全文获取类型
收费全文 | 11630篇 |
免费 | 1114篇 |
国内免费 | 52篇 |
专业分类
12796篇 |
出版年
2024年 | 17篇 |
2023年 | 60篇 |
2022年 | 186篇 |
2021年 | 283篇 |
2020年 | 164篇 |
2019年 | 247篇 |
2018年 | 296篇 |
2017年 | 268篇 |
2016年 | 398篇 |
2015年 | 657篇 |
2014年 | 702篇 |
2013年 | 825篇 |
2012年 | 1067篇 |
2011年 | 1017篇 |
2010年 | 710篇 |
2009年 | 635篇 |
2008年 | 773篇 |
2007年 | 780篇 |
2006年 | 661篇 |
2005年 | 599篇 |
2004年 | 601篇 |
2003年 | 484篇 |
2002年 | 470篇 |
2001年 | 79篇 |
2000年 | 76篇 |
1999年 | 98篇 |
1998年 | 103篇 |
1997年 | 73篇 |
1996年 | 48篇 |
1995年 | 43篇 |
1994年 | 42篇 |
1993年 | 31篇 |
1992年 | 25篇 |
1991年 | 27篇 |
1990年 | 21篇 |
1989年 | 25篇 |
1988年 | 16篇 |
1987年 | 21篇 |
1986年 | 16篇 |
1985年 | 16篇 |
1984年 | 17篇 |
1983年 | 15篇 |
1982年 | 12篇 |
1981年 | 13篇 |
1979年 | 7篇 |
1977年 | 8篇 |
1975年 | 6篇 |
1974年 | 14篇 |
1973年 | 5篇 |
1967年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
Clotfelter ED Pedersen AB Cranford JA Ram N Snajdr EA Nolan V Ketterson ED 《Oecologia》2007,154(3):493-503
Resource pulses can have cascading effects on the dynamics of multiple trophic levels. Acorn mast is a pulsed resource in
oak-dominated forests that has significant direct effects on acorn predators and indirect effects on their predators, prey,
and pathogens. We evaluated changes in acorn mast, rodent abundance, raptor abundance, and reproductive success of a ground-nesting
songbird over a 24-year period (1980–2004) in the southern Appalachian Mountains in an effort to determine the relationships
among the four trophic levels. In particular, we examined the following: acorn mast from red oaks (Quercus rubra) and white oaks (Q. alba), abundance of white-footed mice (Peromyscus leucopus) and deer mice (P. maniculatus), population estimates of seven raptor species from three feeding guilds, and nest failure and number of juveniles of dark-eyed
juncos (Junco hyemalis). Finally, we recorded seasonal temperature and precipitation to determine the effects of weather on each trophic level.
We found that weather patterns had delayed effects of up to 3 years on these trophic interactions. Variation in acorn mast,
the keystone resource in this community, was explained by weather conditions as far back as 2 years before the mast event.
Acorn mast, in turn, was a strongly positive predictor of rodent abundance the following year, whereas spring and summer temperature
and raptor abundance negatively affected rodent abundance. Dark-eyed junco nests were more likely to fail in years in which
there were more rodents and raptors. Nest failure rate was a strong predictor of the number of juvenile juncos caught at the
end of the summer. Our results improve our understanding of the complex ecological interactions in oak-dominated forests by
illustrating the importance of abiotic and biotic factors at different trophic levels.
Ethan D. Clotfelter and Amy B. Pedersen contributed equally to the writing of this paper. 相似文献
72.
Stan D. Wullschleger Amy L. Breen Colleen M. Iversen Matthew S. Olson Torgny Näsholm Ulrika Ganeteg Matthew D. Wallenstein David J. Weston 《Molecular ecology》2015,24(10):2301-2309
Molecular ecology is poised to tackle a host of interesting questions in the coming years. The Arctic provides a unique and rapidly changing environment with a suite of emerging research needs that can be addressed through genetics and genomics. Here we highlight recent research on boreal and tundra ecosystems and put forth a series of questions related to plant and microbial responses to climate change that can benefit from technologies and analytical approaches contained within the molecular ecologist's toolbox. These questions include understanding (i) the mechanisms of plant acquisition and uptake of N in cold soils, (ii) how these processes are mediated by root traits, (iii) the role played by the plant microbiome in cycling C and nutrients within high‐latitude ecosystems and (iv) plant adaptation to extreme Arctic climates. We highlight how contributions can be made in these areas through studies that target model and nonmodel organisms and emphasize that the sequencing of the Populus and Salix genomes provides a valuable resource for scientific discoveries related to the plant microbiome and plant adaptation in the Arctic. Moreover, there exists an exciting role to play in model development, including incorporating genetic and evolutionary knowledge into ecosystem and Earth System Models. In this regard, the molecular ecologist provides a valuable perspective on plant genetics as a driver for community biodiversity, and how ecological and evolutionary forces govern community dynamics in a rapidly changing climate. 相似文献
73.
