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71.
J. F. M. L. Seegers W. J. J. Meijer G. Venema S. Bron A. C. Zhao S. A. Khan 《Molecular & general genetics : MGG》1995,249(1):43-50
The single-strand origin (SSO) of the rolling-circle (RC), broad-host-range lactococcal plasmid pWVO1 was functionally characterized. The activity of this SSO in the conversion of single-stranded DNA to double-stranded DNA was tested both in vivo and in vitro. In addition, the effect of this SSO on plasmid maintenance was determined. The functional pWVO1 SSO comprises a 250 by region, containing two inverted repeats (IRs). The activity of each IR was tested, separately and in combination, in a plasmid derivative that was otherwise completely devoid of structures that might function as SSO. One of the IRs (IR 1) showed some homology with other previously described SSOs of the SSOA type, as well as with the conversion signal of the Escherichia coli phage X174. This IR was shown to have a partial, RNA polymerise-independent activity in complementary strand synthesis, both in vivo and in vitro. The second IR, which had no activity of its own, was required for full SSO activity, both in vivo and in vitro. The conversion of single-stranded DNA to the double-stranded form by the complete SSO was only partly sensitive to inhibition by rifampicin, indicating the existence of an RNA polymerase-independent pathway for this event. The results suggest that the pWVO1 SSO can be activated by two different routes: an RNA polymerise-dependent one (requiring the entire SSO), and an RNA polymerase-independent one (requiring only IR I). 相似文献
72.
定量生育期内植物光合碳在植物组织-土壤的分配规律,对于理解全球碳循环有着重要意义。采用~(13)C-CO_2脉冲标记结合室内培养,通过元素分析仪-稳定同位素联用(Flash HT-IRMS)分析植物各部分及土壤δ~(13)C值,比较了不同生育期下水稻光合碳在不同组织间的分配规律,并量化了水稻光合碳向土壤碳库的转移。结果表明:(1)水稻地上部和根系干物质量随水稻生育期的增加而呈现递增趋势,不同的生育期表现为:分蘖期拔节期抽穗期扬花期成熟期。而整个生育期的根冠比为0.2—0.4,分蘖期的根冠比最高,随着水稻生育期的增加而递减,到抽穗期以后根冠比稳定在0.2左右。(2)脉冲标记6h后,水稻地上部和地下部(根系)的δ~(13)C值在-25.52‰—-28.33‰,不同器官的δ~(13)C值存在明显分馏效应,且趋势基本一致,即茎杆(籽粒)叶片(根系);这种由于水稻生育期特性导致的各器官碳同位素分馏的现象,可用于指示不同生育期下水稻光合碳的分配和去向。(3)不同生育期~(13)C-光合碳在植物-土壤系统的分配规律不同,生长前期光合碳向根系及土壤中分配的比例高,具有较强的碳汇能力,而随生育期光合碳在根系及土壤中的分配比例呈下降趋势,但积累量不断增加。(4)不同生育期~(13)C光合碳在水稻-土壤系统中的分配比例差异明显。水稻分蘖期有近30%光合碳用于根系建成并部分通过根系分泌物进入土壤有机碳库(10%),而到成熟期则向籽粒中分配较多,而且光合碳在土壤中的分配比例也随生育期呈下降趋势。研究结果对稻田土壤有机碳循环过程和调控机制的揭示具有重要的理论意义。 相似文献
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Chen JG Xia JC Liang XT Pan K Wang W Lv L Zhao JJ Wang QJ Li YQ Chen SP He J Huang LX Ke ML Chen YB Ma HQ Zeng ZW Zhou ZW Chang AE Li Q 《International journal of biological sciences》2011,7(1):53-60
In this study, we characterized the intratumoral expression of IL-17 and CD8(+) TILs in gastric adenocarcinoma patients after resection and determined the correlation between the survival probability of gastric adenocarcinoma patients and the expression of IL-17 in tumor. Expression of IL-17 and CD8 was assessed by immunohistochemistry, and the prognostic effects of intratumoral IL-17 expression and CD8(+) TILs were evaluated by Cox regression and Kaplan-Meier analysis. Immunohistochemical detection revealed the presence of IL-17 and CD8(+) cells in gastric adenocarcinoma tissue samples (90.6%, 174 out of 192 patients and 96.9%, 186 out of 192 patients, respectively). We have also found that intratumoral IL-17 expression was significantly correlated with age (p=0.004) and that the number of CD8(+)TILs was significantly correlated with UICC staging (p=0.012) and the depth of tumor invasion (p=0.022). The five-year overall survival probability among patients intratumorally expressing higher levels of IL-17 was significantly better than those expressing lower levels of IL-17 (p=0.036). Multivariate Cox proportional hazard analyses revealed that intratumoral IL-17 expression (HR: 0.521; 95% CI: 0.329-0.823; p=0.005) was an independent factor affecting the five-year overall survival probability. We conclude that low levels of intratumoral IL-17 expression may indicate poor prognosis in gastric adenocarcinoma patients. 相似文献
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78.
激素对亚洲玉米螟幼虫滞育的影响*赵章武**曹梅讯(中国科学院上海昆虫研究所上海200025)亚洲玉米螟Ostriniafurnacalis是我国乃至亚洲地区的重要农业害虫之一。它具有兼性滞育的特点,年发生1~7代,以末龄老熟滞育幼虫越冬。来年春天终止... 相似文献
79.
Wei-liang Ye Jiang-bo Du Bang-le Zhang Ren Na Yan-feng Song Qi-bing Mei Ming-gao Zhao Si-yuan Zhou 《PloS one》2014,9(5)
A PEG-based, folate mediated, active tumor targeting drug delivery system using DOX-hyd-PEG-FA nanoparticles (NPs) were prepared. DOX-hyd-PEG-FA NPs showed a significantly faster DOX release in pH 5.0 medium than in pH 7.4 medium. Compared with DOX-hyd-PEG NPs, DOX-hyd-PEG-FA NPs increased the intracellular accumulation of DOX and showed a DOX translocation from lysosomes to nucleus. The cytotoxicity of DOX-hyd-PEG-FA NPs on KB cells was much higher than that of free DOX, DOX-ami-PEG-FA NPs and DOX-hyd-PEG NPs. The cytotoxicity of DOX-hyd-PEG-FA NPs on KB cells was attenuated in the presence of exogenous folic acid. The IC50 of DOX-hyd-PEG-FA NPs and DOX-hyd-PEG NPs on A549 cells showed no significant difference. After DOX-hyd-PEG-FA NPs were intravenously administered, the amount of DOX distributed in tumor tissue was significantly increased, while the amount of DOX distributed in heart was greatly decreased as compared with free DOX. Compared with free DOX, NPs yielded improved survival rate, prolonged life span, delayed tumor growth and reduced the cardiotoxicity in tumor bearing mice model. These results indicated that the acid sensitivity, passive and active tumor targeting abilities were likely to act synergistically to enhance the drug delivery efficiency of DOX-hyd-PEG-FA NPs. Therefore, DOX-hyd-PEG-FA NPs are a promising drug delivery system for targeted cancer therapy. 相似文献
80.