全文获取类型
收费全文 | 9240篇 |
免费 | 700篇 |
国内免费 | 992篇 |
专业分类
10932篇 |
出版年
2024年 | 24篇 |
2023年 | 124篇 |
2022年 | 313篇 |
2021年 | 508篇 |
2020年 | 379篇 |
2019年 | 450篇 |
2018年 | 375篇 |
2017年 | 275篇 |
2016年 | 408篇 |
2015年 | 582篇 |
2014年 | 699篇 |
2013年 | 782篇 |
2012年 | 887篇 |
2011年 | 766篇 |
2010年 | 492篇 |
2009年 | 464篇 |
2008年 | 530篇 |
2007年 | 466篇 |
2006年 | 385篇 |
2005年 | 297篇 |
2004年 | 293篇 |
2003年 | 260篇 |
2002年 | 212篇 |
2001年 | 146篇 |
2000年 | 129篇 |
1999年 | 129篇 |
1998年 | 82篇 |
1997年 | 62篇 |
1996年 | 52篇 |
1995年 | 61篇 |
1994年 | 64篇 |
1993年 | 41篇 |
1992年 | 36篇 |
1991年 | 43篇 |
1990年 | 31篇 |
1989年 | 23篇 |
1988年 | 11篇 |
1987年 | 10篇 |
1986年 | 12篇 |
1985年 | 12篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
91.
Mu Dan Pan Chenyang Qi Zehua Qin Huaguang Li Qin Liang Kexin Rao Yuchun Sun Tingzhe 《Plant Growth Regulation》2021,95(1):111-120
Plant Growth Regulation - The tea green leafhopper (Empoasca onukii Matsuda) is a severe pest for the tea plant (Camellia sinensis (L.) O. Ktze), which significantly reduces the tea yields and... 相似文献
92.
Molecular Biology Reports - Chemosensory receptors in the dendritic membrane of olfactory cells are critical for the molecular recognition and discrimination of odorants. Tropidothorax elegans is a... 相似文献
93.
Neurochemical Research - Neuroblastomas (NB) are childhood malignant tumors originating in the sympathetic nervous system. MicroRNAs (miRNAs) play an essential regulatory role in tumorigenesis and... 相似文献
94.
Fang Yong E Cailing Wu Shixing Meng Zudong Qin Guifang Wang Rongying 《Cytotechnology》2021,73(6):775-785
Cytotechnology - Psoriasis is a skin disorder that is classed as an autoimmune disease. It is characterized by excessive proliferation, abnormal migration and differentiation of keratinocytes, as... 相似文献
95.
Li Jiang-Fan He Lei Deng Yong-Qiang Qi Shu-Hui Chen Yue-Hong Zhang Xiao-Lu Hu Shi-Xiong Fan Rui-Wen Zhao Guang-Yu Qin Cheng-Feng 《中国病毒学》2021,36(6):1484-1491
Virologica Sinica - The sudden emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) has caused global panic in 2003, and the risk of SARS-CoV outbreak still exists. However, no... 相似文献
96.
Jie Wang Long‐Jiang Yu Wenda Wang Qiujing Yan Tingyun Kuang Xiaochun Qin Jian‐Ren Shen 《植物学报(英文版)》2021,63(7):1367-1381
Photosystem I (PSI) is one of the two photosystems in photosynthesis, and performs a series of electron transfer reactions leading to the reduction of ferredoxin. In higher plants, PSI is surrounded by four light-harvesting complex I (LHCI) subunits, which harvest and transfer energy efficiently to the PSI core. The crystal structure of PSI-LHCI supercomplex has been analyzed up to 2.6 Å resolution, providing much information on the arrangement of proteins and cofactors in this complicated supercomplex. Here we have optimized crystallization conditions, and analyzed the crystal structure of PSI-LHCI at 2.4 Å resolution. Our structure showed some shift of the LHCI, especially the Lhca4 subunit, away from the PSI core, suggesting the indirect connection and inefficiency of energy transfer from this Lhca subunit to the PSI core. We identified five new lipids in the structure, most of them are located in the gap region between the Lhca subunits and the PSI core. These lipid molecules may play important roles in binding of the Lhca subunits to the core, as well as in the assembly of the supercomplex. The present results thus provide novel information for the elucidation of the mechanisms for the light-energy harvesting, transfer and assembly of this supercomplex. 相似文献
97.