Yong-Hun Park Sang Keun Ha Inwook Choi Kyu Sik Kim Jeryang Park Nakwon Choi Bumsang Kim Jong Hwan Sung 《Biotechnology and Bioprocess Engineering》2016,21(1):110-118
Transdermal drug delivery system (TDDS) may provide a more reliable method of drug delivery than oral delivery by avoiding gut absorption and first-pass metabolism, but needs a method for efficiently crossing the epidermal barrier. To enhance the delivery through the skin, we have developed a biocompatible, dissolvable microneedle array made from carboxymethyl cellulose (CMC). Using laser ablation for creating the mold greatly improved the efficiency and reduced the cost of microneedle fabrication. Mixing CMC with amylopectin (AP) enhanced the mechanical and tunable dissolution properties of the microneedle for controlled release of model compounds. Using the CMC microneedle array, we observed significant enhancement in the skin permeability of a fluorescent model compound, and also increase in the anti-oxidant activity of ascorbic acid after crossing the skin. Our dissolvable microneedle array provides a new and biocompatible method for delivery of drugs and cosmetic compounds through the skin. 相似文献
74.
Wood anatomy and carbon‐isotope discrimination support long‐term hydraulic deterioration as a major cause of drought‐induced dieback 下载免费PDF全文
Elena Pellizzari J. Julio Camarero Antonio Gazol Gabriel Sangüesa‐Barreda Marco Carrer 《Global Change Biology》2016,22(6):2125-2137
Hydraulic impairment due to xylem embolism and carbon starvation are the two proposed mechanisms explaining drought‐induced forest dieback and tree death. Here, we evaluate the relative role played by these two mechanisms in the long‐term by quantifying wood‐anatomical traits (tracheid size and area of parenchyma rays) and estimating the intrinsic water‐use efficiency (iWUE) from carbon isotopic discrimination. We selected silver fir and Scots pine stands in NE Spain with ongoing dieback processes and compared trees showing contrasting vigour (declining vs nondeclining trees). In both species earlywood tracheids in declining trees showed smaller lumen area with thicker cell wall, inducing a lower theoretical hydraulic conductivity. Parenchyma ray area was similar between the two vigour classes. Wet spring and summer conditions promoted the formation of larger lumen areas, particularly in the case of nondeclining trees. Declining silver firs presented a lower iWUE than conspecific nondeclining trees, but the reverse pattern was observed in Scots pine. The described patterns in wood anatomical traits and iWUE are coherent with a long‐lasting deterioration of the hydraulic system in declining trees prior to their dieback. Retrospective quantifications of lumen area permit to forecast dieback in declining trees 2–5 decades before growth decline started. Wood anatomical traits provide a robust tool to reconstruct the long‐term capacity of trees to withstand drought‐induced dieback. 相似文献
75.
Proteome profiling of the inclusion body (IB) fraction of recombinant proteins produced in Escherichia coli suggested that two small heat shock proteins, IbpA and IbpB, are the major proteins associated with IBs. In this study, we demonstrate that IbpA and IbpB facilitate the production of recombinant proteins in E. coli and play important roles in protecting recombinant proteins from degradation by cytoplasmic proteases. We examined the cytosolic production, and Tat- or Sec-dependent secretion of the enhanced green fluorescent protein (EGFP) in wild type, ibpAB(-) mutant, and ibpAB-amplified E. coli strains. Analysis of fluorescence histograms and confocal microscopic imaging revealed that over-expression of the ibpA and/or ibpB genes enhanced cytosolic EGFP production whereas knocking out the ibpAB genes enhanced secretory production. This strategy seems to be generally applicable as it was successfully employed for the enhanced cytosolic or secretory production of several other recombinant proteins in E. coli. 相似文献
76.
