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101.
“新工科”建设是以培养工程实践能力、创新能力和国际竞争力的高素质复合型人才为目标,双语课程已成为培养既懂专业又能进行国际交流的复合型人才的有效手段之一。然而,大多数双语课程教学效果不是很理想。本文在分析当前双语教学普遍存在问题的基础上,以环境生物技术双语课程为例,从教学模式构建、质控体系建立、教材选用和考核方式优化等方面探讨了改进的策略,从提高教师教学能力、学生学习热情和学校政策保障等方面总结了既往的经验和不足,以期为提高双语教学效果提供借鉴。 相似文献
102.
Tao Fang Wang Heng Wang Ai Fen Peng Qing Feng Luo Zhi Li Liu Rong Ping Zhou Song Gao Yang Zhou Wen Zhao Chen 《Biochemical and biophysical research communications》2013
FASN plays an important role in the malignant phenotype of various tumors. Our previous studies show that inhibition FASN could induce apoptosis and inhibit proliferation in human osteosarcoma (OS) cell in vivo and vitro. The aim in this study was to investigate the effect of inhibition FASN on the activity of HER2/PI3K/AKT axis and invasion and migration of OS cell. The expression of FASN, HER2 and p-HER2(Y1248) proteins was detected by immunohistochemistry in OS tissues from 24 patients with pulmonary metastatic disease, and the relationship between FASN and p-HER2 as well as HER2 was investigated. The results showed that there was a positive correlation between FASN and HER2 as well as p-HER2 protein expression. The U-2 OS cells were transfected with either the FASN specific RNAi plasmid or the negative control RNAi plasmid. FASN mRNA was measured by RT-PCR. Western blot assays was performed to examine the protein expression of FASN, HER2, p-HER2(Y1248), PI3K, Akt and p-Akt (Ser473). Migration and invasion of cells were investigated by wound healing and transwell invasion assays. The results showed that the activity of HER2/PI3K/AKT signaling pathway was suppressed by inhibiting FASN. Meanwhile, the U-2OS cells migration and invasion were also impaired by inhibiting the activity of FASN/HER2/PI3K/AKT. Our results indicated that inhibition of FASN suppresses OS cell invasion and migration via down-regulation of the “HER2/PI3K/AKT” axis in vitro. FASN blocker may be a new therapeutic strategy in OS management. 相似文献
103.
An IL-13 Promoter Polymorphism Associated with Liver Fibrosis in Patients with Schistosoma japonicum
104.
105.
Anatoli B. Meriin Martin Mense Jeff D. Colbert Feng Liang Hermann Bihler Nava Zaarur Kenneth L. Rock Michael Y. Sherman 《The Journal of biological chemistry》2012,287(41):34264-34272
Protein homeostasis depends on a balance of translation, folding, and degradation. Here, we demonstrate that mild inhibition of translation results in a dramatic and disproportional reduction in production of misfolded polypeptides in mammalian cells, suggesting an improved folding of newly synthesized proteins. Indeed, inhibition of translation elongation, which slightly attenuated levels of a copepod GFP mutant protein, significantly enhanced its function. In contrast, inhibition of translation initiation had minimal effects on copepod GFP folding. On the other hand, mild suppression of either translation elongation or initiation corrected folding defects of the disease-associated cystic fibrosis transmembrane conductance regulator mutant F508del. We propose that modulation of translation can be used as a novel approach to improve overall proteostasis in mammalian cells, as well as functions of disease-associated mutant proteins with folding deficiencies. 相似文献
106.
William K. K. Wu Minyi He Liang Zhao Xuegang Sun Hui Li Yong Jiang Yungao Yang Kang Peng 《Cell biochemistry and function》2012,30(4):271-278
Triptolide is a diterpenoid triepoxide derived from the traditional Chinese medical herb Tripterygium wilfordii. In the present study, we demonstrated that this phytochemical attenuated colon cancer growth in vitro and in vivo. Using a proteomic approach, we found that 14‐3‐3 epsilon, a cell cycle‐ and apoptosis‐related protein, was altered in colon cancer cells treated with triptolide. In this regard, triptolide induced cleavage and perinuclear translocation of 14‐3‐3 epsilon. Taken together, our findings suggest that triptolide may merit investigation as a potential therapeutic agent for colon cancer, and its anticancer action may be associated with alteration of 14‐3‐3 epsilon. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
107.
