新型生物——人工复合血管研制的初步报告

本文设计一种由胶原和高分子聚合物组成的新型生物一人工复合血管。其研制过程是将包绕有聚酯网的硅胶棒埋入羊的皮下组织,再将形成的经聚酯网为支架的胶原管经醛化处理。作者通过肉眼和SEM观察提出了研制生物——人工复合血管的要点:聚酯网网孔要合适,其与硅胶棒的间隙要恰当,理化处理方法更要选择好。     

Analysis of the replication region of a mycobacterial plasmid, pMSC262.

We determined the nucleotide sequence of a DNA fragment which contains the replication region of pMSC262, a Mycobacterium scrofulaceum plasmid used to construct the Mycobacterium-Escherichia coli shuttle vector. The complete sequence of the fragment contained 2,504 bp with an overall G+C content of 69.8%. By deletion analysis, we found that the minimum length required for plasmid replication in M. bovis BCG was about 1.6 kb. Within this region, several open reading frames (ORFs) and a putative replication origin (ori) were identified by computer analysis. One of the ORFs, ORF2, which encodes a putative 28.9-kDa basic protein with characteristics of DNA-binding proteins, appeared to be involved in replication of the plasmid in BCG. By separation of ORF2 and the putative ori region, it was revealed that the relative locations of ORF2 and the putative ori region are likely important for replication in BCG. No DNA or amino acid homologies were found between this replication region and that of pAL5000, another mycobacterial plasmid used for vector plasmid construction. In addition, we found that this replicon did not lead to replication in E. coli and was compatible in BCG with pAL5000-derived vector plasmid pYUB75 (R. G. Barletta, D. D. Kim, S. B. Snapper, B. R. Bloom, and W. R. Jacobs, J., J. Gen. Microbiol. 138:23-30, 1992).     

刺果番荔枝中的番荔枝内酯

从刺果番荔枝（Ａｎｎｏｎａ ｍ ｕｒｉｃａｔａ Ｌ．）的种子中分离到３ 个单四氢呋喃型番荔枝内酯类化合物,用波谱方法鉴定为海南哥纳香甲素（ｈｏｗ ｉｉｃｉｎ Ａ, Ｓ１３）、乙素（ｈｏｗ ｉｉｃｉｎ Ｂ, Ｓ５）和新化合物４－去氧海南哥纳香乙素（４－ｄｅｓｏｘｙｈｏｗ ｉｉｃｉｎ Ｂ, Ｓ２）。     

细叶黄芪叶肉原生质体发育早期几种细胞器的变化

在细叶黄芪叶肉原生质体发育早期,细胞器的变化较大。离体培养4h后,线粒体的嵴和基质物质开始增加。培养3—5天后,线粒体的数量增加5倍以上,此时可见大部分线粒体围绕细胞核分布。在培养24h后,高尔基体开始发育,它们主要分布在细胞质周边区域。多糖细胞化学染色表明,高尔基体内沉积着大量嗜银物质。培养1天后,粗面内质网开始发育。培养3天时,部分叶绿体边缘出现一些空隙结构。随着叶绿体内膜结构的消失,淀粉粒增大,叶绿体逐渐转变为造粉质体。     

津白_3侏儒小鼠垂体前叶生长激素细胞的免疫细胞化学研究

津白_３侏儒小鼠（ｄＷ~ｔ）是１９８２年从津白_３纯系小鼠（ＴＡ_３）中发现,进而培育成侏的变种。本实验应用免疫细胞化学技术,对ｄｗ￣ｔ小鼠垂体前叶生长激素（ＧＨ）细胞进行观察,并根据体视学原理进行定量分析,以探讨ｄｗ~ｔ小鼠是否存在垂体发育缺陷。实验结果显示,ｄｗ~ｔ小鼠ＧＨ细胞的体积密度（Ｖｖ）和数密度（Ｎｖ）值均低于正常对照组,Ｐ＜０．０１～０．００１。表明ｄｗ~ｔ小鼠由于垂体前叶ＧＨ细胞数量减少,导致ＧＨ分泌不足,从而形成侏儒。     

