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61.
62.
Sun W Xing B Sun Y Du X Lu M Hao C Lu Z Mi W Wu S Wei H Gao X Zhu Y Jiang Y Qian X He F 《Molecular & cellular proteomics : MCP》2007,6(10):1798-1808
Hepatocellular carcinoma (HCC) is a highly malignant tumor, and chronic infection with hepatitis B virus is one of its major risk factors. To identify the proteins involved in HCC carcinogenesis, we used two-dimensional fluorescence DIGE to study the differentially expressed proteins in tumor and adjacent nontumor tissue samples. Samples from 12 hepatitis B virus-associated HCC patients were analyzed. A total of 61 spots were significantly up-regulated (ratio >/= 2, p = 0.01) in tumor samples, whereas 158 spots were down-regulated (ratio = -2, p = 0.01). Seventy-one gene products were identified among these spots. Members of the heat shock protein 70 and 90 families were simultaneously up-regulated, whereas metabolism-associated proteins were decreased in HCC samples. The down-regulation of mitochondrial and peroxisomal proteins in these results suggested loss of special organelle functions during HCC carcinogenesis. Four metabolic enzymes involved in the methylation cycle in the liver were down-regulated in HCC tissues, indicating S-adenosylmethionine deficiency in HCC. Two gene products, glyceraldehyde-3-phosphate dehydrogenase and formimidoyltransferase-cyclodeaminase, were identified from inversely altered spots, suggesting that different isoforms or post-translational modifications of these two proteins might play different roles in HCC. For the first time, the overexpression of Hcp70/Hsp90-organizing protein and heterogeneous nuclear ribonucleoproteins C1/C2 in HCC tissues was confirmed by Western blot and then by immunohistochemistry staining in 70 HCC samples, suggesting their potential as protein tumor markers. In summary, we profiled proteome alterations in HCC tissues, and these results may provide useful insights for understanding the mechanism involved in the process of HCC carcinogenesis. 相似文献
63.
氯沙坦对自发性高血压大鼠肾脏蛋白质表达谱的影响 总被引:1,自引:0,他引:1
目的:研究氯沙坦对自发性高血压大鼠肾脏组织蛋白质表达的影响。方法:采用蛋白质组学的技术,建立自发性高血压和氯沙坦干预后高血压大鼠肾脏的蛋白质二维凝胶电泳图谱,利用Imagemaster2Dv5.0软件分析蛋白点,并通过LC-MS/MS质谱分析和数据库检索鉴定差异蛋白质。结果:两组凝胶的平均蛋白点数分别为570±48、686±30。氯沙坦干预后有13个蛋白表达发生了显著变化,表达增强4个,表达降低4个,蛋白点消失5个。13个差异蛋白点进行质谱分析,鉴定出的7个蛋白质为Heat shock protein(Hsp)、Tubulin alpha-1chain、Transthyretin precursor、Liver regeneration-related protein LRRG03、Ezrin-radixin-moesin binding phosphoprotein50、Phosphoglycerate kinase1、Anionic trypsin I precursor。结论:这些差异表达的蛋白质可能在氯沙坦对高血压肾脏保护中发挥一定作用。 相似文献
64.
Meghann Ryan Peter Kochunov Laura M. Rowland Braxton D. Mitchell S. Andrea Wijtenburg Els Fieremans Jelle Veraart Dmitry S. Novikov Xiaoming Du Bhim Adhikari Feven Fisseha Heather Bruce Joshua Chiappelli Hemalatha Sampath Seth Ament Jeffrey O'Connell Alan R. Shuldiner L. Elliot Hong 《Obesity (Silver Spring, Md.)》2017,25(11):1876-1880
65.
S.N. Wang P. Xu H.Z. Tang J. Meng X.L. Liu J. Huang H. Chen Y. Du H.D. Blankespoor 《Biotechnology letters》2005,26(19):1493-1496
A Pseudomonas sp. grew with nicotine optimally 3 g l–1 and at 30 °C and pH 7. Nicotine was fully degraded within 10 h. The resting cells degraded nicotine in tobacco solid waste completely within 6 h in 0.02 m sodium phosphate buffer (pH 7) at maximally 56 mg nicotine h–1 g dry cell–1. 相似文献
66.
67.
Hong Zhao Lijie Yuan Dongli Zhu Banghao Sun Juan Du Jingyuan Wang 《Polish journal of microbiology》2022,71(2):173
To explore the role of gut microbiota in Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). Seventy fecal samples were collected, including 27 patients with GD, 27 with HT, and 16 samples from healthy volunteers. Chemiluminescence was used to detect thyroid function and autoantibodies (FT3, FT4, TSH, TRAb, TGAb, and TPOAb); thyroid ultrasound and 16S sequencing were used to analyze the bacteria in fecal samples; KEGG (Kyoto Encyclopedia of Genes and Genomes) and COG (Clusters of Orthologous Groups) were used to analyze the functional prediction and pathogenesis. The overall structure of gut microbiota in the GD and HT groups was significantly different from the healthy control group. Proteobacteria and Actinobacteria contents were the highest in the HT group. Compared to the control group, the GD and HT groups had a higher abundance of Erysipelotrichia, Cyanobacteria, and Ruminococcus_2 and lower levels of Bacillaceae and Megamonas. Further analysis of KEGG found that the “ABC transporter” metabolic pathway was highly correlated with the occurrence of GD and HT. COG analysis showed that the GD and HT groups were enriched in carbohydrate transport and metabolism compared to the healthy control group but not in amino acid transport and metabolism. Our data suggested that Bacillus, Blautia, and Ornithinimicrobium could be used as potential markers to distinguish GD and HT from the healthy population and that “ABC transporter” metabolic pathway may be involved in the pathogenesis of GD and HT. 相似文献
68.
69.
Liu Q He X Liu Y Du B Wang X Zhang W Jia P Dong J Ma J Wang X Li S Zhang H 《Biochemical and biophysical research communications》2008,377(3):775-779
Oxidative damage is an important mechanism in X-ray-induced cell death. Radiolysis of water molecules is a source of reactive oxygen species (ROS) that contribute to X-ray-induced cell death. In this study, we showed by ROS detection and a cell survival assay that NADPH oxidase has a very important role in X-ray-induced cell death. Under X-ray irradiation, the upregulation of the expression of NADPH oxidase membrane subunit gp91phox was dose-dependent. Meanwhile, the cytoplasmic subunit p47phox was translocated to the cell membrane and localized with p22phox and gp91phox to form reactive NADPH oxidase. Our data suggest, for the first time, that NADPH oxidase-mediated generation of ROS is an important contributor to X-ray-induced cell death. This suggests a new target for combined gene transfer and radiotherapy. 相似文献
70.
Xia Z Webster A Du F Piatkov K Ghislain M Varshavsky A 《The Journal of biological chemistry》2008,283(35):24011-24028