108.
Abstract: One-trial conditioning of the nudibranch mollusk Hermissenda produces short- and long-term changes in excitability (enhancement) of identified sensory neurons. To investigate the biochemical mechanisms underlying this example of plasticity, we have examined changes in protein phosphorylation at different times following the in vitro conditioning trial. Changes in the incorporation of 32 PO4 into proteins were determined using two-dimensional polyacrylamide gel electrophoresis, autoradiography, and densitometry. Conditioning resulted in increases in levels of several phosphoproteins, five of which, ranging in apparent molecular mass from 22 to 55 kDa, were chosen for analysis. The increased phosphorylation of the 46- and 55-kDa phosphoproteins detected 2 h postconditioning was significantly greater than the level of phosphorylation detected in an unpaired control group, indicating that long-term enhancement is pairing specific. Statistically significant increases in phosphorylation as compared with the control group that received only light were detected immediately after conditioning (5 min) for the 55-, 46-, and 22-kDa phosphoproteins, at 1 h for the 55- and 46-kDa phosphoproteins, and at 2 h for the 55-, 46-, and 22-kDa phosphoproteins. The 46- and 55-kDa phosphoproteins are putative structural proteins, and the 22-kDa phosphoprotein is proposed to be a protein kinase C substrate previously identified in Hermissenda following multitrial classical conditioning. Time-dependent increases in protein phosphorylation may contribute to the induction and maintenance of different memory stages expressed in sensory neurons after one-trial conditioning. 相似文献
109.
Kristina M. Fetalvero Yenyen Yu Margaret Goetschkes Guiqing Liang Reginald A. Valdez Ty Gould Ellen Triantafellow Sebastian Bergling Joseph Loureiro John Eash Victor Lin Jeffrey A. Porter Peter M. Finan Kenneth Walsh Yi Yang Xiaohong Mao Leon O. Murphy 《Molecular and cellular biology》2013,33(1):98-110
Autophagy is a vesicular trafficking pathway that regulates the degradation of aggregated proteins and damaged organelles. Initiation of autophagy requires several multiprotein signaling complexes, such as the ULK1 kinase complex and the Vps34 lipid kinase complex, which generates phosphatidylinositol 3-phosphate [PtdIns(3)P] on the forming autophagosomal membrane. Alterations in autophagy have been reported for various diseases, including myopathies. Here we show that skeletal muscle autophagy is compromised in mice deficient in the X-linked myotubular myopathy (XLMTM)-associated PtdIns(3)P phosphatase myotubularin (MTM1). Mtm1-deficient muscle displays several cellular abnormalities, including a profound increase in ubiquitin aggregates and abnormal mitochondria. Further, we show that Mtm1 deficiency is accompanied by activation of mTORC1 signaling, which persists even following starvation. In vivo pharmacological inhibition of mTOR is sufficient to normalize aberrant autophagy and improve muscle phenotypes in Mtm1 null mice. These results suggest that aberrant mTORC1 signaling and impaired autophagy are consequences of the loss of Mtm1 and may play a primary role in disease pathogenesis. 相似文献
110.
2004-2013年珠江流域植被变化及其胁迫分析 总被引:3,自引:0,他引:3
植被对区域气候调节、水文循环等有着重要作用,在近年来中国南部地区极端气候频发的背景下,研究植被变化及胁迫意义重大。以珠江流域为研究区,利用MODIS EVI分析了植被的变化规律,并通过美国军事气象卫星DMSP灯光数据和气象数据探讨分析了人类活动和自然环境对植被变化的胁迫。结果显示:2004年到2013年期间珠江流域内年平均EVI介于0.33-0.38之间,EVI从高到底依次是常绿阔叶林 > 混交林 > 多树的草地 > 常绿针叶林 > 草地,不同植被类型的EVI变化趋势基本一致,同一植被类型EVI年际变化较小,其中混交林和草地年际最大变化量分别为0.07和0.04,而常绿阔叶林、常绿针叶林和多树的草地年际最大变化量均为0.06;在2004年至2013年年间,城市化水平增长了约71%,其年发展变化与EVI的年变化趋势相反;通过对比分析发现珠江流域人类活动对植被变化影响高于自然环境,即DMSP灯光变化与EVI变化的相关系数明显高于气温和降水。 相似文献