全文获取类型
收费全文 | 39816篇 |
免费 | 16467篇 |
国内免费 | 1875篇 |
出版年
2024年 | 46篇 |
2023年 | 179篇 |
2022年 | 452篇 |
2021年 | 1120篇 |
2020年 | 2664篇 |
2019年 | 4295篇 |
2018年 | 4363篇 |
2017年 | 4483篇 |
2016年 | 4637篇 |
2015年 | 4844篇 |
2014年 | 4658篇 |
2013年 | 5160篇 |
2012年 | 3145篇 |
2011年 | 2706篇 |
2010年 | 3801篇 |
2009年 | 2472篇 |
2008年 | 1535篇 |
2007年 | 1067篇 |
2006年 | 960篇 |
2005年 | 911篇 |
2004年 | 844篇 |
2003年 | 796篇 |
2002年 | 700篇 |
2001年 | 542篇 |
2000年 | 434篇 |
1999年 | 337篇 |
1998年 | 170篇 |
1997年 | 138篇 |
1996年 | 115篇 |
1995年 | 77篇 |
1994年 | 76篇 |
1993年 | 55篇 |
1992年 | 63篇 |
1991年 | 44篇 |
1990年 | 45篇 |
1989年 | 43篇 |
1988年 | 23篇 |
1987年 | 24篇 |
1986年 | 8篇 |
1985年 | 10篇 |
1984年 | 13篇 |
1983年 | 8篇 |
1982年 | 11篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1976年 | 8篇 |
1975年 | 5篇 |
1973年 | 6篇 |
1972年 | 8篇 |
1969年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
992.
993.
Kathleen M. Maloney Jenny Ancca‐Juarez Renzo Salazar Katty Borrini‐Mayori Malwina Niemierko Joshua O. Yukich Cesar Naquira Joseph A. Keating Michael Z. Levy 《Journal of vector ecology》2013,38(1):6-11
The vector of Chagas disease, Triatoma infestans, is largely controlled by the household application of pyrethroid insecticides. Because effective, large‐scale insecticide application is costly and necessitates numerous trained personnel, alternative control techniques are badly needed. We compared the residual effect of organophosphate‐based insecticidal paint (Inesfly 5A IGR? (I5A)) to standard deltamethrin, and a negative control, against T. infestans in a simulated natural environment. We evaluated mortality, knockdown, and ability to take a blood meal among 5th instar nymphs. I5A paint caused significantly greater mortality at time points up to nine months compared to deltamethrin (Fisher's Exact Test, p < 0.01 in all instances). A year following application, mortality among nymphs in the I5A was similar to those in the deltamethrin (χ2 = 0.76, df=1, p < 0.76). At months 0 and 1 after application, fewer nymphs exposed to deltamethrin took a blood meal compared to insects exposed to paint (Fisher's Exact Tests, p < 0.01 and p < 0.01, respectively). Insecticidal paint may provide an easily‐applied means of protection against vectors of Chagas disease. 相似文献
994.
Nicole Wüppenhorst Friederike von Loewenich Beate Hobmaier Marianne Vetter‐Knoll Sona Mohadjer Manfred Kist 《Helicobacter》2013,18(1):1-5
Helicobacter felis belongs to the fastidious gastric non‐Helicobacter pylori helicobacter species that are typically found in the stomach of cats and dogs. These bacteria have the potential to colonize the human stomach and are then associated with gastritis, gastroduodenal ulcers, and MALT lymphoma. Strains cultured from the human stomach are rare. Here, we present the first isolation of H. felis from a gastric biopsy specimen of a 14‐year‐old girl who presented with persistent epigastric pain. The strain was cultured using our routine protocol for H. pylori and identified by phylogenetic analyses of partial urease AB and gyrB gene sequences. 相似文献
995.
Absolute Quantification of E1, Ubiquitin-Like Proteins and Nedd8–MLN4924 Adduct by Mass Spectrometry
Xiaofeng Yang James E. Brownell Qing Xu Fengying Zhu Jingya Ma Huay-Keng Loke Neil Rollins Teresa A. Soucy James J. Minissale Michael P. Thomas William D. Mallender Lawrence R. Dick Ping Li Hua Liao 《Cell biochemistry and biophysics》2013,67(1):139-147
Ubiquitin (Ub) and ubiquitin-like (Ubl) proteins regulate a variety of important cellular processes by forming covalent conjugates with target proteins or lipids. Ubl conjugation is catalyzed by a cascade of proteins including activating enzymes (E1), conjugating enzymes (E2), and in many cases ligation enzymes (E3). The discovery of MLN4924 (Brownell et al., Mol Cell 37: 102–111, 1), an investigational small molecule that is a mechanism-based inhibitor of NEDD8-activating enzyme (NAE), reveals a promising strategy of targeting E1/Ubl pathway for therapeutic purposes. In order to better understand, the biochemical dynamics of Ubl conjugation in cells and tissues, we have developed a mass spectrometry-based method to quantify E1 and Ubls using isotope-labeled proteins as internal standards. Furthermore, we have used the described method to quantify levels of the covalent Nedd8-inhibitor adduct formed in MLN4924 treated cells and tissues. The Nedd8–MLN4924 adduct is a tight-binding inhibitor of NAE, and its cellular concentration represents an indirect pharmacodynamic readout of NAE/Nedd8 pathway inhibition. 相似文献
996.
Yahui Shen Guixian Song Yaoqiu Liu Lijuan Zhou Hailang Liu Xiangqing Kong Yanhui Sheng Kejiang Cao Lingmei Qian 《Cell biochemistry and biophysics》2013,66(1):139-146
Fatty acid–binding protein 3 (FABP3) facilitates the movement of fatty acids in cardiac muscle. Previously, we reported that FABP3 is highly upregulated in the myocardium of ventricular septal defect patients and overexpression of FABP3 inhibited proliferation and promoted apoptosis in embryonic carcinoma cells (P19 cells). In this study, we aimed to investigate the effect of FABP3 gene silencing on P19 cell differentiation, proliferation and apoptosis. We used RNA interference and a lentiviral-based vector system to create a stable FABP3-silenced P19 cell line; knockdown of FABP3 was confirmed by quantitative real-time PCR. Expression analysis of specific differentiation marker genes using quantitative real-time PCR and observation of morphological changes using an inverted microscope revealed that knockdown of FABP3 did not significantly affect the differentiation of P19 cells into cardiomyocytes. CCK-8 proliferation assays and cell cycle analysis demonstrated that FABP3 gene silencing significantly inhibited P19 cell proliferation. Furthermore, Annexin V-FITC/propidium iodide staining and the caspase-3 activity assay revealed that FABP3 gene silencing significantly promoted serum starvation–induced apoptosis in P19 cells. In agreement with our previous research, these results demonstrate that FABP3 may play an important role during embryonic heart development, and that either overexpression or silencing of FABP3 will lead to an imbalance between proliferation and apoptosis, which may result in embryonic cardiac malformations. 相似文献
997.
998.
999.