家蝇Phormicin作为防御素家族的成员,是一类具有广普抗菌活性的抗菌肽.本研究采用原核表达法表达并纯化获得了家蝇PhormicinA蛋白.将家蝇Phormicin A原核表达蛋白与完全佐剂和不完全佐剂乳化混匀,先后免疫新西兰白兔获得其多克隆抗体.通过原核表达蛋白中和吸附实验,以及Western blot实验验证了抗体的特异性.进一步用金黄色葡萄球菌刺激家蝇三龄幼虫样品,进行内源性验证并测定抗体的效价.结果 显示,该多克隆抗体既可以识别家蝇Phormicin A原核表达蛋白,也可以识别金黄色葡萄球菌刺激家蝇三龄幼虫产生的内源性Phormicin A蛋白.本研究为进一步探索Phormicin在家蝇天然免疫和防御中的机制等后续工作打下基础. 相似文献
98.
Yong-Xin Liu Yuan Qin Tong Chen Meiping Lu Xubo Qian Xiaoxuan Guo Yang Bai 《蛋白质与细胞》2021,12(5):315-330
Advances in high-throughput sequencing(HTS)have fostered rapid developments in the field of microbiome research,and massive microbiome datasets are now being generated.However,the diversity of software tools and the complexity of analysis pipelines make it difficult to access this field.Here,we systematically summarize the advantages and limitations of micro-biome methods.Then,we recommend specific pipelines for amplicon and metagenomic analyses,and describe commonly-used software and databases,to help researchers select the appropriate tools.Furthermore,we introduce statistical and visualization methods suit-able for microbiome analysis,including alpha-and beta-diversity,taxonomic composition,difference compar-isons,correlation,networks,machine learning,evolu-tion,source tracing,and common visualization styles to help researchers make informed choices.Finally,a step-by-step reproducible analysis guide is introduced.We hope this review will allow researchers to carry out data analysis more effectively and to quickly select the appropriate tools in order to efficiently mine the bio-logical significance behind the data. 相似文献
99.
Qin Long Miki Naoko H. Zhang Guosheng Fujii Yoshiharu 《Landscape and Ecological Engineering》2021,17(3):245-251
Landscape and Ecological Engineering - Juniperus sabina L. is a native evergreen conifer of the Mu Us Sandy Land. It has prostrating stems that can prevent sand shifting and is an important... 相似文献
100.
Feifei Chen Jianren Ye Wanhui Liu Chonlong Chio Wendy Wang Wensheng Qin 《Molecular Plant Pathology》2021,22(7):843-857
Genetic transformation is a valuable and essential method that provides powerful insights into the gene function of microorganisms and contributes to the construction of engineered bacteria. Here, we developed a novel genetic transformation system to easily knock out a highly GC-rich gene (74.71% GC) from Burkholderia pyrrocinia JK-SH007, a biocontrol strain of poplar canker disease. This system revealed a reliable selectable marker (trimethoprim resistance gene, Tmp) and a simplified, efficient transformation method (6,363.64 CFU/μg, pHKT2) that was developed via freeze-thawing. The knockout recombineering of B. pyrrocinia JK-SH007 was achieved through a suicide plasmid with a three-fragment mutagenesis construct. The three-fragment cassette for mutagenesis was generated by overlap extension and touchdown PCRs and composed of Tmp flanked by GC-rich upstream and downstream fragments from B. pyrrocinia JK-SH007. The mutant strain (ΔBpEG), which was verified by PCR, lost 93.3% of its ability to degrade carboxymethyl cellulose over 40 days. Overall, this system may contribute to future research on B. pyrrocinia traits. 相似文献