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51.
52.
杂交水稻及其“三系”线粒体DNA的AP—PCR指纹图谱 总被引:22,自引:1,他引:22
为了研究水稻(Oryza sativa L.)细胞质雄性不育(CMS)与线粒体基因组的关系,应用AP-PCR 分析,用7 个任意单引物对6 种水稻品系线粒体DNA 进行了扩增。水稻线粒体DNA 的AP-PCR 产物可分为三种类型:(1)所有供试品系均能扩增的片段,它们代表了线粒体DNA 在进化上的保守性序列。有4 个引物检测到这类片段。(2)2 个以上水稻品系共同出现而在全部供试材料间存在差异的扩增片段,这类片段是检测水稻线粒体DNA多态性的主要来源。(3)一种细胞质类型所特有的扩增片段,从引物R2 和V5 的扩增产物中发现了这类片段,它们可能与CMS有关联。另外,WA型不育系珍汕97A 与其杂种之间在6 个引物的扩增图谱上均存在不同程度的差异,说明两者的线粒体DNA序列结构可能存在某种差别 相似文献
53.
Heterocyclic ketones as inhibitors of histone deacetylase 总被引:1,自引:0,他引:1
Vasudevan A Ji Z Frey RR Wada CK Steinman D Heyman HR Guo Y Curtin ML Guo J Li J Pease L Glaser KB Marcotte PA Bouska JJ Davidsen SK Michaelides MR 《Bioorganic & medicinal chemistry letters》2003,13(22):3909-3913
Several heterocyclic ketones were investigated as potential inhibitors of histone deacetylase. Nanomolar inhibitors such as 22 and 25 were obtained, the anti-proliferative activity of which were shown to be mediated by HDAC inhibition. 相似文献
54.
Yi-Rong Guo 《Analytical biochemistry》2009,389(1):32-181
Using a simple test for rapid identification and quantification of pesticide multiresidues in food and environmental samples is a long-cherished approach for practical monitoring purposes. Here two gold-based lateral-flow strips (strip A and strip B) were investigated for simultaneous detection of carbofuran and triazophos. For the strip A format, a bispecific monoclonal antibody (BsMcAb) against both carbofuran and triazophos was employed to prepare the immunogold probe. For the strip B format, anti-carbofuran monoclonal antibody (McAb) and anti-triazophos McAb separately labeled with colloidal gold were combined as detector reagents. By comparison of visual results from pesticide standard tests between the two formats, the strip B assay manifested higher sensitivities for both pesticides. Analysis of spiked water samples by the preferable strip indicated that the detection limits for carbofuran and triazophos were 32 and 4 μg/L, respectively. The strength of the portable one-step strip assay was in the simultaneous screening for two pesticides within a short time (8-10 min) without any equipment. 相似文献
55.
中药山豆根的研究进展 总被引:2,自引:1,他引:2
本文对中药山豆根进行了本草考证,并概括总结了半个世纪以来国内外对山豆根(广豆根)和北豆根在生药学研究、化学成分、药理作用与临床应用等方面的研究成果,为山豆根的进一步研究提供参考。全文附参考文献105篇。 相似文献
56.
Chenyang Han Yi Yang Qiaobing Guan Xiaoling Zhang Heping Shen Yongjia Sheng Jin Wang Xiaohong Zhou Wenyan Li Li Guo Qingcai Jiao 《Journal of cellular and molecular medicine》2020,24(14):8078-8090
The present study was designed to investigate the role of β‐amyloid (Aβ1‐42) in inducing neuronal pyroptosis and its mechanism. Mice cortical neurons (MCNs) were used in this study, LPS + Nigericin was used to induce pyroptosis in MCNs (positive control group), and Aβ1‐42 was used to interfere with MCNs. In addition, propidium iodide (PI) staining was used to examine cell permeability, lactate dehydrogenase (LDH) release assay was employed to detect cytotoxicity, immunofluorescence (IF) staining was used to investigate the expression level of the key protein GSDMD, Western blot was performed to detect the expression levels of key proteins, and enzyme‐linked immunosorbent assay (ELISA) was utilized to determine the expression levels of inflammatory factors in culture medium, including IL‐1β, IL‐18 and TNF‐α. Small interfering RNA (siRNA) was used to silence the mRNA expression of caspase‐1 and GSDMD, and Aβ1‐42 was used to induce pyroptosis, followed by investigation of the role of caspase‐1‐mediated GSDMD cleavage in pyroptosis. In addition, necrosulfonamide (NSA), an inhibitor of GSDMD oligomerization, was used for pre‐treatment, and Aβ1‐42 was subsequently used to observe the pyroptosis in MCNs. Finally, AAV9‐siRNA‐caspase‐1 was injected into the tail vein of APP/PS1 double transgenic mice (Alzheimer's disease mice) for caspase‐1 mRNA inhibition, followed by observation of behavioural changes in mice and measurement of the expression of inflammatory factors and pyroptosis‐related protein. As results, Aβ1‐42 could induce pyroptosis in MCNs, increase cell permeability and enhance LDH release, which were similar to the LPS + Nigericin‐induced pyroptosis. Meanwhile, the