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961.
? With growing concerns over serious ecological problems, a particular challenge is to reveal the complex mechanisms underlying rapid expansion of invasive species. Ageratina adenophora is of particular interest in addressing this question. ? We used geographic information systems and logistic regression to identify the geographic and environmental factors contributing to the presence of A. adenophora. Join-count spatial statistics with reproduction mode examination were employed to elucidate the spatiotemporal dispersal mechanisms. ? Multiple factors have significantly contributed to the rapid expansion of A. adenophora. Its biological traits, favoring dispersal by water and wind coupled with local spatiotemporally heterogeneous geography and ecology, promote invasion downstream and upstream along river valleys, while other factors associated with human activities facilitate its invasion over high mountains and across river valleys, providing new scope for progressive invasions. We further identified an unusual invasion event of A. adenophora subsequent to a great flood that amplified its dispersal ability from vegetative propagules and seeds. ? These findings suggest that dynamic interactions of multiple factors in heterogeneous ecogeographical environments - a 'combinatorial' invasion mechanism - would generate an unexpected invasion rate of an alien species or a seemingly stochastic invasion event. 相似文献
962.
963.
Identification of a Nosema bombycis (Microsporidia) spore wall protein corresponding to spore phagocytosis 总被引:1,自引:0,他引:1
Life-cycle stages of the microsporidia Nosema bombycis, the pathogen causing silkworm pebrine, were separated and purified by an improved method of Percoll-gradient centrifugation. Soluble protein fractions of late sporoblasts (spore precursor cells) and mature spores were analysed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Protein spots were recovered from gels and analysed by mass spectrometry. The most abundant differential protein spot was identified by database search to be a hypothetical spore wall protein. Using immunoelectron microscopy, we demonstrated that HSWP5 is localized to the exospore of mature spores and renamed it as spore wall protein 5 (NbSWP5). Further spore phagocytosis assays indicated that NbSWP5 can protect spores from phagocytic uptake by cultured insect cells. This spore wall protein may function both for structural integrity and in modulating host cell invasion. 相似文献
964.
Guirong Qiao Renying Zhuo Mingying Liu Jing Jiang Haiying Li Wenmin Qiu Luanyin Pan Sheng lin Xiaoguo Zhang Zongxiu Sun 《Acta Physiologiae Plantarum》2011,33(3):691-696
Soil salinity is a serious problem worldwide. It is necessary to improve the salt tolerance of plants to avoid the progressive
deterioration of saline soil. We showed that the over-expression of AtNHX1 improves salt tolerance in a transgenic poplar (Populus deltoides CL × P. euramericana CL “NL895”) under mannose selection. Four transgenic poplar plants were obtained. Southern blot analysis showed that the
pmi gene had integrated into the genome of the poplar. RT-PCR confirmed that AtNHX1 could be expressed normally in the transgenic plants. When tested for salt tolerance by NaCl stress, we measured a 100%
increase in Na+ content in the three transgenic lines (T18, T50, T98) significantly higher than the 33% increase seen in wild-type plants.
The chlorophyll content of the transgenic plants was not altered significantly, while the chlorophyll content in the control
plants showed a small decrease. MDA content was decreased in the transgenic plants. These results show that the AtNHX1 gene may enhance salt tolerance due to increased vacuolar compartmentalization of sodium ions. 相似文献
965.
Gao C Xu X Zhang X Che B Ma C Qiu J Tao F Xu P 《Applied and environmental microbiology》2011,77(19):7080-7083
Chemoenzymatic synthesis of N-acetyl-d-neuraminic acid from N-acetyl-d-glucosamine using the spore surface-displayed N-acetyl-d-neuraminic acid aldolase at a high concentration (53.9 g liter(-1)) was achieved in this study. Thus, displaying a target enzyme on the surface of spores might be an alternative for integration of biocatalytic conversion into chemical synthesis. 相似文献
966.
