LncRNA MEG3 inhibits the progression of prostate cancer by modulating miR‐9‐5p/QKI‐5 axis

This study was designed to detecting the influences of lncRNA MEG3 in prostate cancer. Aberrant lncRNAs expression profiles of prostate cancer were screened by microarray analysis. The qRT‐PCR and Western blot were employed to investigating the expression levels of lncRNA MEG3, miR‐9‐5p and QKI‐5. The luciferase reporter assay was utilized to testifying the interactions relationship among these molecules. Applying CCK‐8 assay, wound healing assay, transwell assay and flow cytometry in turn, the cell proliferation, migration and invasion abilities as well as apoptosis were measured respectively. LncRNA MEG3 was a down‐regulated lncRNA in prostate cancer tissues and cells and could inhibit the expression of miR‐9‐5p, whereas miR‐9‐5p down‐regulated QKI‐5 expression. Overexpressed MEG3 and QKI‐5 could decrease the abilities of proliferation, migration and invasion in prostate cancer cells effectively and increased the apoptosis rate. On the contrary, miR‐9‐5p mimics presented an opposite tendency in prostate cancer cells. Furthermore, MEG3 inhibited tumour growth and up‐regulated expression of QKI‐5 in vivo. LncRNA MEG3 was a down‐regulated lncRNA in prostate cancer and impacted the abilities of cell proliferation, migration and invasion, and cell apoptosis rate, this regulation relied on regulating miR‐9‐5p and its targeting gene QKI‐5.     

北柴胡根的发育解剖学研究

应用常规石蜡切片方法对北柴胡（Bupleurum chinense DC．）1年生主根的中部和2年生主根的根尖和中部进行了横向与纵向解剖观察,研究根的发育过程,并对其1年生与2年生主根中部的结构进行了比较分析。结果表明,北柴胡根的发育包括原分乍组织、初生分生组织、初生结构和次生生长4个发育阶段。原分生组织由3群原始细胞组成,其细胞具有典型分生组织的细胞学特征;初生分生组织包括根冠原、表皮原、皮层原和中柱原。初生结构由表皮、皮层和中柱组成。初生木质部为二原型。次生生长主要是依靠维管形成层和木栓形成层的活动来完成。其木栓形成层由中柱鞘细胞恢复分裂能力而形成。2年生北柴胡主根的基本结构与1年生的相同,但与1年生的相比,其周皮、次生韧皮部和木质部面积均增加。不同点具体表现在：2年生主根的周皮增厚;分泌道数量增加;次生木质部中木纤维成群分布,在横切面上连接成一个圆环,其维管射线明显。多为多列射线。分泌道主要分布在中柱鞘薄壁组织和次生韧皮部中,均是裂生方式发生,属次生分泌道,初生结构中无分泌道存在。     

快速纯化重组腺联病毒的受体结合捕捉方法

【目的】建立用于重组腺联病毒(AAV)纯化的受体结合捕捉方法。【方法】将AAV受体的多囊肾病(PKD)结构域1和2与类弹性蛋白多肽(ELP)在重组大肠杆菌中进行融合表达,利用相变循环(ITC)纯化ELP-PKD融合蛋白;分别用昆虫和AAV-293细胞制备rAAV-GFP,与ELP-PKD融合蛋白共孵育后进行ITC,从沉淀复合物提取病毒DNA进行PCR检测;在优化条件下利用ELP-PKD蛋白结合捕捉rAAV-GFP,利用电子显微镜观察、免疫转印和细胞感染试验进行rAAV鉴定。【结果】ELP-PKD融合蛋白获得正确、可溶性表达,ITC纯化的蛋白纯度大于90%;ELP-PKD蛋白能特异结合rAAV-GFP,结合具有p H、温度和时间依赖性,受体结合捕捉方法可在1h内完成,从两种细胞纯化rAAV-GFP的回收率分别为58%和56%;rAAV-GFP洗脱具有p H和温度依赖性,洗脱rAAV-GFP的回收率分别为46%和44%;纯化rAAV-GFP具有AAV的典型形态和结构蛋白。【结论】建立的ELP-PKD结合捕捉法可用于不同细胞源rAAV的快速纯化。     

抗菌肽对细菌杀伤作用的分子机制

抗菌肽是一类新型的抗菌物质,从最低等的生物病毒、细菌到高等的动植物都有广泛分布. 以往的研究主要集中于抗菌肽对细菌细胞膜的作用机制,已经构建了三种作用模式. 但近几年的研究表明,很多抗菌肽都能有效地穿过细菌的细胞膜,直接与胞内分子相互作用,并不引起膜的破裂. 抗菌肽根据其结构特点有着多种杀菌穿膜的机制,其后分别与胞内的靶分子如核酸,蛋白质,信号转导通路等互相作用,最终实现对细菌的杀伤作用.     