The initiative role of XPC protein in cisplatin DNA damaging treatment-mediated cell cycle regulation 总被引:5,自引:0,他引:5
Wang G Chuang L Zhang X Colton S Dombkowski A Reiners J Diakiw A Xu XS 《Nucleic acids research》2004,32(7):2231-2240
XPC is an important DNA damage recognition protein involved in DNA nucleotide excision repair. We have studied the role of the XPC protein in cisplatin treatment-mediated cell cycle regulation. Through the comparison of microarray data obtained from human normal fibroblasts and two individual XPC-defective cell lines, 486 genes were identified as XPC-responsive genes in the cisplatin treatment (with a minimal 1.5-fold change) and 297 of these genes were further mapped to biological pathways and gene ontologies. The cell cycle and cell proliferation-related genes were the most affected genes by the XPC defect in the cisplatin treatment. Many other cellular function genes were also affected by the XPC defect in the treatment. Western blot hybridization results revealed that the XPC defect reduced the p53 responses to the cisplatin treatment. The ability to activate caspase-3 was also attenuated in the XPC cells with the treatment. These results suggest that the XPC protein plays a critical role in initiating the cisplatin DNA damaging treatment-mediated signal transduction process, resulting in activation of the p53 pathway and cell cycle arrest that allow DNA repair and apoptosis to take place. These results reveal an important role of the XPC protein in the cancer prevention. 相似文献
77.
Suk Hee Lee Suk Oh Young Il Kim Sang Cheol Jun Sang Sup So Hwan Gyu Kim 《Biotechnology and Bioprocess Engineering》2007,12(2):100-105
Phellinus linteus is a fungus which is found primarily in tropical regions of the Americas, Africa, and Asia.P. linteus has been used in traditional medical practice for the treatment of arthritis, liver damage and cancer. Angiogenesis is a
process that involves migration, proliferation and cell differentiation, as well as the formation of new capillary structures.
The anti-angiogenic activities evidenced by natural compounds may actually be a critical effect for the inhibition of angiogenesis-dependent
disease by these agents via the blockage of vascular development. This study assessed the effects of water extracts fromP. linteus (Phellinus extracts) on primary cultured porcine coronary artery endothelial cells (PCAECs).Phellinus extracts induced no changes in DNA synthesis or cell numbers, but inhibited the migration of PCAECs.Phellinus extracts also induced a reduction in the secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9. Our results show that,
in endothelial cells,Phellinus extracts may inhibit angiogenesis by reducing levels of MMP-2 and MMP-9 secretion. 相似文献
78.
79.
In-Gyun Lee Sang Jae Lee Susanna Chae Ki-Young Lee Ji-Hun Kim Bong-Jin Lee 《Nucleic acids research》2015,43(15):7624-7637
Toxin-antitoxin (TA) systems play important roles in bacterial physiology, such as multidrug tolerance, biofilm formation, and arrest of cellular growth under stress conditions. To develop novel antimicrobial agents against tuberculosis, we focused on VapBC systems, which encompass more than half of TA systems in Mycobacterium tuberculosis. Here, we report that theMycobacterium tuberculosis VapC30 toxin regulates cellular growth through both magnesium and manganese ion-dependent ribonuclease activity and is inhibited by the cognate VapB30 antitoxin. We also determined the 2.7-Å resolution crystal structure of the M. tuberculosis VapBC30 complex, which revealed a novel process of inactivation of the VapC30 toxin via swapped blocking by the VapB30 antitoxin. Our study on M. tuberculosis VapBC30 leads us to design two kinds of VapB30 and VapC30-based novel peptides which successfully disrupt the toxin-antitoxin complex and thus activate the ribonuclease activity of the VapC30 toxin. Our discovery herein possibly paves the way to treat tuberculosis for next generation. 相似文献
80.
William K. Cornwell William D. Pearse Rhiannon L. Dalrymple Amy E. Zanne 《Ecography》2019,42(11):1819-1831
The era of big biodiversity data has led to rapid, exciting advances in the theoretical and applied biological, ecological and conservation sciences. While large genetic, geographic and trait databases are available, these are neither complete nor random samples of the globe. Gaps and biases in these databases reduce our inferential and predictive power, and this incompleteness is even more worrisome because we are ignorant of both its kind and magnitude. We performed a comprehensive examination of the taxonomic and spatial sampling in the most complete current databases for plant genes, locations and functional traits. To do this, we downloaded data from The Plant List (taxonomy), the Global Biodiversity Information Facility (locations), TRY (traits) and GenBank (genes). Only 17.7% of the world's described and accepted land plant species feature in all three databases, meaning that more than 82% of known plant biodiversity lacks representation in at least one database. Species coverage is highest for location data and lowest for genetic data. Bryophytes and orchids stand out taxonomically and the equatorial region stands out spatially as poorly represented in all databases. We have highlighted a number of clades and regions about which we know little functionally, spatially and genetically, on which we should set research targets. The scientific community should recognize and reward the significant value, both for biodiversity science and conservation, of filling in these gaps in our knowledge of the plant tree of life. 相似文献