全文获取类型
收费全文 | 92137篇 |
免费 | 6749篇 |
国内免费 | 6310篇 |
出版年
2024年 | 129篇 |
2023年 | 1102篇 |
2022年 | 2671篇 |
2021年 | 4846篇 |
2020年 | 3198篇 |
2019年 | 4019篇 |
2018年 | 3960篇 |
2017年 | 2868篇 |
2016年 | 4056篇 |
2015年 | 5849篇 |
2014年 | 6889篇 |
2013年 | 7254篇 |
2012年 | 8501篇 |
2011年 | 7746篇 |
2010年 | 4485篇 |
2009年 | 4189篇 |
2008年 | 4778篇 |
2007年 | 4149篇 |
2006年 | 3533篇 |
2005年 | 2822篇 |
2004年 | 2314篇 |
2003年 | 2107篇 |
2002年 | 1698篇 |
2001年 | 1471篇 |
2000年 | 1342篇 |
1999年 | 1410篇 |
1998年 | 821篇 |
1997年 | 893篇 |
1996年 | 814篇 |
1995年 | 776篇 |
1994年 | 675篇 |
1993年 | 572篇 |
1992年 | 684篇 |
1991年 | 538篇 |
1990年 | 455篇 |
1989年 | 331篇 |
1988年 | 279篇 |
1987年 | 219篇 |
1986年 | 185篇 |
1985年 | 210篇 |
1984年 | 124篇 |
1983年 | 118篇 |
1982年 | 54篇 |
1981年 | 23篇 |
1980年 | 20篇 |
1979年 | 18篇 |
1976年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 906 毫秒
811.
Boris C. Dunkov Dianzheng Zhang Kyril Choumarov Joy J. Winzerling John H. Law 《Archives of insect biochemistry and physiology》1995,29(3):293-307
Ferritin, an iron storage protein, was isolated from larvae and pupae of Aedes aegypti grown in an iron-rich medium. Mosquito ferritin is a high molecular weight protein composed of several different, relatively small, subunits. Subunits of molecular mass 24, 26, and 28 kDa are equally abundant, while that of 30 kDa is present only in small amounts. The N-terminal sequence of the 24 and 26 kDa subunits are identical for the first 30 amino acids, while that of the 28 kDa subunit differs. Studies using antiserum raised against a subunit mixture showed that the ferritin subunits were present in larvae, pupae, and adult females, and were increased in animals exposed to excess iron. The antiserum also was used to screen a cDNA library from unfed adult female mosquitoes. Nine clones were obtained that differed only in a 27 bp insertion in the 3′ end. Rapid amplification of cDNA ends (RACE) was used to obtain the complete protein coding sequence. A putative iron-responsive element (IRE) is present in the 5′-untranslated region. The deduced amino acid sequence shows a typical leader sequence, consistent with the fact that most insect ferritins are secreted, rather than cytoplasmic proteins. The sequence encodes a mature polypeptide of 20,566 molecular weight, smaller than the estimated size of any of the subunits. However, the sequence exactly matches the N-terminal sequences of the 24 and 26 kDa subunits as determined by Edman degradation. Of the known ferritin sequences, that of the mosquito is most similar to that of somatic cells of a snail. © 1995 Wiley-Liss, Inc. 相似文献
812.
The expression of biologically active human p53 in Leishmania cells: a novel eukaryotic system to produce recombinant proteins. 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Nucleic acids research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We have investigated the use of Leishmania cells as a novel eukaryotic expression system for the production of recombinant protein. These cells are easy to maintain, requiring no CO2 incubator or shaker, and can be grown in standard tissue culture media. Leishmania cells can be readily transfected with plasmid DNA by electroporation and transformants selected with antibiotic resistance. Recent studies have shown that it is possible to express foreign genes in Leishmania for the purpose of understanding the biology of this protozoan cell. In the present study we report the use of this system as a means of producing a biologically functional human p53 protein. The conformation of the p53 protein is critical for its ability to bind specific DNA sequences. It is demonstrated that Leishmania-synthesized human p53 is phosphorylated and can bind specifically to its enhancer DNA sequence. These data demonstrate that Leishmania may represent a simple eukaryotic expression system for the production of biologically active recombinant proteins. 相似文献
813.
Narendra Tuteja Ning Wu Huang Doris Skopac Renu Tuteja Sara Hrvatic Jianwen Zhang Sandor Pongor Grard Joseph Christian Faucher Franois Amalric Arturo Falaschi 《Gene》1995,160(2):143-148
The cDNA encoding human DNA helicase IV (HDH IV), a 100-kDa protein which unwinds DNA in the 5′ to 3′ direction with respect to the bound strand, was cloned and sequenced. It was found to be identical to the human cDNA encoding nucleolin, a ubiquitous eukaryotic protein essential for pre-ribosome assembly. HDH IV/nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Phosphorylation of HDH IV/nucleolin by cdc2 kinase and casein kinase II enhanced its unwinding activity in an additive way. The Gly-rich C-terminal domain possesses a limited ATP-dependent duplex-unwinding activity which contributes to the helicase activity of HDH IV/nucleolin. 相似文献
814.
815.
816.
817.
A. Mezzelani Y. Zhang L. Redaelli B. Castiglioni P. Leone J. L. Williams S. Solinas Toldo G. Wigger R. Fries L. Ferretti 《Mammalian genome》1995,6(9):629-635
Gene mapping in cattle has progressed rapidly in recent years largely owing to the introduction of powerful genetic markers, such as the microsatellites, and through advances in physical mapping techniques such as synteny mapping and fluorescence in situ hybridization (FISH). Microsatellite markers are often not physically mapped because they are generally isolated from small insert plasmid libraries, which makes their chromosomal localization inefficient. In this report we describe the FISH mapping of a large group of cosmid-derived bovine microsatellite markers, as our contribution to the European mapping initiative, BovMap. One objective of BovMap is to develop a set of anchored loci for the cattle genome map.Two cosmid libraries were screened with probes corresponding to the (AC)
n
microsatellite motif. Positive clones were mapped by FISH, and then a subset was further analyzed by sequencing the region flanking the microsatellite repeat. In total, 58 clones were hybridized with chromosomes and identified loci on 22 of the 31 different bovine chromosomes. Three clones contained satellite DNA. Two or more markers were placed on 12 chromosomes. Sequencing of the microsatellites and flanking regions was performed directly from 43 cosmids, as previously reported (Ferretti et al. Anim. Genet. 25, 209–214, 1994). Primers were developed for 39 markers and used to describe the polymorphism associated with the corresponding loci. 相似文献
818.
Hong-Bo Qu Xian-En Zhang Wen-Wu Chui Shu-Zheng Zhang Gao-Xiang Li Fan Ouyang 《Biotechnology Techniques》1995,9(6):445-450
Summary A dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10 L sample size with recycled back-flow minimises any loss of the reaction medium. The production, growth and decay of glucose and maltose concentrations during starch hydrolysis under various enzymatic conditions can thus be closely monitored, making it useful for the study of the catalytic kinetics of amylases and in screening and analysing enzyme systems. 相似文献
819.
820.