CBP-18, a Ca2+-Binding Protein in Rat Brain: Tissue Distribution and Localization

Abstract: The distribution of a novel calcium-binding protein with a molecular mass of 18 kDa (CBP-18) in the rat brain was studied by means of biochemical methods and immunohistochemistry on cryostat-sectioned tissue and compared with staining patterns of parvalbumin on adjacent sections. The biochemical analysis revealed high levels of CPB-18 in cortex and cerebellum, low levels in the lungs, and undetectable levels in all other tissues tested. Immunohistochemically, the polyclonal rabbit-derived antibody for CPB-18 showed selective affinity with periglomerular cells and dendrites in the olfactory bulb. Distinct immunostaining of scattered cells and their proximal dendrites was found in the anterior olfactory nuclei and in the perirhinal and entorhinal cortex. Strong staining of neuropil with recognizable but diffusely outlined cells was observed in the retrosplenial cortex, central amygdala, hippocampal rudiment, septum, area preoptica, hypothalamus, colliculus superior, and parabrachial nuclei. The cerebellum showed strong neuropil staining of both the molecular and the granule cell layer. Less intense neuropil staining and a few scattered cells were found in the neocortex, the remaining basal forebrain, and in the entire brainstem. Immunoreactivity was barely detectable or missing in the striatum, the hippocampus, the thalamus, and in the colliculus inferior. Thus, CPB-18 shows a unique staining pattern in the CNS, different from all other Ca²⁺-binding proteins studied so far.     

Is drift ‘directional’? Unequal breeding sex ratio revisited

Unequal breeding sex ratio can significantly reduce effective population size, allowing a rare neutral allele to jump to a high frequency through genetic drift. However, this one-way alteration to allele frequency appears inconsistent with the concept that drift is non-directional. Based on binomial sampling distribution, this study developed a method to directly and exhaustively measure drift by calculating the mean deviation of change in allele frequency, then applied it to cases of unequal breeding sex ratio. The result shows that, under those cases, (1) the mean deviation can always be divided into two halves that are equal in size but opposite in direction; (2) each half consists of one or several categories represented by various allele proportions in the rare sex; (3) this proportion is another factor that determines the outcome of drift, in addition to effective population size and allele frequency; (4) drift is non-directional on a global scale, but whether an allele will drift up or down can be predicted based on the above factors. This method enables us to dissect every component of the expected change in allele frequency caused by drift and to find out the combined effect of population size, allele frequency and allele proportion in the rarer sex under neutrality but unequal breeding sex ratio.     

鸭蛋黄颜色的生态遗传分析

1 引言 蛋黄颜色作为衡量禽蛋质量的主要经济性状之一,愈来愈受到消费者的关注,家鸡中这种性状的遗传力很低。Farnsworth等(1955)发现其遗传力为0.15,Torges报道其仅为0.05,Vanchev等(1980)估测母系遗传力为0.18,父系为0.47。因此,决定蛋黄色度主要是饲料因素,饲料中的氧化类胡萝卜素(叶黄素和玉米黄质)为蛋黄提供色素来源,能大量提供有效类胡萝卜素的饲料有藻类、紫花苜蓿、黄(白)玉米、大豆等植物,甲壳类动物、微生物(醇母)及类胡萝卜素制剂Mackey分别用墨角藻(Fucus vesiculosus)、齿缘墨角藻     

Sesquiterpenoids from the Resina Commiphora Promoting the Apoptotic Activity of PC-3 Prostate Cancer Cells

Four new germacrane-type sesquiterpenes commiphoranes M1-M4 ( 1 - 4 ) together with eighteen sesquiterpenes were isolated from the Resina Commiphora. The structures and relative configurations of new substances were determined by using spectroscopic methods. Biological activity investigation revealed that nine compounds including 7 , 9 , 14 , 16 , (+)- 17 , (−)- 17 , 18 , 19 , and 20 could induce the apoptosis of prostate cancer originated PC-3 cells, through classic apoptosis signaling pathway, even using flow cytometry showed that the compound (+)- 17 caused apoptosis of PC-3 cells more than 40 %, suggesting their potential therapeutic application in the development of novel drugs against prostate cancer.