GeoChip-based analysis of the functional gene diversity and metabolic potential of microbial communities in acid mine drainage 总被引:3,自引:0,他引:3
Xie J He Z Liu X Liu X Van Nostrand JD Deng Y Wu L Zhou J Qiu G 《Applied and environmental microbiology》2011,77(3):991-999
Acid mine drainage (AMD) is an extreme environment, usually with low pH and high concentrations of metals. Although the phylogenetic diversity of AMD microbial communities has been examined extensively, little is known about their functional gene diversity and metabolic potential. In this study, a comprehensive functional gene array (GeoChip 2.0) was used to analyze the functional diversity, composition, structure, and metabolic potential of AMD microbial communities from three copper mines in China. GeoChip data indicated that these microbial communities were functionally diverse as measured by the number of genes detected, gene overlapping, unique genes, and various diversity indices. Almost all key functional gene categories targeted by GeoChip 2.0 were detected in the AMD microbial communities, including carbon fixation, carbon degradation, methane generation, nitrogen fixation, nitrification, denitrification, ammonification, nitrogen reduction, sulfur metabolism, metal resistance, and organic contaminant degradation, which suggested that the functional gene diversity was higher than was previously thought. Mantel test results indicated that AMD microbial communities are shaped largely by surrounding environmental factors (e.g., S, Mg, and Cu). Functional genes (e.g., narG and norB) and several key functional processes (e.g., methane generation, ammonification, denitrification, sulfite reduction, and organic contaminant degradation) were significantly (P < 0.10) correlated with environmental variables. This study presents an overview of functional gene diversity and the structure of AMD microbial communities and also provides insights into our understanding of metabolic potential in AMD ecosystems. 相似文献
967.
968.
A total of 287 agricultural soil samples collected from 26 provinces or autonomous regions of China were tested on their ability
to suppress the conidial germination of nine biocontrol fungal agents. These soil samples showed great differences in the
degree to inhibit the germination of conidia (22.8% < mean inhibition rate < 97.5%), but all exhibited fungistatic activities
above the moderate levels (mean inhibition rate > 50%) to most of tested fungi. Ten soil samples that have stronger fungistatic
intensity (germination inhibition rate > 68.3%) to the target fungi, Trichoderma viride and Paecilomyces lilacinus, were selected to evaluate their soil actinobacteria involved fungistasis in soil. Of the 1,000 isolates from those soil
samples, 345 actinobacteria exhibited fungistatic activity to conidial germination of T. viride and P. lilacinus with germination inhibition rates higher than 10%. Sequences encoding 16S rRNA gene of the 345 actinobacteria were analyzed
by ARDRA and resulted 44 different ARDRA types. Fifty-six isolates, at least one from each unique ARDRA type, were selected
for 16S rDNA sequencing and phylogenetic analysis. Results indicated that the actinobacteria involved in the soil fungistasis
had close phylogenetic relationship with the members of Sterptomycetaceae, Microbacteriaceae, Micrococcaceae, and Nocardiacea. 相似文献
969.
970.
Qiu J Luo M Wang J Dong J Li H Leng B Zhang Q Dai X Zhang Y Niu X Deng X 《FEMS microbiology letters》2011,324(2):147-155
Staphylococcus aureus is a versatile pathogen that can cause life-threatening infections. The growing emergence of methicillin-resistant S.?aureus strains and a decrease in the discovery of new antibiotics warrant the search for new therapeutic targets to combat infections. Staphylococcus aureus produces many extracellular virulence factors that contribute to its pathogenicity. Therefore, targeting bacterial virulence as an alternative strategy to the development of new antimicrobials has gained great interest. α-Toxin is a 33.2-kDa, water-soluble, pore-forming toxin that is secreted by most S.?aureus strains. α-Toxin is essential for the pathogenesis of pneumonia, as strains lacking α-toxin display a profound defect in virulence. In this report, we demonstrate that isoalantolactone (IAL), a naturally occurring compound found in Inula helenium (Compositae), has no anti-S.?aureus activity as per MIC evaluation in vitro. However, IAL can markedly inhibit the expression of α-toxin in S.?aureus at very low concentrations. Furthermore, the in vivo data indicate that treatment with IAL protects mice from S.?aureus pneumonia. 相似文献