expression levels of cellular GSDMD and p30‐GSDMD were up‐regulated, the levels of NLRP3 inflammasome and GSDMD‐cleaved protein caspase‐1 were up‐regulated, and the levels of inflammatory factors in the medium were also up‐regulated. siRNA intervention in caspase‐1 or GSDMD inhibited Aβ1‐42‐induced pyroptosis, and NSA pre‐treatment also caused the similar inhibitory effects. The behavioural ability of Alzheimer's disease (AD) mice was relieved after the injection of AAV9‐siRNA‐caspase‐1, and the expression of pyroptosis‐related protein in the cortex and hippocampus was down‐regulated. In conclusion, Aβ1‐42 could induce pyroptosis by GSDMD protein, and NLRP3‐caspase‐1 signalling was an important signal to mediate GSDMD cleavage, which plays an important role in Aβ1‐42‐induced pyroptosis in neurons. Therefore, GSDMD is expected to be a novel therapeutic target for AD. 相似文献
57.
Lv K Guo Y Zhang Y Wang K Jia Y Sun S 《Biochemical and biophysical research communications》2008,374(1):101-105
The biological mechanism of a recent discovered association of type 2 diabetes with the ACAA-insertion/deletion polymorphism at the 3′UTR of the IGF2R gene has remained unclear. A very recently emerging novel polymorphic control layer by microRNAs (miRNAs) makes it possible to elucidate this issue. In this study, a prediction by web tools MicroInspector and miRanda demonstrated that DNA sequence polymorphism (DSPs) ACAA-insertion/deletion in IGF2R 3′UTR is located within the hsa-miR-657 and hsa-miR-453 binding sites. And luciferase reporter assay revealed that hsa-miR-657 acts directly at the 3′UTR of the IGF2R. Furthermore, ACAA-deletion exerted a further repression compared with ACAA-insertion, indicating that hsa-miR-657 regulates IGF2R gene expression in a polymorphic control manner. Importantly, we also demonstrated that hsa-miR-657 can translationally regulate the IGF2R expression levels in Hep G2 cells. Thus, our findings testify the possibility that the ACAA-insertion/deletion polymorphism may result in the change of IGF2R expression levels at least in part by hsa-miR-657-mediated regulation, contributing to the elucidation for the pathogenesis of type 2 diabetes and raise the possibility that miRNAs or in combination with functional DNA sequence polymorphism may be valuable in the treatment of human type 2 diabetes. 相似文献
58.
鲤鳃表面结构扫描电镜研究 总被引:31,自引:0,他引:31
鲤的鳃丝表面显示了由三边形一六边形不等的表皮细胞,呈现出各种图案式的结构。 相似文献
59.
60.
Soluble microbial products (SMPs) are considered as the main organic components in wastewater treatment plant effluent from
biological wastewater treatment systems. To investigate and explore SMP metabolism pathway for further treatment and control,
two innovative mechanistically based activated sludge models were developed by extension of activated sludge model no.3 (ASM3).
One was the model by combining SMP formation and degradation (ASM3-SMP model) processes with ASM3, and the other by combining
both SMP and simultaneous substrate storage and growth (SSSG) mechanisms with ASM3 (SSSG-ASM3-SMP model). The detailed schematic
modification and process supplements were introduced for comprehensively understanding all the mechanisms involved in the
activated sludge process. The evaluations of these two models were demonstrated by a laboratory-scale sequencing batch reactor
(SBR) operated under aerated/non-aerated conditions. The simulated and measured results indicated that SMP comprised about
83% of total soluble chemical oxygen demand (SCOD) in which biomass-associated products (BAPs) were predominant compared with
utilization-associated products (UAPs). It also elucidated that there should be a minimum SMP value as the reactive time increases
continuously and this conclusion could be used to optimize effluent SCOD in activated sludge processes. The comparative results
among ASM3, ASM3-SMP and SSSG-ASM3-SMP models and the experimental measurements (SCOD, ammonia and nitrate nitrogen) showed
clearly the best agreement with SSSG-ASM3-SMP simulation values (R = 0.993), strongly suggesting that both SMP formation and degradation and SSSG mechanisms are necessary in biologically activated
sludge modeling for municipal wastewater treatment. 相似文献