糖在猕猴精子低温冷冻保存过程中的作用

TTE或TEST防冻液在冻存猕猴精子时产生不同结果,其主要不同在于防冻液中糖成分的不同。本实验利用透射电镜技术检测这2种防冻液冻存的猕猴精子冷冻前后超微结构的变化,以说明糖在低温冻存过程中的作用。结果表明,冷冻复苏过程对精子结构产生了影响。TTE法低浊保存的猕猴精子的头部的质膜出现少许皱褶或泡化现象,但精子的顶体、核或是精子尾部的结构与鲜精的结构基本相似。猕猴精子经TEST法低温保存后,大部分精子结构则发生了明显的变化。精子膜、顶体和精子核明显泡化、损伤或破裂,精子尾部不能分辨出正常的超微结构。这提示,可能由于TTE防冻液中复杂的糖成分在降温／复苏过程对精子起到了较好的协同冷冻保护作用;而TEST防冻液中单一的糖成分不能完全保护精子避免低温损伤,低温保存过程破坏了精子的结构,并影响了复苏后精子体外存活能力与受精能力。     

产漆酶疣孢漆斑菌NF-05的分离及对偶氮染料的脱色

以木质素磺酸钠为唯一碳源的培养基对带岭凉水自然保护区土壤样品进行富集培养,涂布于愈创木酚-PDA平板。经2,2′-连氮-双(3-乙基苯并噻唑-6-磺酸)（ABTS）和丁香醛联氮（SGZ）平板检测初筛,ABTS法测定摇瓶发酵液酶活力复筛,筛选到一株漆酶高产真菌NF-05。形态学观察结合rDNA-ITS序列分析,鉴定该菌为半知菌疣孢漆斑菌Myrothecium verrucaria。该菌株在液体产酶培养基中生物量积累与产酶基本同步,发酵第5天达到产酶高峰,最高酶活力为8,375.87U/L。纯化漆酶对偶氮染料脱色研究结果表明,该酶在96h对甲基橙脱色率达到90%以上,以2,2,6,6-四甲基哌啶氧化物（TE）为介体时,48h脱色率即达90%以上;该酶在24h对橙黄Ⅰ的脱色率即达90%以上;以TE为介体时,该酶在24h即使橙黄G6完全脱色。     

土壤水分时间稳定性研究进展

土壤水分是陆地生态系统中不可或缺的组成部分,在地表水文过程中起着关键作用,连接着一系列的水文、生态、气候和地质学过程,是陆地生态系统健康运行的关键。以土壤水分时间稳定性概念为主线,从时间稳定性概念、研究方法、应用和影响因素等方面,系统阐述了土壤水分时间稳定性近年来的研究进展,探讨了代表性测点的选取标准以及土壤水分时间稳定性的影响因素。结合目前的研究进展,提出了未来的研究重点:加强多因素综合作用对土壤水分时间稳定性的研究;结合"3S"技术、计算机模拟和野外实测来研究时间稳定性的尺度问题;如何高效选择代表性测点;探讨时间稳定性概念在植被恢复区和气候敏感区的研究与应用。     

Association of the SNP rs1800925(C/T) in the Interleukin-13 Gene Promoter with Pulmonary Function in Chinese Han Patients with Idiopathic Pulmonary Fibrosis

The present report studied potential association of the rs1800925(C/T) single nucleotide polymorphism (SNP) of the Interleukin (IL)-13 gene promoter with idiopathic pulmonary fibrosis (IPF) in patients of Chinese Han ethnicity. Seventy patients with IPF were enrolled and divided into three subgroups: group A (61–79 % pred. D_LCO; n = 22), group B (51–60 % pred. D_LCO; n = 20), and group C (≤50 % pred. D_LCO; n = 28). Control group consisted of 80 healthy individuals of Chinese Han ethnicity. The SNP rs1800925(C/T) was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) analysis. The IL-13 CC genotype was present in 28/70 (40.0 %), homozygous TT in 6/70 (8.6 %) and heterozygous CT in 36/70 (51.4 %) patients with IPF. In control group, these genotypes were present in 30/80 (37.5 %), 11/80 (13.75 %), 39/80 (48.75 %), respectively, indicating that the distribution of the above three genotypes was not significantly different between patients with IPF and healthy controls. When the patients were stratified according to their D_LCO and D_LCO/VA, the frequencies of genotypes CT and TT in the groups A, B, and C were, respectively, 40.9 % (9/22), 50 % (10/20), and 82.1 % (23/28). Thus, significant differences in the distribution of alleles at ?1112 region of IL-13 gene were observed among the study groups A, B, and C, with the highest frequency in group C (p < 0.05). In conclusion, the rs1800925 T allele of the IL-13 gene is associated with worse pulmonary function in patients with IPF of Chinese Han ethnicity.