白质的特异性合成,使抗—感两系间的差异缩小。     

阔叶红松林合理经营与土壤微生物活性

森林是可再生的自然资源,研究证明,阔叶红松林具有很高的生产力,因为它具有最优化的林分结构,这个结构特点,使它的复层异龄和针阔混交的群落结构影响下的土壤肥力较高,土壤微生物类群复杂,活动活跃,森林有机残体的分解与转化较快。系统内的氮、碳及无机养分的循环,能满足林木生长对养分不断增长的需要。系统内各个生态因子处于动态平衡状态。因此,为了不断提     

从我国成人手足口病患者疱液中首次分离出肠道病毒71型

本文报道了从我国手足口病(HFMD)患者疱液中肠道病毒71(E71)型H株的分离和鉴定。该株病毒可在原代人胚肺(HEL)细胞、MA104细胞、BSC细胞中生长繁殖,导致典型的肠道病毒CPE出现。将H株接种乳鼠后出现肢体麻痹、第6天开始死亡。电镜下可见感染H株的BSC细胞胞浆中出现大量的结晶状排列的成熟病毒颗粒,直径约25nm。患者双份血清中有对H株4倍增高的中和抗体存在。采用100抗体单位的抗Cox A5、7、9、16、E70、E71抗体和50抗体单位的LBM组合血清A-H以及抗E71BrCr株MeAb P27对H株进行中和试验时,H株可被抗E71血清和MeAb P27所中和。抗E71抗体对H株的最低有效中和作用为1.6抗体单位,MeAb P27对H株的有效中和作用是64抗体单位。其它血清则无此中和作用。然而,在鉴定过程中发现,高滴度的抗Cox A16抗体(200抗体单位以上)也显出有中和H株的作用,提示我们所分离的H株含有与Cox A16的型间共同抗原。     

An ecological study of soils in the Highlands of western Tibet I. Soils along an iso-altitudinal transect across western Tibet

A variety of soil studies were carried out along a 920-km transect running SE to NW from near the border with northern Nepal, through the Western Tibet Highlands (soils sampled at six locations at an elevation of about 4200 m), to the Takla Makan Desert. This transect, which parallels the China-India border, covers a range of aridities. Annual precipitation decreased exponentially (from 169 mm to ca. 50 mm) north-westwards along the transect. The humidity index also decreased exponentially to the desert zone in the Western Tibet Highlands, and decreased further in the Takla Makan Desert because of the high temperature in the latter area. Vegetation changed fromCaragana thorn-bush steppe, throughArtemisia sparse steppe, to desert. Soil properties were studied in relation to the humidity index. Organic C, total N, cation exchange capacity, and very low C/N ratio values (a maximum of 12.8) of soils decreased with the decrease in humidity index. In contrast, carbonate, pH (KCl), and sulfate increased exponentially. Available P and ammonia content were inversely related to the abundance of carbonate, whereas nitrate increased where carbonate was abundant. Illite was the most abundant of the clay minerals, followed in order by kaolinite and montmorillonite.     

沙门氏菌Ⅱ的三个新血清型

Eight cultures isolated from intestinal contents of reptiles were belonged to 3 new serotypes of Salmonella. They were all ducitol fermented, malonate utilized, but not attack lactose and salicin, no growth in KCN broth, ONPG negative. Therefore, they would be included in Salmonella II. They were all attacked by Felix phage O-I. Three represented strains were selected for antigen analysis. Their antigenic formula were identified as follows: S3194 Salmonella II 6,7:1,v:e,n,z15 S3196 Salmonella II 6, 7:y: e, n, z(1)5 S3195 Salmonella II 6, 8: e, h: 1,2 Among them, S3196 was indole positive belonging to a rare biotype. In addition, there were two other cultures as well as the formula of S3194, and three other cultures as well as the formula of S3196 (one of indole positive, two of indole negative).     

一种兴奋性氨基酸受体亚型

N-甲基-D-门冬氨酸(NMDA)受体是一种兴奋性氨基酸受体,广泛分布于许多脑区。该受体被激活后,突触后膜产生一长时程的兴奋性突触后电位,进而引起多种神经生物学效应或神经毒性作用。Ca~(2 )对于NMDA受体效应的产生具有重要意义。